The use of the chick biventer cervicis preparation to assess the protective activity of six international reference antivenoms on the neuromuscular effects of snake venoms in vitro

A Barfaraz, A L Harvey

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The protective activity of some antivenoms on the neuromuscular activity in vitro of six snake venoms was established in order to test the feasibility of using a simple isolated nerve-muscle preparation to compare different antivenoms. Six venoms designated as International Reference Venoms by the World Health Organization (WHO) were used: Echis carinatus (Iran), Echis carinatus (Mali), Naja naja kaouthia, Notechis scutatus, Trimeresurus flavoviridis, and Vipera russelli (Thailand). The chick biventer cervicis nerve-muscle preparation was used to detect neurotoxic and myotoxic effects. The ratio of the amount of antivenom needed to neutralize a dose of venom (w/w) was calculated in order to quantify the potencies of the antivenoms. The antivenom to venom ratios were 1000 for Echis carinatus (Mali) and for Notechis scutatus; 100 for Naja naja kaouthia and Vipera russelli; and 10 for Echis carinatus (Iran) and Trimeresurus falvoviridis. It is concluded that in vitro assays using the chick biventer cervicis preparation could be used to compare the relative potencies of different antivenoms at neutralizing myotoxic and neurotoxic toxins in snake venoms.
LanguageEnglish
Pages267-272
Number of pages6
JournalToxicon
Volume32
Issue number3
DOIs
Publication statusPublished - Mar 1994

Fingerprint

Neuromuscular Agents
Antivenins
Snake Venoms
Venoms
Trimeresurus
Mali
Elapidae
Neuromuscular Junction
Iran
Muscle
Thailand
In Vitro Techniques
Assays
Health

Keywords

  • acetylcholine
  • animals
  • antivenins
  • carbachol
  • chickens
  • drug interactions
  • muscle contraction
  • muscles
  • neuromuscular junction
  • potassium chloride
  • snake venoms
  • snakes
  • species specificity

Cite this

@article{e87cd32225b2479bb4892f8aabd3d5ba,
title = "The use of the chick biventer cervicis preparation to assess the protective activity of six international reference antivenoms on the neuromuscular effects of snake venoms in vitro",
abstract = "The protective activity of some antivenoms on the neuromuscular activity in vitro of six snake venoms was established in order to test the feasibility of using a simple isolated nerve-muscle preparation to compare different antivenoms. Six venoms designated as International Reference Venoms by the World Health Organization (WHO) were used: Echis carinatus (Iran), Echis carinatus (Mali), Naja naja kaouthia, Notechis scutatus, Trimeresurus flavoviridis, and Vipera russelli (Thailand). The chick biventer cervicis nerve-muscle preparation was used to detect neurotoxic and myotoxic effects. The ratio of the amount of antivenom needed to neutralize a dose of venom (w/w) was calculated in order to quantify the potencies of the antivenoms. The antivenom to venom ratios were 1000 for Echis carinatus (Mali) and for Notechis scutatus; 100 for Naja naja kaouthia and Vipera russelli; and 10 for Echis carinatus (Iran) and Trimeresurus falvoviridis. It is concluded that in vitro assays using the chick biventer cervicis preparation could be used to compare the relative potencies of different antivenoms at neutralizing myotoxic and neurotoxic toxins in snake venoms.",
keywords = "acetylcholine, animals, antivenins, carbachol, chickens, drug interactions, muscle contraction, muscles, neuromuscular junction, potassium chloride, snake venoms, snakes, species specificity",
author = "A Barfaraz and Harvey, {A L}",
year = "1994",
month = "3",
doi = "10.1016/0041-0101(94)90079-5",
language = "English",
volume = "32",
pages = "267--272",
journal = "Toxicon",
issn = "0041-0101",
number = "3",

}

TY - JOUR

T1 - The use of the chick biventer cervicis preparation to assess the protective activity of six international reference antivenoms on the neuromuscular effects of snake venoms in vitro

AU - Barfaraz, A

AU - Harvey, A L

PY - 1994/3

Y1 - 1994/3

N2 - The protective activity of some antivenoms on the neuromuscular activity in vitro of six snake venoms was established in order to test the feasibility of using a simple isolated nerve-muscle preparation to compare different antivenoms. Six venoms designated as International Reference Venoms by the World Health Organization (WHO) were used: Echis carinatus (Iran), Echis carinatus (Mali), Naja naja kaouthia, Notechis scutatus, Trimeresurus flavoviridis, and Vipera russelli (Thailand). The chick biventer cervicis nerve-muscle preparation was used to detect neurotoxic and myotoxic effects. The ratio of the amount of antivenom needed to neutralize a dose of venom (w/w) was calculated in order to quantify the potencies of the antivenoms. The antivenom to venom ratios were 1000 for Echis carinatus (Mali) and for Notechis scutatus; 100 for Naja naja kaouthia and Vipera russelli; and 10 for Echis carinatus (Iran) and Trimeresurus falvoviridis. It is concluded that in vitro assays using the chick biventer cervicis preparation could be used to compare the relative potencies of different antivenoms at neutralizing myotoxic and neurotoxic toxins in snake venoms.

AB - The protective activity of some antivenoms on the neuromuscular activity in vitro of six snake venoms was established in order to test the feasibility of using a simple isolated nerve-muscle preparation to compare different antivenoms. Six venoms designated as International Reference Venoms by the World Health Organization (WHO) were used: Echis carinatus (Iran), Echis carinatus (Mali), Naja naja kaouthia, Notechis scutatus, Trimeresurus flavoviridis, and Vipera russelli (Thailand). The chick biventer cervicis nerve-muscle preparation was used to detect neurotoxic and myotoxic effects. The ratio of the amount of antivenom needed to neutralize a dose of venom (w/w) was calculated in order to quantify the potencies of the antivenoms. The antivenom to venom ratios were 1000 for Echis carinatus (Mali) and for Notechis scutatus; 100 for Naja naja kaouthia and Vipera russelli; and 10 for Echis carinatus (Iran) and Trimeresurus falvoviridis. It is concluded that in vitro assays using the chick biventer cervicis preparation could be used to compare the relative potencies of different antivenoms at neutralizing myotoxic and neurotoxic toxins in snake venoms.

KW - acetylcholine

KW - animals

KW - antivenins

KW - carbachol

KW - chickens

KW - drug interactions

KW - muscle contraction

KW - muscles

KW - neuromuscular junction

KW - potassium chloride

KW - snake venoms

KW - snakes

KW - species specificity

U2 - 10.1016/0041-0101(94)90079-5

DO - 10.1016/0041-0101(94)90079-5

M3 - Article

VL - 32

SP - 267

EP - 272

JO - Toxicon

T2 - Toxicon

JF - Toxicon

SN - 0041-0101

IS - 3

ER -