The influence of the choice of digestion enzyme used to prepare rat hepatocytes on xenobiotic uptake and efflux

J.A. Sinclair, C. Henderson, J.N.A. Tettey, M.H. Grant

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Isolated rat hepatocytes are widely used to assess the metabolism and toxicity of xenobiotics. The choice of digestion enzyme used to prepare the cells has been shown previously to influence their metabolic capability. This study investigates the effect of the digestion enzyme (collagenase II, collagenase A/trypsin inhibitor, or collagenase plus dispase) on the uptake of xenobiotics into, and efflux from, hepatocytes. The choice of digestion enzymes used in this study does not affect uptake of either pravastatin (an organic anion probe substrate for Oatp transporter) or metformin (an organic cation probe substrate for Oct transporter). With regard to efflux transporters, hepatocyte differentiation was better maintained when cells were isolated using collagenase II alone.
LanguageEnglish
Pages451-457
Number of pages7
JournalToxicology in Vitro
Volume27
Issue number1
Early online date8 Aug 2012
DOIs
Publication statusPublished - Feb 2013

Fingerprint

Collagenases
Xenobiotics
Rats
Digestion
Hepatocytes
Enzymes
Pravastatin
Trypsin Inhibitors
Metformin
Substrates
Metabolism
Anions
Toxicity
Cations

Keywords

  • hepatocytes
  • collagenase
  • dispase
  • trypsin inhibitor
  • xenobiotic uptake and efflux

Cite this

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abstract = "Isolated rat hepatocytes are widely used to assess the metabolism and toxicity of xenobiotics. The choice of digestion enzyme used to prepare the cells has been shown previously to influence their metabolic capability. This study investigates the effect of the digestion enzyme (collagenase II, collagenase A/trypsin inhibitor, or collagenase plus dispase) on the uptake of xenobiotics into, and efflux from, hepatocytes. The choice of digestion enzymes used in this study does not affect uptake of either pravastatin (an organic anion probe substrate for Oatp transporter) or metformin (an organic cation probe substrate for Oct transporter). With regard to efflux transporters, hepatocyte differentiation was better maintained when cells were isolated using collagenase II alone.",
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The influence of the choice of digestion enzyme used to prepare rat hepatocytes on xenobiotic uptake and efflux. / Sinclair, J.A.; Henderson, C.; Tettey, J.N.A.; Grant, M.H.

In: Toxicology in Vitro, Vol. 27, No. 1, 02.2013, p. 451-457.

Research output: Contribution to journalArticle

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KW - dispase

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