The IMPROVE guidelines (ischaemia models: procedural refinements of in vivo experiments)

Nathalie Percie du Sert, Alessio Alfieri, Stuart M Allan, Hilary VO Carswell, Graeme A Deuchar, Tracy D Farr, Paul Flecknell, Lindsay Gallagher, Claire L Gibson, Michael J Haley, Malcolm R Macleod, Barry W McColl, Christopher McCabe, Anna Morancho, Lawrence DF Moon, Michael J O’Neill, Isabel Pérez de Puig, Anna Planas, C Ian Ragan, Anna RosellLisa A Roy, Kathryn O Ryder, Alba Simats, Emily S Sena, Brad A Sutherland, Mark D Tricklebank, Rebecca C Trueman, Lucy Whitfield, Raymond Wong, I Mhairi Macrae

Research output: Contribution to journalArticle

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Abstract

Most in vivo models of ischaemic stroke target the middle cerebral artery and a spectrum of stroke severities, from mild to substantial, can be achieved. This review describes opportunities to improve the in vivo modelling of ischaemic stroke and animal welfare. It provides a number of recommendations to minimise the level of severity in the most common rodent models of middle cerebral artery occlusion, while sustaining or improving the scientific outcomes. The recommendations cover basic requirements pre-surgery, selecting the most appropriate anaesthetic and analgesic regimen, as well as intraoperative and post-operative care. The aim is to provide support for researchers and animal care staff to refine their procedures and practices, and implement small incremental changes to improve the welfare of the animals used and to answer the scientific question under investigation. All recommendations are recapitulated in a summary poster (see supplementary information).
Original languageEnglish
Pages (from-to)1-30
Number of pages30
JournalJournal of Cerebral Blood Flow and Metabolism
Early online date11 Aug 2017
DOIs
Publication statusE-pub ahead of print - 11 Aug 2017

Keywords

  • 3Rs
  • animal welfare
  • guidelines
  • middle cerebral artery occlusion
  • stroke

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