The resolution of oligosaccharides by high ph anion-exchange chromatography

J M McGuire, M A Elliott, H G Elliott, K D Smith

Research output: Contribution to journalComment/debate

5 Citations (Scopus)

Abstract

The natural heterogeneity of the oligosaccharide chains of glycoproteins requires that chromatography be an essential part of structural elucidation. Until recently, the rapid analysis of underivatised carbohydrates by HPLC was hampered, due to the extensive structural heterogeneity, by the absence of a chromatographic medium for satisfactory separation and by the lack of a sufficiently sensitive detection system. This problem has been overcome by the introduction of high pH anion exchange chromatography (HPAEC) with pulsed electrochemical detection (PED) which separates oligosaccharides as their oxyanions on micropellicular quaternary ammonium resins [1-10]. This technique resolves the linkage and branch isomers of neutral and anionic oligosaccharides to picomole sensitivity without pre- or post-column derivatisation. The DX-500 chromatography system is viewed as a major innovation in HPAEC-PED due to an improved sensitivity and resolution as a result of a new amperometric cell design and new digital detector signal processing. Our investigations have utilised the DX-500 in the analysis of various oligosaccharide mixtures in direct comparison with the DX-300 system that it has superceded.
LanguageEnglish
Pages63-69
Number of pages7
JournalCarbohydrate Research
Volume270
Issue number1
DOIs
Publication statusPublished - 18 Apr 1995

Fingerprint

Chromatography
Oligosaccharides
Anions
Computer-Assisted Signal Processing
Ammonium Compounds
Isomers
Glycoproteins
Signal processing
Resins
Innovation
High Pressure Liquid Chromatography
Carbohydrates
Detectors

Keywords

  • oligosaccharides
  • high ph anion exchange chromatography (HPAEC)
  • alpha(1) acid glycoprotein
  • fetuin
  • immunoglobulin G
  • pulsed-amperometric detection
  • monosaccharide analysis
  • separation

Cite this

McGuire, J M ; Elliott, M A ; Elliott, H G ; Smith, K D . / The resolution of oligosaccharides by high ph anion-exchange chromatography. In: Carbohydrate Research. 1995 ; Vol. 270, No. 1. pp. 63-69.
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The resolution of oligosaccharides by high ph anion-exchange chromatography. / McGuire, J M ; Elliott, M A ; Elliott, H G ; Smith, K D .

In: Carbohydrate Research, Vol. 270, No. 1, 18.04.1995, p. 63-69.

Research output: Contribution to journalComment/debate

TY - JOUR

T1 - The resolution of oligosaccharides by high ph anion-exchange chromatography

AU - McGuire, J M

AU - Elliott, M A

AU - Elliott, H G

AU - Smith, K D

PY - 1995/4/18

Y1 - 1995/4/18

N2 - The natural heterogeneity of the oligosaccharide chains of glycoproteins requires that chromatography be an essential part of structural elucidation. Until recently, the rapid analysis of underivatised carbohydrates by HPLC was hampered, due to the extensive structural heterogeneity, by the absence of a chromatographic medium for satisfactory separation and by the lack of a sufficiently sensitive detection system. This problem has been overcome by the introduction of high pH anion exchange chromatography (HPAEC) with pulsed electrochemical detection (PED) which separates oligosaccharides as their oxyanions on micropellicular quaternary ammonium resins [1-10]. This technique resolves the linkage and branch isomers of neutral and anionic oligosaccharides to picomole sensitivity without pre- or post-column derivatisation. The DX-500 chromatography system is viewed as a major innovation in HPAEC-PED due to an improved sensitivity and resolution as a result of a new amperometric cell design and new digital detector signal processing. Our investigations have utilised the DX-500 in the analysis of various oligosaccharide mixtures in direct comparison with the DX-300 system that it has superceded.

AB - The natural heterogeneity of the oligosaccharide chains of glycoproteins requires that chromatography be an essential part of structural elucidation. Until recently, the rapid analysis of underivatised carbohydrates by HPLC was hampered, due to the extensive structural heterogeneity, by the absence of a chromatographic medium for satisfactory separation and by the lack of a sufficiently sensitive detection system. This problem has been overcome by the introduction of high pH anion exchange chromatography (HPAEC) with pulsed electrochemical detection (PED) which separates oligosaccharides as their oxyanions on micropellicular quaternary ammonium resins [1-10]. This technique resolves the linkage and branch isomers of neutral and anionic oligosaccharides to picomole sensitivity without pre- or post-column derivatisation. The DX-500 chromatography system is viewed as a major innovation in HPAEC-PED due to an improved sensitivity and resolution as a result of a new amperometric cell design and new digital detector signal processing. Our investigations have utilised the DX-500 in the analysis of various oligosaccharide mixtures in direct comparison with the DX-300 system that it has superceded.

KW - oligosaccharides

KW - high ph anion exchange chromatography (HPAEC)

KW - alpha(1) acid glycoprotein

KW - fetuin

KW - immunoglobulin G

KW - pulsed-amperometric detection

KW - monosaccharide analysis

KW - separation

U2 - 10.1016/0008-6215(94)00009-5

DO - 10.1016/0008-6215(94)00009-5

M3 - Comment/debate

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ER -