The metabolism of kaempferol in primary hepatocytes cultured on collagen films and gels of different composition

M. Kataropoulou, C.J. Henderson, M.H. Grant

    Research output: Contribution to conferencePaper

    Abstract

    Metabolic activity is unstable in primary hepatocyte cultures and is influenced by the matrix composition.The effect of incorporating 20% chondroitin-6-sulphate(Ch6SO4), a glycosaminoglycan, into collagen films and gels(0.3%w/v), and crosslinking the films and gels with 1,6-diaminohexane(DAH)on the glucuronidation of kaempferol by primary rat hepatocytes cultured for 48h and 7 days was investigated. Hepatocytes isolated from male Sprague-Dawley rats by collagenase perfusion were cultured(3x106/60mm Petri dish)in Chee's medium with 5% v/v foetal calf serum. Cells were incubated with 100mM kaempferol for 1h at 378C and the glucuronides( K1 and K2)were measured by HPLC. Cells cultured on collagen films formed only the K2 metabolite after 48h in culture. The addition of Ch6SO4 or DAH significantly increased the formation of this glucuronide. However, cells seeded on gels showed no metabolism after 48h in culture. By 7 days in culture, both K1 and K2 glucuronides were formed in cells on all the different films and gels. The formation of K1 glucuronide was significantly higher with the addition of Ch6SO4 to the films. K2 glucuronide was significantly higher in all of the crosslinked films compared to the plain films. Modification of collagen based substrates may improve cultured hepatocyte phenotype.
    Original languageEnglish
    Publication statusPublished - 2002
    EventRSC-DMG 2002: New Technologies in Drug Discovery - Edinburgh, Scotland
    Duration: 12 Dec 200213 Dec 2002

    Conference

    ConferenceRSC-DMG 2002: New Technologies in Drug Discovery
    CityEdinburgh, Scotland
    Period12/12/0213/12/02

    Keywords

    • kaempferol
    • primary hepatocytes
    • collagen
    • gels
    • composites
    • biomaterials
    • bioengineering

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    Kataropoulou, M., Henderson, C. J., & Grant, M. H. (2002). The metabolism of kaempferol in primary hepatocytes cultured on collagen films and gels of different composition. Paper presented at RSC-DMG 2002: New Technologies in Drug Discovery, Edinburgh, Scotland, .