Abstract
| Language | English |
|---|---|
| Pages | 750-753 |
| Number of pages | 3 |
| Journal | Clinical Biochemistry |
| Volume | 43 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 30 Jun 2010 |
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Keywords
- prolactin
- polyethylene glycols
- humans
- reproducibility of results
- magnetic resonance spectroscopy
- temperature
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Thermal degradation of polyethylene glycol 6000 and its effect on the assay of macroprolactin. / Boughen, Louise; Liggat, John; Ellis, Graham.
In: Clinical Biochemistry, Vol. 43, No. 9, 30.06.2010, p. 750-753.Research output: Contribution to journal › Article
TY - JOUR
T1 - Thermal degradation of polyethylene glycol 6000 and its effect on the assay of macroprolactin
AU - Boughen, Louise
AU - Liggat, John
AU - Ellis, Graham
PY - 2010/6/30
Y1 - 2010/6/30
N2 - Study of the effectiveness of partially degraded polyethylene glycol 6000 (PEG) as a precipitant for macroprolactin. PEG was heated to 63 °C in air for up to 20 days and its effectiveness assessed as a precipitant for sera containing normal prolactin or macroprolactin. Decomposition was studied chemically and with NMR spectroscopy. Thermal degradation was similar to what had occurred over several years of natural degradation. Initially PEG degraded 2-5 days caused excess precipitation of monomeric prolactin (false-positive macroprolactinemia). Samples degraded 18-20 days failed to precipitate macroprolactin, giving false negative results. Two 1H NMR peaks at 4-4.5 ppm were not detectable in undegraded PEG but were after 1 day. Their relative integral increased to 20 days. Aging of PEG can be accelerated by heating. The suitability of PEG for use in macroprolactin assays can be assessed by the absence of peaks at 4-4.5 ppm by 1H NMR.
AB - Study of the effectiveness of partially degraded polyethylene glycol 6000 (PEG) as a precipitant for macroprolactin. PEG was heated to 63 °C in air for up to 20 days and its effectiveness assessed as a precipitant for sera containing normal prolactin or macroprolactin. Decomposition was studied chemically and with NMR spectroscopy. Thermal degradation was similar to what had occurred over several years of natural degradation. Initially PEG degraded 2-5 days caused excess precipitation of monomeric prolactin (false-positive macroprolactinemia). Samples degraded 18-20 days failed to precipitate macroprolactin, giving false negative results. Two 1H NMR peaks at 4-4.5 ppm were not detectable in undegraded PEG but were after 1 day. Their relative integral increased to 20 days. Aging of PEG can be accelerated by heating. The suitability of PEG for use in macroprolactin assays can be assessed by the absence of peaks at 4-4.5 ppm by 1H NMR.
KW - prolactin
KW - polyethylene glycols
KW - humans
KW - reproducibility of results
KW - magnetic resonance spectroscopy
KW - temperature
UR - http://www.scopus.com/inward/record.url?scp=77952956286&partnerID=8YFLogxK
UR - http://www.sciencedirect.com/science/journal/00099120
U2 - 10.1016/j.clinbiochem.2010.02.012
DO - 10.1016/j.clinbiochem.2010.02.012
M3 - Article
VL - 43
SP - 750
EP - 753
JO - Clinical Biochemistry
T2 - Clinical Biochemistry
JF - Clinical Biochemistry
SN - 0009-9120
IS - 9
ER -