The metabolite products of chlorocholine chloride (CCC) in eggs and meat of laying hens fed 15N-CCC containing diets

S Chakeredza, R A Edrada, R Ebel, U ter Meulen, Ruangelie Edrada-Ebel

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

An experiment was conducted to evaluate the metabolic products of chlorocholine chloride (CCC) in eggs and meat of laying hens fed a diet containing (15)N-CCC. Ten brown laying hens were randomly divided into two groups of five each. One group was offered (15)N-CCC free diet while the other group received a diet with 100 ppm (15)N-CCC for 11 days. Samples of eggs and meat from the laying hens were collected. Egg yolks and albumen were separated. Meat was collected from the breast and femur. The metabolic products of CCC were measured using ion trap electrospray ionisation mass spectrometry (ion trap-ESI-MS/MS). Determination of CCC or its metabolites in eggs and meat showed that CCC was metabolised to choline. Corresponding MS/MS spectra were obtained for m/z 104 (choline) or 105 ((15)N-choline), whereas nothing was detected at m/z 122 (CCC) or 123 ((15)N-CCC). The results from this study indicate that CCC will be metabolised in tissues of laying hens.

LanguageEnglish
Pages165-172
Number of pages8
JournalJournal of Animal Physiology and Animal Nutrition
Volume90
Issue number3-4
DOIs
Publication statusPublished - Apr 2006

Fingerprint

Chlormequat
laying hens
Meat
Eggs
chlorides
meat
metabolites
Diet
diet
choline
Choline
traps
Ions
ions
Egg Yolk
Electrospray Ionization Mass Spectrometry
egg albumen
femur
egg yolk
Femur

Keywords

  • chickens
  • chlormequat
  • choline
  • consumer product safety
  • drug residues
  • eggs
  • gas chromatography-mass spectrometry
  • meat
  • nitrogen isotopes
  • plant growth regulators
  • random allocation

Cite this

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title = "The metabolite products of chlorocholine chloride (CCC) in eggs and meat of laying hens fed 15N-CCC containing diets",
abstract = "An experiment was conducted to evaluate the metabolic products of chlorocholine chloride (CCC) in eggs and meat of laying hens fed a diet containing (15)N-CCC. Ten brown laying hens were randomly divided into two groups of five each. One group was offered (15)N-CCC free diet while the other group received a diet with 100 ppm (15)N-CCC for 11 days. Samples of eggs and meat from the laying hens were collected. Egg yolks and albumen were separated. Meat was collected from the breast and femur. The metabolic products of CCC were measured using ion trap electrospray ionisation mass spectrometry (ion trap-ESI-MS/MS). Determination of CCC or its metabolites in eggs and meat showed that CCC was metabolised to choline. Corresponding MS/MS spectra were obtained for m/z 104 (choline) or 105 ((15)N-choline), whereas nothing was detected at m/z 122 (CCC) or 123 ((15)N-CCC). The results from this study indicate that CCC will be metabolised in tissues of laying hens.",
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The metabolite products of chlorocholine chloride (CCC) in eggs and meat of laying hens fed 15N-CCC containing diets. / Chakeredza, S; Edrada, R A; Ebel, R; ter Meulen, U; Edrada-Ebel, Ruangelie.

In: Journal of Animal Physiology and Animal Nutrition, Vol. 90, No. 3-4, 04.2006, p. 165-172.

Research output: Contribution to journalArticle

TY - JOUR

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AU - Chakeredza, S

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AU - Edrada-Ebel, Ruangelie

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AB - An experiment was conducted to evaluate the metabolic products of chlorocholine chloride (CCC) in eggs and meat of laying hens fed a diet containing (15)N-CCC. Ten brown laying hens were randomly divided into two groups of five each. One group was offered (15)N-CCC free diet while the other group received a diet with 100 ppm (15)N-CCC for 11 days. Samples of eggs and meat from the laying hens were collected. Egg yolks and albumen were separated. Meat was collected from the breast and femur. The metabolic products of CCC were measured using ion trap electrospray ionisation mass spectrometry (ion trap-ESI-MS/MS). Determination of CCC or its metabolites in eggs and meat showed that CCC was metabolised to choline. Corresponding MS/MS spectra were obtained for m/z 104 (choline) or 105 ((15)N-choline), whereas nothing was detected at m/z 122 (CCC) or 123 ((15)N-CCC). The results from this study indicate that CCC will be metabolised in tissues of laying hens.

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KW - plant growth regulators

KW - random allocation

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