The potential of mass spectrometry for the global profiling of parasite metabolomes

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The strengths and limitations of existing mass spectrometry methods for metabolite detection and identification are discussed. A brief review is made of the methods available for quenching and extraction of cells or organisms prior to instrumental analysis. The techniques available for carrying out mass spectrometry-based profiling of metabolomes are discussed using the analysis of extracts from trypanosomes to illustrate various points regarding methods of separation and mass spectrometric analysis. The advantages of hydrophilic interaction chromatography (HILIC) for the analysis of polar metabolites are discussed. The challenges of data processing are outlined and illustrated using the example of ThermoFisher's Sieve software. The existing literature on applications of mass spectrometry to the profiling of parasite metabolomes is reviewed.
LanguageEnglish
Pages1409-1423
Number of pages15
JournalParasitology
Volume137
Issue number9
DOIs
Publication statusPublished - Aug 2010

Fingerprint

metabolome
Metabolome
Mass Spectrometry
Parasites
mass spectrometry
parasites
Trypanosomiasis
metabolites
Hydrophobic and Hydrophilic Interactions
Chromatography
sieves
Software
methodology
organisms
extracts
cells

Keywords

  • animals
  • mass spectrometry
  • metabolome
  • metabolomics
  • parasites
  • parasitology
  • trypanosomes
  • orbitrap
  • hydrophilic interaction chromatography
  • extraction
  • GC-MS
  • global profiling

Cite this

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The potential of mass spectrometry for the global profiling of parasite metabolomes. / Watson, D G.

In: Parasitology, Vol. 137, No. 9, 08.2010, p. 1409-1423.

Research output: Contribution to journalArticle

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