The impact of different biocompatible coated cardiopulmonary bypass circuits on inflammatory response and oxidative stress

T. Gourlay, L. Shedden

    Research output: Contribution to journalLetter

    1 Citation (Scopus)

    Abstract

    Background. The use of DEHP plasticised poly-vinyl-chloride (DEHPPPVC)) in medical devices persists despite evidence suggesting that DEHP migration can be harmful. Researchers have shown that a simple surface sulfonation process can retard the migration of DEHP, which may reduce the associated inflammatory response. The present study is designed to investigate the effects of surface sulfonation on DEHP migration and blood contact activation using in-vitro and rodent models. Methods. The study was carried out in two phases; Phase 1 in which the migration rate of DEHP from DEHPPPVC and sulfonated DEHP plasticised PVC (SDEHPPPVC) was measured. In phase 2 of the study the materials were incorporated into a rat recirculation biomaterial test model and blood samples taken to assess CD11b expression on neutrophils, IL-6 and Factor XIIa. Results. The initial DEHP concentration washed from the surface after storage was 37.19 ± 1.17 mg/l in the PPVC group and 5.89 ± 0.81 mg/l in the SPPVC group (P<0.0001). The post-wash migration rate was 3.07 ± 0.32 mg/l/hour in the PPVC group, compared to 0.46 ± 0.038 mg/l/hour in the SPPVC group (P<0.0001). In phase 2 of the study CD11b expression increased by 228.9% ± 37% over the test period in the PPVC group compared to 118.3% ± 46% in the SPPVC group (p<0.01). IL-6 levels rose from 3.1 ± 1.4 pg/ml to 263 ± 26 pg/ml in the PPVC group and 2.2 ± 1.6 pg/ml to 161 ± 29 pg/ml in the SPPVC group (p<0.01). Factor FXIIa levels rose from 0.22 ± 0.13 ug/ml to 3.7 ± 0.32 ug/ml and 0.28 ± 0.09 to 2.71 ± 0.21 ug/ml in the PPVC and SPPVC groups respectively (p<0.05 at 90 minutes). Conclusions. The simple sulfonation process significantly retards the migration of DEHP and is associated with the moderation of contact activation processes.
    LanguageEnglish
    Pages427-429
    Number of pages3
    JournalPerfusion
    Volume25
    Issue number6
    DOIs
    Publication statusPublished - Nov 2010

    Fingerprint

    Diethylhexyl Phthalate
    Sulfonation
    Oxidative stress
    Cardiopulmonary Bypass
    Oxidative Stress
    Networks (circuits)
    Blood
    Chemical activation
    migration
    Group
    Biomaterials
    Polyvinyl chlorides
    Vinyl Chloride
    Rats
    activation
    Interleukin-6
    Factor XIIa
    contact
    Hematologic Tests
    Biocompatible Materials

    Keywords

    • cardiopulmonary bypass circuits
    • oxidative stress

    Cite this

    @article{d0ca5e6df9814207a76e21ded01b19d7,
    title = "The impact of different biocompatible coated cardiopulmonary bypass circuits on inflammatory response and oxidative stress",
    abstract = "Background. The use of DEHP plasticised poly-vinyl-chloride (DEHPPPVC)) in medical devices persists despite evidence suggesting that DEHP migration can be harmful. Researchers have shown that a simple surface sulfonation process can retard the migration of DEHP, which may reduce the associated inflammatory response. The present study is designed to investigate the effects of surface sulfonation on DEHP migration and blood contact activation using in-vitro and rodent models. Methods. The study was carried out in two phases; Phase 1 in which the migration rate of DEHP from DEHPPPVC and sulfonated DEHP plasticised PVC (SDEHPPPVC) was measured. In phase 2 of the study the materials were incorporated into a rat recirculation biomaterial test model and blood samples taken to assess CD11b expression on neutrophils, IL-6 and Factor XIIa. Results. The initial DEHP concentration washed from the surface after storage was 37.19 ± 1.17 mg/l in the PPVC group and 5.89 ± 0.81 mg/l in the SPPVC group (P<0.0001). The post-wash migration rate was 3.07 ± 0.32 mg/l/hour in the PPVC group, compared to 0.46 ± 0.038 mg/l/hour in the SPPVC group (P<0.0001). In phase 2 of the study CD11b expression increased by 228.9{\%} ± 37{\%} over the test period in the PPVC group compared to 118.3{\%} ± 46{\%} in the SPPVC group (p<0.01). IL-6 levels rose from 3.1 ± 1.4 pg/ml to 263 ± 26 pg/ml in the PPVC group and 2.2 ± 1.6 pg/ml to 161 ± 29 pg/ml in the SPPVC group (p<0.01). Factor FXIIa levels rose from 0.22 ± 0.13 ug/ml to 3.7 ± 0.32 ug/ml and 0.28 ± 0.09 to 2.71 ± 0.21 ug/ml in the PPVC and SPPVC groups respectively (p<0.05 at 90 minutes). Conclusions. The simple sulfonation process significantly retards the migration of DEHP and is associated with the moderation of contact activation processes.",
    keywords = "cardiopulmonary bypass circuits, oxidative stress",
    author = "T. Gourlay and L. Shedden",
    year = "2010",
    month = "11",
    doi = "10.1177/0267659110381453",
    language = "English",
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    The impact of different biocompatible coated cardiopulmonary bypass circuits on inflammatory response and oxidative stress. / Gourlay, T.; Shedden, L.

