Thermoresponsive polymer micropatterns fabricated by dip-pen nanolithography for a highly controllable substrate with potential cellular applications

Stacey Laing, Raffaella Suriano, Dimitrios A. Lamprou, Carol-Anne Smith, Matthew J. Dalby, Samuel Mabbott, Karen Faulds, Duncan Graham

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We report a novel approach for patterning thermoresponsive hydrogels based on N,N-diethylacrylamide (DEAAm) and bifunctional Jeffamine ED-600 by dip-pen nanolithography (DPN). The direct writing of micron-sized thermoresponsive polymer spots was achieved with efficient control over feature size. A Jeffamine-based ink prepared through the combination of organic polymers, such as DEAAm, in an inorganic silica network was used to print thermosensitive arrays on a thiol-silanised silicon oxide substrate. The use of a Jeffamine hydrogel, acting as a carrier matrix, allowed a reduction in the evaporation of ink molecules with high volatility, such as DEAAm, and facilitated the transfer of ink from tip to substrate. The thermoresponsive behaviour of polymer arrays which swell/de-swell in aqueous solution in response to a change in temperature was successfully characterised by atomic force microscopy (AFM) and Raman spectroscopy: a thermally-induced change in height and hydration state was observed, respectively. Finally, we demonstrate that cells can adhere to and interact with these dynamic features and exhibit a change in behaviour when cultured on the substrates above and below the transition temperature of the Jeffamine/DEAAm thermoresponsive hydrogels. This demonstrates the potential of these micropatterned hydrogels to act as a controllable surface for cell growth.
LanguageEnglish
Pages24844–24852
Number of pages9
JournalACS Applied Materials and Interfaces
Volume8
Issue number37
Early online date30 Aug 2016
DOIs
Publication statusE-pub ahead of print - 30 Aug 2016

Fingerprint

Nanolithography
Ink
Hydrogels
Polymers
Substrates
Volatilization
Organic polymers
Raman Spectrum Analysis
Transition Temperature
Atomic Force Microscopy
Hydrogel
Silicon oxides
Cell growth
Silicon
Sulfhydryl Compounds
Silicon Dioxide
Hydration
Oxides
Raman spectroscopy
Atomic force microscopy

Keywords

  • thermoresponsive polymers
  • smart hydrogel structures
  • polymer arrays
  • dip-pen nanolithography
  • atomic force microscopy

Cite this

Laing, Stacey ; Suriano, Raffaella ; Lamprou, Dimitrios A. ; Smith, Carol-Anne ; Dalby, Matthew J. ; Mabbott, Samuel ; Faulds, Karen ; Graham, Duncan. / Thermoresponsive polymer micropatterns fabricated by dip-pen nanolithography for a highly controllable substrate with potential cellular applications. In: ACS Applied Materials and Interfaces. 2016 ; Vol. 8, No. 37. pp. 24844–24852.
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title = "Thermoresponsive polymer micropatterns fabricated by dip-pen nanolithography for a highly controllable substrate with potential cellular applications",
abstract = "We report a novel approach for patterning thermoresponsive hydrogels based on N,N-diethylacrylamide (DEAAm) and bifunctional Jeffamine ED-600 by dip-pen nanolithography (DPN). The direct writing of micron-sized thermoresponsive polymer spots was achieved with efficient control over feature size. A Jeffamine-based ink prepared through the combination of organic polymers, such as DEAAm, in an inorganic silica network was used to print thermosensitive arrays on a thiol-silanised silicon oxide substrate. The use of a Jeffamine hydrogel, acting as a carrier matrix, allowed a reduction in the evaporation of ink molecules with high volatility, such as DEAAm, and facilitated the transfer of ink from tip to substrate. The thermoresponsive behaviour of polymer arrays which swell/de-swell in aqueous solution in response to a change in temperature was successfully characterised by atomic force microscopy (AFM) and Raman spectroscopy: a thermally-induced change in height and hydration state was observed, respectively. Finally, we demonstrate that cells can adhere to and interact with these dynamic features and exhibit a change in behaviour when cultured on the substrates above and below the transition temperature of the Jeffamine/DEAAm thermoresponsive hydrogels. This demonstrates the potential of these micropatterned hydrogels to act as a controllable surface for cell growth.",
keywords = "thermoresponsive polymers, smart hydrogel structures, polymer arrays, dip-pen nanolithography, atomic force microscopy",
author = "Stacey Laing and Raffaella Suriano and Lamprou, {Dimitrios A.} and Carol-Anne Smith and Dalby, {Matthew J.} and Samuel Mabbott and Karen Faulds and Duncan Graham",
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Thermoresponsive polymer micropatterns fabricated by dip-pen nanolithography for a highly controllable substrate with potential cellular applications. / Laing, Stacey; Suriano, Raffaella; Lamprou, Dimitrios A.; Smith, Carol-Anne; Dalby, Matthew J.; Mabbott, Samuel; Faulds, Karen; Graham, Duncan.

