The induction of protective immunity to Leishmania major in the BALB/c mouse by interleukin 4 treatment

Katharine C. Carter, Grant Gallagher, Alan J. Baillie, James Alexander

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The ability of interleukin 4 (IL4), administered subcutaneously around the cutaneous lesion in the form of hydrophilic gels, to affect the development of established Leishmania major infections in the BALB/c mouse was studied. IL4 had a therapeutic effect on L. major lesion growth compared with control mice, causing not only resolution of the parasite lesions over a period of 10 weeks but also rendering animals resistant to reinfection. Adoptive transfer of splenic T cells, obtained from IL4‐treated animals, conferred significant resistance to L. major infection in naive BALB/c mice compared with controls. These results demonstrate that IL4 is capable of not only reversing the usual (i.e. non‐curing) course of L. major infection in the BALB/c mouse, but also of promoting the generation of protective immunity. The nature of the gel used to deliver the cytokine was found to be important since the therapeutic activity of IL4 in poloxamer gel was not observed when a hydroxypropylmethyl cellulose (hypromellose) gel was used as the IL4 vehicle.
LanguageEnglish
Pages779-782
Number of pages4
JournalEuropean Journal of Immunology
Volume19
Issue number4
DOIs
Publication statusPublished - 1 Apr 1989

Fingerprint

Leishmania major
Interleukin-4
Immunity
Gels
Therapeutics
Infection
Poloxamer
Adoptive Transfer
Therapeutic Uses
Cellulose
Parasites
Cytokines
T-Lymphocytes
Skin
Growth

Keywords

  • protective immunity
  • Leishmania major
  • interleukin 4
  • cutaneous lesion
  • mouse

Cite this

Carter, Katharine C. ; Gallagher, Grant ; Baillie, Alan J. ; Alexander , James . / The induction of protective immunity to Leishmania major in the BALB/c mouse by interleukin 4 treatment. In: European Journal of Immunology. 1989 ; Vol. 19, No. 4. pp. 779-782.
@article{106cc10f63b344a0b11fa54c35748fa0,
title = "The induction of protective immunity to Leishmania major in the BALB/c mouse by interleukin 4 treatment",
abstract = "The ability of interleukin 4 (IL4), administered subcutaneously around the cutaneous lesion in the form of hydrophilic gels, to affect the development of established Leishmania major infections in the BALB/c mouse was studied. IL4 had a therapeutic effect on L. major lesion growth compared with control mice, causing not only resolution of the parasite lesions over a period of 10 weeks but also rendering animals resistant to reinfection. Adoptive transfer of splenic T cells, obtained from IL4‐treated animals, conferred significant resistance to L. major infection in naive BALB/c mice compared with controls. These results demonstrate that IL4 is capable of not only reversing the usual (i.e. non‐curing) course of L. major infection in the BALB/c mouse, but also of promoting the generation of protective immunity. The nature of the gel used to deliver the cytokine was found to be important since the therapeutic activity of IL4 in poloxamer gel was not observed when a hydroxypropylmethyl cellulose (hypromellose) gel was used as the IL4 vehicle.",
keywords = "protective immunity, Leishmania major, interleukin 4, cutaneous lesion, mouse",
author = "Carter, {Katharine C.} and Grant Gallagher and Baillie, {Alan J.} and James Alexander",
year = "1989",
month = "4",
day = "1",
doi = "10.1002/eji.1830190432",
language = "English",
volume = "19",
pages = "779--782",
journal = "European Journal of Immunology",
issn = "0014-2980",
number = "4",

}

The induction of protective immunity to Leishmania major in the BALB/c mouse by interleukin 4 treatment. / Carter, Katharine C.; Gallagher, Grant; Baillie, Alan J.; Alexander , James .

In: European Journal of Immunology, Vol. 19, No. 4, 01.04.1989, p. 779-782.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The induction of protective immunity to Leishmania major in the BALB/c mouse by interleukin 4 treatment

AU - Carter, Katharine C.

AU - Gallagher, Grant

AU - Baillie, Alan J.

AU - Alexander , James

PY - 1989/4/1

Y1 - 1989/4/1

N2 - The ability of interleukin 4 (IL4), administered subcutaneously around the cutaneous lesion in the form of hydrophilic gels, to affect the development of established Leishmania major infections in the BALB/c mouse was studied. IL4 had a therapeutic effect on L. major lesion growth compared with control mice, causing not only resolution of the parasite lesions over a period of 10 weeks but also rendering animals resistant to reinfection. Adoptive transfer of splenic T cells, obtained from IL4‐treated animals, conferred significant resistance to L. major infection in naive BALB/c mice compared with controls. These results demonstrate that IL4 is capable of not only reversing the usual (i.e. non‐curing) course of L. major infection in the BALB/c mouse, but also of promoting the generation of protective immunity. The nature of the gel used to deliver the cytokine was found to be important since the therapeutic activity of IL4 in poloxamer gel was not observed when a hydroxypropylmethyl cellulose (hypromellose) gel was used as the IL4 vehicle.

AB - The ability of interleukin 4 (IL4), administered subcutaneously around the cutaneous lesion in the form of hydrophilic gels, to affect the development of established Leishmania major infections in the BALB/c mouse was studied. IL4 had a therapeutic effect on L. major lesion growth compared with control mice, causing not only resolution of the parasite lesions over a period of 10 weeks but also rendering animals resistant to reinfection. Adoptive transfer of splenic T cells, obtained from IL4‐treated animals, conferred significant resistance to L. major infection in naive BALB/c mice compared with controls. These results demonstrate that IL4 is capable of not only reversing the usual (i.e. non‐curing) course of L. major infection in the BALB/c mouse, but also of promoting the generation of protective immunity. The nature of the gel used to deliver the cytokine was found to be important since the therapeutic activity of IL4 in poloxamer gel was not observed when a hydroxypropylmethyl cellulose (hypromellose) gel was used as the IL4 vehicle.

KW - protective immunity

KW - Leishmania major

KW - interleukin 4

KW - cutaneous lesion

KW - mouse

U2 - 10.1002/eji.1830190432

DO - 10.1002/eji.1830190432

M3 - Article

VL - 19

SP - 779

EP - 782

JO - European Journal of Immunology

T2 - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 4

ER -