The Ins(1,4,5)P3 binding site of bovine adrenocortical microsomes: function and regulation

S Palmer, M J Wakelam

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Adrenocortical microsomes possess a single population of Ins(1,4,5)P3-specific binding sites [IC50 5.9 +/- 0.9 nM; Palmer, Hughes, Lee & Wakelam (1988) Cell. Signalling 1, 147-156]. Competition studies showed that Ins(1:2-cyclic,4,5)P3 exhibits a 21-fold lower affinity for the site than Ins(1,4,5)P3 (IC50 124 +/- 16 nM). The affinity of the binding sites for Ins(1,4,5)P3 was not influenced by the non-hydrolysable GTP analogues GTP gamma S and Gpp[NH]p or by preincubation of the binding protein with a preparation of partially purified protein kinase C in the presence of ATP and TPA (12-O-tetradecanoylphorbol 13-acetate). These observations are discussed with reference to the identify and function of the Ins(1,4,5)P3 binding site.

LanguageEnglish
Pages593-596
Number of pages4
JournalBiochemical Journal
Volume260
Issue number2
Publication statusPublished - 1 Jun 1989
Externally publishedYes

Fingerprint

Microsomes
Binding Sites
Inhibitory Concentration 50
Guanylyl Imidodiphosphate
Guanosine 5'-O-(3-Thiotriphosphate)
Tetradecanoylphorbol Acetate
Guanosine Triphosphate
Protein Kinase C
Carrier Proteins
Acetates
Adenosine Triphosphate
Population

Keywords

  • adrenal cortex
  • animals
  • binding, competitive
  • calcium channels
  • cattle
  • GTP-binding proteins
  • inositol 1,4,5-trisphosphate
  • inositol 1,4,5-trisphosphate receptors
  • inositol phosphates
  • microsomes
  • protein kinase C
  • receptors, cell surface
  • receptors, cytoplasmic and nuclear
  • sugar phosphates

Cite this

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abstract = "Adrenocortical microsomes possess a single population of Ins(1,4,5)P3-specific binding sites [IC50 5.9 +/- 0.9 nM; Palmer, Hughes, Lee & Wakelam (1988) Cell. Signalling 1, 147-156]. Competition studies showed that Ins(1:2-cyclic,4,5)P3 exhibits a 21-fold lower affinity for the site than Ins(1,4,5)P3 (IC50 124 +/- 16 nM). The affinity of the binding sites for Ins(1,4,5)P3 was not influenced by the non-hydrolysable GTP analogues GTP gamma S and Gpp[NH]p or by preincubation of the binding protein with a preparation of partially purified protein kinase C in the presence of ATP and TPA (12-O-tetradecanoylphorbol 13-acetate). These observations are discussed with reference to the identify and function of the Ins(1,4,5)P3 binding site.",
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The Ins(1,4,5)P3 binding site of bovine adrenocortical microsomes : function and regulation. / Palmer, S; Wakelam, M J.

In: Biochemical Journal, Vol. 260, No. 2, 01.06.1989, p. 593-596.

Research output: Contribution to journalArticle

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AB - Adrenocortical microsomes possess a single population of Ins(1,4,5)P3-specific binding sites [IC50 5.9 +/- 0.9 nM; Palmer, Hughes, Lee & Wakelam (1988) Cell. Signalling 1, 147-156]. Competition studies showed that Ins(1:2-cyclic,4,5)P3 exhibits a 21-fold lower affinity for the site than Ins(1,4,5)P3 (IC50 124 +/- 16 nM). The affinity of the binding sites for Ins(1,4,5)P3 was not influenced by the non-hydrolysable GTP analogues GTP gamma S and Gpp[NH]p or by preincubation of the binding protein with a preparation of partially purified protein kinase C in the presence of ATP and TPA (12-O-tetradecanoylphorbol 13-acetate). These observations are discussed with reference to the identify and function of the Ins(1,4,5)P3 binding site.

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