In: ACS Applied Materials and Interfaces, Vol. 8, No. 37, 30.08.2016, p. 24844–24852.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Thermoresponsive polymer micropatterns fabricated by dip-pen nanolithography for a highly controllable substrate with potential cellular applications

AU - Laing, Stacey

AU - Suriano, Raffaella

AU - Lamprou, Dimitrios A.

AU - Smith, Carol-Anne

AU - Dalby, Matthew J.

AU - Mabbott, Samuel

AU - Faulds, Karen

AU - Graham, Duncan

N1 - Laing, S., Suriano, R., Lamprou, D. A., Smith, C-A., Dalby, M. J., Mabbott, S., ... Graham, D. (2016). Thermoresponsive polymer micropatterns fabricated by dip-pen nanolithography for a highly controllable substrate with potential cellular applications. ACS Applied Materials and Interfaces, 1-33. Copyright © 2016 American Chemical Society

PY - 2016/8/30

Y1 - 2016/8/30

N2 - We report a novel approach for patterning thermoresponsive hydrogels based on N,N-diethylacrylamide (DEAAm) and bifunctional Jeffamine ED-600 by dip-pen nanolithography (DPN). The direct writing of micron-sized thermoresponsive polymer spots was achieved with efficient control over feature size. A Jeffamine-based ink prepared through the combination of organic polymers, such as DEAAm, in an inorganic silica network was used to print thermosensitive arrays on a thiol-silanised silicon oxide substrate. The use of a Jeffamine hydrogel, acting as a carrier matrix, allowed a reduction in the evaporation of ink molecules with high volatility, such as DEAAm, and facilitated the transfer of ink from tip to substrate. The thermoresponsive behaviour of polymer arrays which swell/de-swell in aqueous solution in response to a change in temperature was successfully characterised by atomic force microscopy (AFM) and Raman spectroscopy: a thermally-induced change in height and hydration state was observed, respectively. Finally, we demonstrate that cells can adhere to and interact with these dynamic features and exhibit a change in behaviour when cultured on the substrates above and below the transition temperature of the Jeffamine/DEAAm thermoresponsive hydrogels. This demonstrates the potential of these micropatterned hydrogels to act as a controllable surface for cell growth.

AB - We report a novel approach for patterning thermoresponsive hydrogels based on N,N-diethylacrylamide (DEAAm) and bifunctional Jeffamine ED-600 by dip-pen nanolithography (DPN). The direct writing of micron-sized thermoresponsive polymer spots was achieved with efficient control over feature size. A Jeffamine-based ink prepared through the combination of organic polymers, such as DEAAm, in an inorganic silica network was used to print thermosensitive arrays on a thiol-silanised silicon oxide substrate. The use of a Jeffamine hydrogel, acting as a carrier matrix, allowed a reduction in the evaporation of ink molecules with high volatility, such as DEAAm, and facilitated the transfer of ink from tip to substrate. The thermoresponsive behaviour of polymer arrays which swell/de-swell in aqueous solution in response to a change in temperature was successfully characterised by atomic force microscopy (AFM) and Raman spectroscopy: a thermally-induced change in height and hydration state was observed, respectively. Finally, we demonstrate that cells can adhere to and interact with these dynamic features and exhibit a change in behaviour when cultured on the substrates above and below the transition temperature of the Jeffamine/DEAAm thermoresponsive hydrogels. This demonstrates the potential of these micropatterned hydrogels to act as a controllable surface for cell growth.

KW - thermoresponsive polymers

KW - smart hydrogel structures

KW - polymer arrays

KW - dip-pen nanolithography

KW - atomic force microscopy

UR - http://pubs.acs.org/journal/aamick

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M3 - Article

VL - 8

SP - 24844

EP - 24852

JO - ACS Applied Materials and Interfaces

T2 - ACS Applied Materials and Interfaces

JF - ACS Applied Materials and Interfaces

SN - 1944-8244

IS - 37

ER -