Abstract
| Language | English |
|---|---|
| Pages | 367-375 |
| Number of pages | 8 |
| Journal | Journal of Biomedical Materials Research |
| Volume | 73A |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2005 |
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Keywords
- liver
- membranes
- bioengineering
- biomechanics
- medicine
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The viability and function of primary rat hepatocytes cultured on polymeric membranes developed for hybrid artificial liver devices. / Grant, M.H.; Morgan, C.; Henderson, C.J.; Malsch, G.; Seifert, B.; Albrecht, W.; Groth, T.
In: Journal of Biomedical Materials Research, Vol. 73A, No. 3, 2005, p. 367-375.Research output: Contribution to journal › Article
TY - JOUR
T1 - The viability and function of primary rat hepatocytes cultured on polymeric membranes developed for hybrid artificial liver devices
AU - Grant, M.H.
AU - Morgan, C.
AU - Henderson, C.J.
AU - Malsch, G.
AU - Seifert, B.
AU - Albrecht, W.
AU - Groth, T.
PY - 2005
Y1 - 2005
N2 - Bioartificial liver devices require membranes to support the function and viability of hepatocytes because they are anchorage-dependent cells. This study investigated the ability of several polymeric membranes to support the functions of primary hepatocyte cultures. Tailor-made membranes were sought by synthesizing acrylonitrile copolymers with different comonomers resulting in ionic, hydrophilic, or reactive functional groups on the polymer surface. Hepatocyte morphology and viability were assessed by confocal microscopy, and function by the content and activities of cytochrome P450, and the expression of glutathione S-transferases. Hydrophilic membranes (polyacrylonitrile and acrylonitrile copolymerized with 2-acrylamino-2-methyl-propane sulfonic acid) were more biocompatible than hydrophobic membranes such as polysulfone. The chemistry of the hydrophilic group was important; amine groups had a deleterious effect on maintenance of the primary hepatocytes. The biocompatibility of hydrophobic membranes was improved by collagen coating. Improving the chemistry of membranes for artificial liver devices will enhance the phenotypic stability of the cells, enabling us to prolong treatment times for patients.
AB - Bioartificial liver devices require membranes to support the function and viability of hepatocytes because they are anchorage-dependent cells. This study investigated the ability of several polymeric membranes to support the functions of primary hepatocyte cultures. Tailor-made membranes were sought by synthesizing acrylonitrile copolymers with different comonomers resulting in ionic, hydrophilic, or reactive functional groups on the polymer surface. Hepatocyte morphology and viability were assessed by confocal microscopy, and function by the content and activities of cytochrome P450, and the expression of glutathione S-transferases. Hydrophilic membranes (polyacrylonitrile and acrylonitrile copolymerized with 2-acrylamino-2-methyl-propane sulfonic acid) were more biocompatible than hydrophobic membranes such as polysulfone. The chemistry of the hydrophilic group was important; amine groups had a deleterious effect on maintenance of the primary hepatocytes. The biocompatibility of hydrophobic membranes was improved by collagen coating. Improving the chemistry of membranes for artificial liver devices will enhance the phenotypic stability of the cells, enabling us to prolong treatment times for patients.
KW - liver
KW - membranes
KW - bioengineering
KW - biomechanics
KW - medicine
UR - http://dx.doi.org/10.1002/jbm.a.30306
U2 - 10.1002/jbm.a.30306
DO - 10.1002/jbm.a.30306
M3 - Article
VL - 73A
SP - 367
EP - 375
JO - Journal of Biomedical Materials Research
T2 - Journal of Biomedical Materials Research
JF - Journal of Biomedical Materials Research
SN - 0021-9304
IS - 3
ER -