The metabolite products of chlorocholine chloride (CCC) in eggs and meat of laying hens fed 15N-CCC containing diets

S Chakeredza, R A Edrada, R Ebel, U ter Meulen, Ruangelie Edrada-Ebel

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An experiment was conducted to evaluate the metabolic products of chlorocholine chloride (CCC) in eggs and meat of laying hens fed a diet containing (15)N-CCC. Ten brown laying hens were randomly divided into two groups of five each. One group was offered (15)N-CCC free diet while the other group received a diet with 100 ppm (15)N-CCC for 11 days. Samples of eggs and meat from the laying hens were collected. Egg yolks and albumen were separated. Meat was collected from the breast and femur. The metabolic products of CCC were measured using ion trap electrospray ionisation mass spectrometry (ion trap-ESI-MS/MS). Determination of CCC or its metabolites in eggs and meat showed that CCC was metabolised to choline. Corresponding MS/MS spectra were obtained for m/z 104 (choline) or 105 ((15)N-choline), whereas nothing was detected at m/z 122 (CCC) or 123 ((15)N-CCC). The results from this study indicate that CCC will be metabolised in tissues of laying hens.

Original languageEnglish
Pages (from-to)165-172
Number of pages8
JournalJournal of Animal Physiology and Animal Nutrition
Issue number3-4
Publication statusPublished - Apr 2006


  • chickens
  • chlormequat
  • choline
  • consumer product safety
  • drug residues
  • eggs
  • gas chromatography-mass spectrometry
  • meat
  • nitrogen isotopes
  • plant growth regulators
  • random allocation

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