    In: Perfusion, Vol. 25, No. 6, 11.2010, p. 427-429.

    Research output: Contribution to journalLetter

    TY - JOUR

    T1 - The impact of different biocompatible coated cardiopulmonary bypass circuits on inflammatory response and oxidative stress

    AU - Gourlay, T.

    AU - Shedden, L.

    PY - 2010/11

    Y1 - 2010/11

    N2 - Background. The use of DEHP plasticised poly-vinyl-chloride (DEHPPPVC)) in medical devices persists despite evidence suggesting that DEHP migration can be harmful. Researchers have shown that a simple surface sulfonation process can retard the migration of DEHP, which may reduce the associated inflammatory response. The present study is designed to investigate the effects of surface sulfonation on DEHP migration and blood contact activation using in-vitro and rodent models. Methods. The study was carried out in two phases; Phase 1 in which the migration rate of DEHP from DEHPPPVC and sulfonated DEHP plasticised PVC (SDEHPPPVC) was measured. In phase 2 of the study the materials were incorporated into a rat recirculation biomaterial test model and blood samples taken to assess CD11b expression on neutrophils, IL-6 and Factor XIIa. Results. The initial DEHP concentration washed from the surface after storage was 37.19 ± 1.17 mg/l in the PPVC group and 5.89 ± 0.81 mg/l in the SPPVC group (P<0.0001). The post-wash migration rate was 3.07 ± 0.32 mg/l/hour in the PPVC group, compared to 0.46 ± 0.038 mg/l/hour in the SPPVC group (P<0.0001). In phase 2 of the study CD11b expression increased by 228.9% ± 37% over the test period in the PPVC group compared to 118.3% ± 46% in the SPPVC group (p<0.01). IL-6 levels rose from 3.1 ± 1.4 pg/ml to 263 ± 26 pg/ml in the PPVC group and 2.2 ± 1.6 pg/ml to 161 ± 29 pg/ml in the SPPVC group (p<0.01). Factor FXIIa levels rose from 0.22 ± 0.13 ug/ml to 3.7 ± 0.32 ug/ml and 0.28 ± 0.09 to 2.71 ± 0.21 ug/ml in the PPVC and SPPVC groups respectively (p<0.05 at 90 minutes). Conclusions. The simple sulfonation process significantly retards the migration of DEHP and is associated with the moderation of contact activation processes.

    AB - Background. The use of DEHP plasticised poly-vinyl-chloride (DEHPPPVC)) in medical devices persists despite evidence suggesting that DEHP migration can be harmful. Researchers have shown that a simple surface sulfonation process can retard the migration of DEHP, which may reduce the associated inflammatory response. The present study is designed to investigate the effects of surface sulfonation on DEHP migration and blood contact activation using in-vitro and rodent models. Methods. The study was carried out in two phases; Phase 1 in which the migration rate of DEHP from DEHPPPVC and sulfonated DEHP plasticised PVC (SDEHPPPVC) was measured. In phase 2 of the study the materials were incorporated into a rat recirculation biomaterial test model and blood samples taken to assess CD11b expression on neutrophils, IL-6 and Factor XIIa. Results. The initial DEHP concentration washed from the surface after storage was 37.19 ± 1.17 mg/l in the PPVC group and 5.89 ± 0.81 mg/l in the SPPVC group (P<0.0001). The post-wash migration rate was 3.07 ± 0.32 mg/l/hour in the PPVC group, compared to 0.46 ± 0.038 mg/l/hour in the SPPVC group (P<0.0001). In phase 2 of the study CD11b expression increased by 228.9% ± 37% over the test period in the PPVC group compared to 118.3% ± 46% in the SPPVC group (p<0.01). IL-6 levels rose from 3.1 ± 1.4 pg/ml to 263 ± 26 pg/ml in the PPVC group and 2.2 ± 1.6 pg/ml to 161 ± 29 pg/ml in the SPPVC group (p<0.01). Factor FXIIa levels rose from 0.22 ± 0.13 ug/ml to 3.7 ± 0.32 ug/ml and 0.28 ± 0.09 to 2.71 ± 0.21 ug/ml in the PPVC and SPPVC groups respectively (p<0.05 at 90 minutes). Conclusions. The simple sulfonation process significantly retards the migration of DEHP and is associated with the moderation of contact activation processes.

    KW - cardiopulmonary bypass circuits

    KW - oxidative stress

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    U2 - 10.1177/0267659110381453

    DO - 10.1177/0267659110381453

    M3 - Letter

    VL - 25

    SP - 427

    EP - 429

    JO - Perfusion

    T2 - Perfusion

    JF - Perfusion

    SN - 0267-6591

    IS - 6

    ER -