The influence of the choice of digestion enzyme used to prepare rat hepatocytes on xenobiotic uptake and efflux

J.A. Sinclair, C. Henderson, J.N.A. Tettey, M.H. Grant

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Isolated rat hepatocytes are widely used to assess the metabolism and toxicity of xenobiotics. The choice of digestion enzyme used to prepare the cells has been shown previously to influence their metabolic capability. This study investigates the effect of the digestion enzyme (collagenase II, collagenase A/trypsin inhibitor, or collagenase plus dispase) on the uptake of xenobiotics into, and efflux from, hepatocytes. The choice of digestion enzymes used in this study does not affect uptake of either pravastatin (an organic anion probe substrate for Oatp transporter) or metformin (an organic cation probe substrate for Oct transporter). With regard to efflux transporters, hepatocyte differentiation was better maintained when cells were isolated using collagenase II alone.
Original languageEnglish
Pages (from-to)451-457
Number of pages7
JournalToxicology in Vitro
Volume27
Issue number1
Early online date8 Aug 2012
DOIs
Publication statusPublished - Feb 2013

Keywords

  • hepatocytes
  • collagenase
  • dispase
  • trypsin inhibitor
  • xenobiotic uptake and efflux

Fingerprint Dive into the research topics of 'The influence of the choice of digestion enzyme used to prepare rat hepatocytes on xenobiotic uptake and efflux'. Together they form a unique fingerprint.

Cite this