The phage growth limitation system in Streptomyces coelicolor A(3)2 is a toxin/antitoxin system, comprising enzymes with DNA methyltransferase, protein kinase and ATPase activity

Paul A Hoskisson, Paul Sumby, Margaret C. M. Smith

Research output: Contribution to journalArticle

17 Citations (Scopus)
129 Downloads (Pure)

Abstract

The phage growth limitation system of Streptomyces coelicolor A3(2) is an unusual bacteriophage defence mechanism. Progeny ϕC31 phage from an initial infection are thought to be modified such that subsequent infections are attenuated in a Pgl(+) host but normal in a Pgl(-) strain. Earlier work identified four genes required for phage resistance by Pgl. Here we demonstrate that Pgl is an elaborate and novel phage restriction system that, in part, comprises a toxin/antitoxin system where PglX, a DNA methyltransferase is toxic in the absence of a functional PglZ. In addition, the ATPase activity of PglY and a protein kinase activity in PglW are shown to be essential for phage resistance by Pgl. We conclude that on infection of a Pgl(+) cell by bacteriophage ϕC31, PglW transduces a signal, probably via phosphorylation, to other Pgl proteins resulting in the activation of the DNA methyltransferase, PglX and this leads to phage restriction.

Original languageEnglish
Pages (from-to)100-109
Number of pages10
JournalVirology
Volume477
Early online date12 Jan 2015
DOIs
Publication statusPublished - Mar 2015

Keywords

  • adenosine triphosphatases
  • bacterial toxins
  • bacteriophages
  • DNA restriction enzymes
  • DNA, viral
  • host-parasite interactions
  • protein kinases
  • signal transduction
  • streptomyces coelicolor

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