The in vitro adsorption of cytokines by polymer-pyrolysed carbon

C.A. Howell, S.R. Sandeman, G.J. Phillips, A.W. Lloyd, J.A. Davies, S.V. Mikhalovsky, S.R. Tennison, A.P. Rawlinson, O.P. Kozynchenko, H.L.H. Owen, J.D.S. Gaylor, J.J. Rouse, J.M. Courtney

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

This study investigated a range of phenolformaldehydeaniline based pyrolysed carbon matrices and their component materials, for their ability to adsorb a range of inflammatory cytokines crucial to the progression of sepsis. The efficiency of adsorption of the target molecules from human plasma was assessed and compared to that of Adsorba® 300C, a commercially available cellulose-coated activated charcoal. Results indicate that a number of the primary carbon/resin materials demonstrate efficient adsorption of the cytokines studied here (TNF, IL-6 and IL-8), comparable to other adsorbents under clinical investigation. Our findings also illustrate that these adsorbent capabilities are retained when the primary particles are combined to form a pyrolysed carbon matrix. This capability will enable the engineering of the carbon matrix porosity allowing a blend of carbonised particle combinations to be tailored for maximum adsorption of inflammatory cytokines. The present findings support further investigation of this carbon material as a combined carbon-based filtration/adsorbent device for direct blood purification.
LanguageEnglish
Pages5286-5291
Number of pages5
JournalBiomaterials
Volume27
Issue number30
DOIs
Publication statusPublished - 2006

Fingerprint

Adsorption
Polymers
Carbon
Cytokines
Adsorbents
Charcoal
Plasma (human)
Porosity
Interleukin-8
Cellulose
Activated carbon
Purification
In Vitro Techniques
Interleukin-6
Sepsis
Blood
Resins
Equipment and Supplies
Molecules

Keywords

  • adsorption
  • carbon
  • cytokine
  • extracorporeal circulation
  • sepsis
  • bioengineering

Cite this

Howell, C. A., Sandeman, S. R., Phillips, G. J., Lloyd, A. W., Davies, J. A., Mikhalovsky, S. V., ... Courtney, J. M. (2006). The in vitro adsorption of cytokines by polymer-pyrolysed carbon. Biomaterials, 27(30), 5286-5291. https://doi.org/10.1016/j.biomaterials.2006.05.041
Howell, C.A. ; Sandeman, S.R. ; Phillips, G.J. ; Lloyd, A.W. ; Davies, J.A. ; Mikhalovsky, S.V. ; Tennison, S.R. ; Rawlinson, A.P. ; Kozynchenko, O.P. ; Owen, H.L.H. ; Gaylor, J.D.S. ; Rouse, J.J. ; Courtney, J.M. / The in vitro adsorption of cytokines by polymer-pyrolysed carbon. In: Biomaterials. 2006 ; Vol. 27, No. 30. pp. 5286-5291.
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Howell, CA, Sandeman, SR, Phillips, GJ, Lloyd, AW, Davies, JA, Mikhalovsky, SV, Tennison, SR, Rawlinson, AP, Kozynchenko, OP, Owen, HLH, Gaylor, JDS, Rouse, JJ & Courtney, JM 2006, 'The in vitro adsorption of cytokines by polymer-pyrolysed carbon' Biomaterials, vol. 27, no. 30, pp. 5286-5291. https://doi.org/10.1016/j.biomaterials.2006.05.041

The in vitro adsorption of cytokines by polymer-pyrolysed carbon. / Howell, C.A.; Sandeman, S.R.; Phillips, G.J.; Lloyd, A.W.; Davies, J.A.; Mikhalovsky, S.V.; Tennison, S.R.; Rawlinson, A.P.; Kozynchenko, O.P.; Owen, H.L.H.; Gaylor, J.D.S.; Rouse, J.J.; Courtney, J.M.

In: Biomaterials, Vol. 27, No. 30, 2006, p. 5286-5291.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The in vitro adsorption of cytokines by polymer-pyrolysed carbon

AU - Howell, C.A.

AU - Sandeman, S.R.

AU - Phillips, G.J.

AU - Lloyd, A.W.

AU - Davies, J.A.

AU - Mikhalovsky, S.V.

AU - Tennison, S.R.

AU - Rawlinson, A.P.

AU - Kozynchenko, O.P.

AU - Owen, H.L.H.

AU - Gaylor, J.D.S.

AU - Rouse, J.J.

AU - Courtney, J.M.

PY - 2006

Y1 - 2006

N2 - This study investigated a range of phenolformaldehydeaniline based pyrolysed carbon matrices and their component materials, for their ability to adsorb a range of inflammatory cytokines crucial to the progression of sepsis. The efficiency of adsorption of the target molecules from human plasma was assessed and compared to that of Adsorba® 300C, a commercially available cellulose-coated activated charcoal. Results indicate that a number of the primary carbon/resin materials demonstrate efficient adsorption of the cytokines studied here (TNF, IL-6 and IL-8), comparable to other adsorbents under clinical investigation. Our findings also illustrate that these adsorbent capabilities are retained when the primary particles are combined to form a pyrolysed carbon matrix. This capability will enable the engineering of the carbon matrix porosity allowing a blend of carbonised particle combinations to be tailored for maximum adsorption of inflammatory cytokines. The present findings support further investigation of this carbon material as a combined carbon-based filtration/adsorbent device for direct blood purification.

AB - This study investigated a range of phenolformaldehydeaniline based pyrolysed carbon matrices and their component materials, for their ability to adsorb a range of inflammatory cytokines crucial to the progression of sepsis. The efficiency of adsorption of the target molecules from human plasma was assessed and compared to that of Adsorba® 300C, a commercially available cellulose-coated activated charcoal. Results indicate that a number of the primary carbon/resin materials demonstrate efficient adsorption of the cytokines studied here (TNF, IL-6 and IL-8), comparable to other adsorbents under clinical investigation. Our findings also illustrate that these adsorbent capabilities are retained when the primary particles are combined to form a pyrolysed carbon matrix. This capability will enable the engineering of the carbon matrix porosity allowing a blend of carbonised particle combinations to be tailored for maximum adsorption of inflammatory cytokines. The present findings support further investigation of this carbon material as a combined carbon-based filtration/adsorbent device for direct blood purification.

KW - adsorption

KW - carbon

KW - cytokine

KW - extracorporeal circulation

KW - sepsis

KW - bioengineering

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U2 - 10.1016/j.biomaterials.2006.05.041

DO - 10.1016/j.biomaterials.2006.05.041

M3 - Article

VL - 27

SP - 5286

EP - 5291

JO - Biomaterials

T2 - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 30

ER -

Howell CA, Sandeman SR, Phillips GJ, Lloyd AW, Davies JA, Mikhalovsky SV et al. The in vitro adsorption of cytokines by polymer-pyrolysed carbon. Biomaterials. 2006;27(30):5286-5291. https://doi.org/10.1016/j.biomaterials.2006.05.041