The role of phosphatidylinositol 4,5 bisphosphate breakdown in cell-surface receptor activation

C J Kirk, E A Bone, S Palmer, R H Michell

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The activation of Ca2+-mobilising receptors on hepatocytes and many other cells leads to a prompt reduction in the cellular content of inositol phospholipids. The primary event which underlies these changes is, most probably, a phospholipase C-catalysed attack upon phosphatidylinositol 4,5 bisphosphate. The receptor-mediated breakdown of this lipid in stimulated cells is: (i) not mediated by an increase in cytosol [Ca2+] and (ii) closely coupled to receptor occupation. Phosphatidylinositol 4,5 bisphosphate degradation may be studied by measuring the appearance of the water-soluble product, inositol trisphosphate (and its metabolites: inositol bisphosphate and inositol monophosphate), in stimulated cells. Recent evidence indicates that inositol trisphosphate and the lipid soluble product of phosphatidylinositol 4,5 bisphosphate breakdown, 1,2 diacylglycerol, may act as 'second messengers' which mediate the effects of many extracellular signals in stimulated cells.

LanguageEnglish
Pages489-504
Number of pages16
JournalJournal of Receptor Research
Volume4
Issue number1-6
Publication statusPublished - 1984
Externally publishedYes

Fingerprint

Cell Surface Receptors
Inositol
Phosphatidylinositols
Lipids
Type C Phospholipases
Second Messenger Systems
Cytosol
Hepatocytes
Water

Keywords

  • animals
  • calcium
  • ganglia, sympathetic
  • liver
  • phosphatidylinositol 4,5-diphosphate
  • phosphatidylinositols
  • rats
  • receptors, cell surface
  • vasopressins

Cite this

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title = "The role of phosphatidylinositol 4,5 bisphosphate breakdown in cell-surface receptor activation",
abstract = "The activation of Ca2+-mobilising receptors on hepatocytes and many other cells leads to a prompt reduction in the cellular content of inositol phospholipids. The primary event which underlies these changes is, most probably, a phospholipase C-catalysed attack upon phosphatidylinositol 4,5 bisphosphate. The receptor-mediated breakdown of this lipid in stimulated cells is: (i) not mediated by an increase in cytosol [Ca2+] and (ii) closely coupled to receptor occupation. Phosphatidylinositol 4,5 bisphosphate degradation may be studied by measuring the appearance of the water-soluble product, inositol trisphosphate (and its metabolites: inositol bisphosphate and inositol monophosphate), in stimulated cells. Recent evidence indicates that inositol trisphosphate and the lipid soluble product of phosphatidylinositol 4,5 bisphosphate breakdown, 1,2 diacylglycerol, may act as 'second messengers' which mediate the effects of many extracellular signals in stimulated cells.",
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journal = "Journal of Receptors and Signal Transduction",
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The role of phosphatidylinositol 4,5 bisphosphate breakdown in cell-surface receptor activation. / Kirk, C J; Bone, E A; Palmer, S; Michell, R H.

In: Journal of Receptor Research, Vol. 4, No. 1-6, 1984, p. 489-504.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The role of phosphatidylinositol 4,5 bisphosphate breakdown in cell-surface receptor activation

AU - Kirk, C J

AU - Bone, E A

AU - Palmer, S

AU - Michell, R H

PY - 1984

Y1 - 1984

N2 - The activation of Ca2+-mobilising receptors on hepatocytes and many other cells leads to a prompt reduction in the cellular content of inositol phospholipids. The primary event which underlies these changes is, most probably, a phospholipase C-catalysed attack upon phosphatidylinositol 4,5 bisphosphate. The receptor-mediated breakdown of this lipid in stimulated cells is: (i) not mediated by an increase in cytosol [Ca2+] and (ii) closely coupled to receptor occupation. Phosphatidylinositol 4,5 bisphosphate degradation may be studied by measuring the appearance of the water-soluble product, inositol trisphosphate (and its metabolites: inositol bisphosphate and inositol monophosphate), in stimulated cells. Recent evidence indicates that inositol trisphosphate and the lipid soluble product of phosphatidylinositol 4,5 bisphosphate breakdown, 1,2 diacylglycerol, may act as 'second messengers' which mediate the effects of many extracellular signals in stimulated cells.

AB - The activation of Ca2+-mobilising receptors on hepatocytes and many other cells leads to a prompt reduction in the cellular content of inositol phospholipids. The primary event which underlies these changes is, most probably, a phospholipase C-catalysed attack upon phosphatidylinositol 4,5 bisphosphate. The receptor-mediated breakdown of this lipid in stimulated cells is: (i) not mediated by an increase in cytosol [Ca2+] and (ii) closely coupled to receptor occupation. Phosphatidylinositol 4,5 bisphosphate degradation may be studied by measuring the appearance of the water-soluble product, inositol trisphosphate (and its metabolites: inositol bisphosphate and inositol monophosphate), in stimulated cells. Recent evidence indicates that inositol trisphosphate and the lipid soluble product of phosphatidylinositol 4,5 bisphosphate breakdown, 1,2 diacylglycerol, may act as 'second messengers' which mediate the effects of many extracellular signals in stimulated cells.

KW - animals

KW - calcium

KW - ganglia, sympathetic

KW - liver

KW - phosphatidylinositol 4,5-diphosphate

KW - phosphatidylinositols

KW - rats

KW - receptors, cell surface

KW - vasopressins

M3 - Article

VL - 4

SP - 489

EP - 504

JO - Journal of Receptors and Signal Transduction

T2 - Journal of Receptors and Signal Transduction

JF - Journal of Receptors and Signal Transduction

SN - 1079-9893

IS - 1-6

ER -