Abstract
The H-phosphonate of a derivative of thioctic acid (TA) was synthesized and used to introduce a disulfide moiety at the 5'-end of oligonucleotides. This method overcomes the difficulties experienced with the phosphoramidite approach when employing a cyclic disulfide in the starting alcohol. The disulfide-modified oligonucleotides are subsequently used in metallic nanoparticle (Au and Ag) and surface functionalisation for sensitive, sequence specific analytical detection strategies.
Original language | English |
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Pages (from-to) | 5787-5790 |
Number of pages | 3 |
Journal | Tetrahedron Letters |
Volume | 51 |
Issue number | 44 |
DOIs | |
Publication status | Published - 3 Nov 2010 |
Keywords
- H-phosphonate
- thioctic acid
- oligonucleotides
- phosphoramidite approach
- alcohol