The Shigella ProU system is required for osmotic tolerance and virulence

Shigella ProU in osmotic tolerance and virulence

Rasha Y. Mahmoud, Wenqin Li, Ramadan A. Eldomany, Mohamed Emara, Jun Yu

Research output: Contribution to journalArticle

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Abstract

To cope with hyperosmotic stress encountered in the environments and in the host, the pathogenic as well as non-pathogenic microbes use diverse transport systems to obtain osmoprotectants. To study the role of Shigella sonnei ProU system in response to hyperosmotic stress and virulence, we constructed deletion and complementation strains of proV and used an RNAi approach to silence the whole ProU operon. We compared the response between wild type and the mutants to the hyperosmotic pressure in vitro, and assessed virulence properties of the mutants using gentamicin protection assay as well as Galleria mellonella moth larvae model. In response to osmotic stress by either NaCl or KCl, S. sonnei highly up-regulates transcription of proVWX genes. Supplementation of betaine greatly elevates the growth of the wild type S. sonnei but not the proV mutants in M9 medium containing 0.2 M NaCl or 0.2 M KCl. The proV mutants are also defective in intracellular growth compared with the wild type. The moth larvae model of G. mellonella shows that either deletion of proV gene or knockdown of proVWX transcripts by RNAi significantly attenuates virulence. ProU system in S. sonnei is required to cope with osmotic stress for survival and multiplication in vitro and ex vivo, and for infection.
Original languageEnglish
Article number2016VIRULENCE0129R
Pages (from-to)1-27
Number of pages27
JournalVirulence
Publication statusAccepted/In press - 18 Aug 2016

Fingerprint

Shigella sonnei
Shigella
Virulence
Moths
RNA Interference
Larva
Gene Knockdown Techniques
Osmoregulation
Betaine
Osmotic Pressure
Operon
Growth
Gentamicins
Up-Regulation
Pressure
Infection
Genes
In Vitro Techniques

Keywords

  • Shigella sonnei
  • ProU
  • osmoprotectants
  • osmotic tolerance
  • RNAi
  • hyperosmotic stress
  • virulence

Cite this

Mahmoud, R. Y., Li, W., Eldomany, R. A., Emara, M., & Yu, J. (Accepted/In press). The Shigella ProU system is required for osmotic tolerance and virulence: Shigella ProU in osmotic tolerance and virulence. Virulence, 1-27. [2016VIRULENCE0129R].
Mahmoud, Rasha Y. ; Li, Wenqin ; Eldomany, Ramadan A. ; Emara, Mohamed ; Yu, Jun. / The Shigella ProU system is required for osmotic tolerance and virulence : Shigella ProU in osmotic tolerance and virulence. In: Virulence. 2016 ; pp. 1-27.
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The Shigella ProU system is required for osmotic tolerance and virulence : Shigella ProU in osmotic tolerance and virulence. / Mahmoud, Rasha Y.; Li, Wenqin; Eldomany, Ramadan A.; Emara, Mohamed ; Yu, Jun.

In: Virulence, 18.08.2016, p. 1-27.

Research output: Contribution to journalArticle

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