The viability and function of primary rat hepatocytes cultured on polymeric membranes developed for hybrid artificial liver devices

M.H. Grant, C. Morgan, C.J. Henderson, G. Malsch, B. Seifert, W. Albrecht, T. Groth

    Research output: Contribution to journalArticle

    18 Citations (Scopus)

    Abstract

    Bioartificial liver devices require membranes to support the function and viability of hepatocytes because they are anchorage-dependent cells. This study investigated the ability of several polymeric membranes to support the functions of primary hepatocyte cultures. Tailor-made membranes were sought by synthesizing acrylonitrile copolymers with different comonomers resulting in ionic, hydrophilic, or reactive functional groups on the polymer surface. Hepatocyte morphology and viability were assessed by confocal microscopy, and function by the content and activities of cytochrome P450, and the expression of glutathione S-transferases. Hydrophilic membranes (polyacrylonitrile and acrylonitrile copolymerized with 2-acrylamino-2-methyl-propane sulfonic acid) were more biocompatible than hydrophobic membranes such as polysulfone. The chemistry of the hydrophilic group was important; amine groups had a deleterious effect on maintenance of the primary hepatocytes. The biocompatibility of hydrophobic membranes was improved by collagen coating. Improving the chemistry of membranes for artificial liver devices will enhance the phenotypic stability of the cells, enabling us to prolong treatment times for patients.
    LanguageEnglish
    Pages367-375
    Number of pages8
    JournalJournal of Biomedical Materials Research
    Volume73A
    Issue number3
    DOIs
    Publication statusPublished - 2005

    Fingerprint

    Polymeric membranes
    Liver
    Rats
    Membranes
    Acrylonitrile
    Propane
    Polysulfones
    Sulfonic Acids
    Confocal microscopy
    Polyacrylonitriles
    Glutathione Transferase
    Biocompatibility
    Collagen
    Cytochrome P-450 Enzyme System
    Functional groups
    Amines
    Hepatocytes
    Polymers
    Copolymers
    Coatings

    Keywords

    • liver
    • membranes
    • bioengineering
    • biomechanics
    • medicine

    Cite this

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    abstract = "Bioartificial liver devices require membranes to support the function and viability of hepatocytes because they are anchorage-dependent cells. This study investigated the ability of several polymeric membranes to support the functions of primary hepatocyte cultures. Tailor-made membranes were sought by synthesizing acrylonitrile copolymers with different comonomers resulting in ionic, hydrophilic, or reactive functional groups on the polymer surface. Hepatocyte morphology and viability were assessed by confocal microscopy, and function by the content and activities of cytochrome P450, and the expression of glutathione S-transferases. Hydrophilic membranes (polyacrylonitrile and acrylonitrile copolymerized with 2-acrylamino-2-methyl-propane sulfonic acid) were more biocompatible than hydrophobic membranes such as polysulfone. The chemistry of the hydrophilic group was important; amine groups had a deleterious effect on maintenance of the primary hepatocytes. The biocompatibility of hydrophobic membranes was improved by collagen coating. Improving the chemistry of membranes for artificial liver devices will enhance the phenotypic stability of the cells, enabling us to prolong treatment times for patients.",
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    The viability and function of primary rat hepatocytes cultured on polymeric membranes developed for hybrid artificial liver devices. / Grant, M.H.; Morgan, C.; Henderson, C.J.; Malsch, G.; Seifert, B.; Albrecht, W.; Groth, T.

    In: Journal of Biomedical Materials Research, Vol. 73A, No. 3, 2005, p. 367-375.

    Research output: Contribution to journalArticle

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    AU - Morgan, C.

    AU - Henderson, C.J.

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    AU - Seifert, B.

    AU - Albrecht, W.

    AU - Groth, T.

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    AB - Bioartificial liver devices require membranes to support the function and viability of hepatocytes because they are anchorage-dependent cells. This study investigated the ability of several polymeric membranes to support the functions of primary hepatocyte cultures. Tailor-made membranes were sought by synthesizing acrylonitrile copolymers with different comonomers resulting in ionic, hydrophilic, or reactive functional groups on the polymer surface. Hepatocyte morphology and viability were assessed by confocal microscopy, and function by the content and activities of cytochrome P450, and the expression of glutathione S-transferases. Hydrophilic membranes (polyacrylonitrile and acrylonitrile copolymerized with 2-acrylamino-2-methyl-propane sulfonic acid) were more biocompatible than hydrophobic membranes such as polysulfone. The chemistry of the hydrophilic group was important; amine groups had a deleterious effect on maintenance of the primary hepatocytes. The biocompatibility of hydrophobic membranes was improved by collagen coating. Improving the chemistry of membranes for artificial liver devices will enhance the phenotypic stability of the cells, enabling us to prolong treatment times for patients.

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