The integration of mitochondrial calcium transport and storage

David Nicholls, Susan Chalmers

Research output: Contribution to journalLiterature review

107 Citations (Scopus)

Abstract

The extraordinary capacity of isolated mitochondria to accumulate Ca(2+) has been established for more than 40 years. The distinct kinetics of the independent uptake and efflux pathways accounts for the dual functionality of the transport process to either modulate matrix free Ca(2+) concentrations or to act as temporary stores of large amounts of Ca(2+) in the presence of phosphate. One puzzle has been the nature of the matrix calcium phosphate complex, since matrix free Ca(2+) seems to be buffered in the region of 1-5 microM in the presence of phosphate while millimolar Ca(2+) remains soluble in in vitro media. The key seems to be the elevated matrix pH and the third-power relationship of the PO(4)(3-) concentration with pH. Taking this into account we may now finally have a model that explains the major features of physiological mitochondrial Ca(2+) transport.
Original languageEnglish
Article number15377857
Pages (from-to)277-281
Number of pages5
JournalJournal of Bioenergetics and Biomembranes
Volume36
Issue number4
DOIs
Publication statusPublished - Aug 2004
EventMitochondria and Neuroprotection Symposium - Ft Lauderdale, FL , United States
Duration: 16 Apr 200419 Apr 2004

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Phosphates
Calcium
Mitochondria
In Vitro Techniques
calcium phosphate

Keywords

  • mitochondria
  • hydroxyapatite
  • pH
  • tricalcium phosphate
  • membrane potential
  • phosphate
  • calcium

Cite this

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abstract = "The extraordinary capacity of isolated mitochondria to accumulate Ca(2+) has been established for more than 40 years. The distinct kinetics of the independent uptake and efflux pathways accounts for the dual functionality of the transport process to either modulate matrix free Ca(2+) concentrations or to act as temporary stores of large amounts of Ca(2+) in the presence of phosphate. One puzzle has been the nature of the matrix calcium phosphate complex, since matrix free Ca(2+) seems to be buffered in the region of 1-5 microM in the presence of phosphate while millimolar Ca(2+) remains soluble in in vitro media. The key seems to be the elevated matrix pH and the third-power relationship of the PO(4)(3-) concentration with pH. Taking this into account we may now finally have a model that explains the major features of physiological mitochondrial Ca(2+) transport.",
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The integration of mitochondrial calcium transport and storage. / Nicholls, David; Chalmers, Susan.

In: Journal of Bioenergetics and Biomembranes, Vol. 36, No. 4, 15377857, 08.2004, p. 277-281.

Research output: Contribution to journalLiterature review

TY - JOUR

T1 - The integration of mitochondrial calcium transport and storage

AU - Nicholls, David

AU - Chalmers, Susan

PY - 2004/8

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N2 - The extraordinary capacity of isolated mitochondria to accumulate Ca(2+) has been established for more than 40 years. The distinct kinetics of the independent uptake and efflux pathways accounts for the dual functionality of the transport process to either modulate matrix free Ca(2+) concentrations or to act as temporary stores of large amounts of Ca(2+) in the presence of phosphate. One puzzle has been the nature of the matrix calcium phosphate complex, since matrix free Ca(2+) seems to be buffered in the region of 1-5 microM in the presence of phosphate while millimolar Ca(2+) remains soluble in in vitro media. The key seems to be the elevated matrix pH and the third-power relationship of the PO(4)(3-) concentration with pH. Taking this into account we may now finally have a model that explains the major features of physiological mitochondrial Ca(2+) transport.

AB - The extraordinary capacity of isolated mitochondria to accumulate Ca(2+) has been established for more than 40 years. The distinct kinetics of the independent uptake and efflux pathways accounts for the dual functionality of the transport process to either modulate matrix free Ca(2+) concentrations or to act as temporary stores of large amounts of Ca(2+) in the presence of phosphate. One puzzle has been the nature of the matrix calcium phosphate complex, since matrix free Ca(2+) seems to be buffered in the region of 1-5 microM in the presence of phosphate while millimolar Ca(2+) remains soluble in in vitro media. The key seems to be the elevated matrix pH and the third-power relationship of the PO(4)(3-) concentration with pH. Taking this into account we may now finally have a model that explains the major features of physiological mitochondrial Ca(2+) transport.

KW - mitochondria

KW - hydroxyapatite

KW - pH

KW - tricalcium phosphate

KW - membrane potential

KW - phosphate

KW - calcium

U2 - 10.1023/B:JOBB.0000041753.52832.f3

DO - 10.1023/B:JOBB.0000041753.52832.f3

M3 - Literature review

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JO - Journal of Bioenergetics and Biomembranes

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