The role of phosphatidylinositol 4,5 bisphosphate breakdown in cell-surface receptor activation

C J Kirk, E A Bone, S Palmer, R H Michell

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The activation of Ca2+-mobilising receptors on hepatocytes and many other cells leads to a prompt reduction in the cellular content of inositol phospholipids. The primary event which underlies these changes is, most probably, a phospholipase C-catalysed attack upon phosphatidylinositol 4,5 bisphosphate. The receptor-mediated breakdown of this lipid in stimulated cells is: (i) not mediated by an increase in cytosol [Ca2+] and (ii) closely coupled to receptor occupation. Phosphatidylinositol 4,5 bisphosphate degradation may be studied by measuring the appearance of the water-soluble product, inositol trisphosphate (and its metabolites: inositol bisphosphate and inositol monophosphate), in stimulated cells. Recent evidence indicates that inositol trisphosphate and the lipid soluble product of phosphatidylinositol 4,5 bisphosphate breakdown, 1,2 diacylglycerol, may act as 'second messengers' which mediate the effects of many extracellular signals in stimulated cells.

Original languageEnglish
Pages (from-to)489-504
Number of pages16
JournalJournal of Receptor Research
Volume4
Issue number1-6
Publication statusPublished - 1984
Externally publishedYes

Keywords

  • animals
  • calcium
  • ganglia, sympathetic
  • liver
  • phosphatidylinositol 4,5-diphosphate
  • phosphatidylinositols
  • rats
  • receptors, cell surface
  • vasopressins

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