Abstract
| Language | English |
|---|---|
| Pages | 151-160 |
| Number of pages | 10 |
| Journal | Chemical Science |
| Volume | 1 |
| Issue number | 2 |
| Early online date | 1 Jun 2010 |
| DOIs | |
| Publication status | Published - 1 Aug 2010 |
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Keywords
- Raman spectroscopy
- Raman scattering
- SERRS
- enzyme measurement
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The past and future of enzyme measurements using surface enhanced Raman spectroscopy. / Larmour, Iain A.; Faulds, Karen; Graham, Duncan.
In: Chemical Science, Vol. 1, No. 2, 01.08.2010, p. 151-160.Research output: Contribution to journal › Article
TY - JOUR
T1 - The past and future of enzyme measurements using surface enhanced Raman spectroscopy
AU - Larmour, Iain A.
AU - Faulds, Karen
AU - Graham, Duncan
PY - 2010/8/1
Y1 - 2010/8/1
N2 - The ability to accurately and sensitively measure the activity of specific enzymes is central to many aspects of modern chemistry and when combined with new nanoscience based approaches, offers significant opportunities for advancing other scientific disciplines. We review the development of surface enhanced resonance Raman scattering (SERRS) for the detection of enzymes, from the initial direct spectroscopy of enzymes, substrate/product and inhibitors adsorbed onto metallic colloids, to the current approach of measuring enzymatic activity by recording the SERRS spectra of a product which is only 'switched on' after enzyme activity. Developments focussed on improvements to modular masked SERRS substrates, which are unmasked by specific enzymes, are also reviewed. Finally, we set out the remaining grand challenges within the area of enzymatic analysis by SERRS which include single molecule detection, in vivo studies and increased multiplexing for screening of evolved enzyme libraries.
AB - The ability to accurately and sensitively measure the activity of specific enzymes is central to many aspects of modern chemistry and when combined with new nanoscience based approaches, offers significant opportunities for advancing other scientific disciplines. We review the development of surface enhanced resonance Raman scattering (SERRS) for the detection of enzymes, from the initial direct spectroscopy of enzymes, substrate/product and inhibitors adsorbed onto metallic colloids, to the current approach of measuring enzymatic activity by recording the SERRS spectra of a product which is only 'switched on' after enzyme activity. Developments focussed on improvements to modular masked SERRS substrates, which are unmasked by specific enzymes, are also reviewed. Finally, we set out the remaining grand challenges within the area of enzymatic analysis by SERRS which include single molecule detection, in vivo studies and increased multiplexing for screening of evolved enzyme libraries.
KW - Raman spectroscopy
KW - Raman scattering
KW - SERRS
KW - enzyme measurement
UR - http://www.scopus.com/inward/record.url?scp=79551478464&partnerID=8YFLogxK
U2 - 10.1039/C0SC00226G
DO - 10.1039/C0SC00226G
M3 - Article
VL - 1
SP - 151
EP - 160
JO - Chemical Science
T2 - Chemical Science
JF - Chemical Science
SN - 2041-6520
IS - 2
ER -