The identification of DL-threo dihydrosphingosine and sphingosine as novel inhibitors of extracellular signal-regulated kinase signalling in airway smooth muscle

D Tolan, A M Conway, L Steele, S Pyne, N J Pyne

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We present entirely novel evidence that DL-threo dihydrosphingosine and sphingosine are inhibitors of the extracellular signal-regulated kinase (ERK) signalling cassette in mammalian cells. We show that DL-threo dihydrosphingosine is effective against both growth factor- and G-protein-dependent activation of ERK. We conclude that DL-threo dihydrosphingosine may represent an important pharmacological cell-permeable agent that may be usefully employed to block smooth muscle cell proliferation.
LanguageEnglish
Pages185-186
Number of pages2
JournalBritish Journal of Pharmacology
Volume119
Issue number2
Publication statusPublished - Sep 1996

Fingerprint

Sphingosine
Extracellular Signal-Regulated MAP Kinases
Smooth Muscle
GTP-Binding Proteins
Smooth Muscle Myocytes
Intercellular Signaling Peptides and Proteins
Cell Proliferation
Pharmacology
safingol

Keywords

  • animals
  • bradykinin
  • calcium-calmodulin-dependent protein kinases
  • cells, cultured
  • enzyme activation
  • enzyme inhibitors
  • guinea pigs
  • mitogen-activated protein kinase 1
  • mitogen-activated protein kinase 3
  • mitogen-activated protein kinases
  • muscle, smooth
  • platelet-derived growth factor
  • signal transduction
  • sphingosine
  • stimulation, chemical
  • trachea

Cite this

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title = "The identification of DL-threo dihydrosphingosine and sphingosine as novel inhibitors of extracellular signal-regulated kinase signalling in airway smooth muscle",
abstract = "We present entirely novel evidence that DL-threo dihydrosphingosine and sphingosine are inhibitors of the extracellular signal-regulated kinase (ERK) signalling cassette in mammalian cells. We show that DL-threo dihydrosphingosine is effective against both growth factor- and G-protein-dependent activation of ERK. We conclude that DL-threo dihydrosphingosine may represent an important pharmacological cell-permeable agent that may be usefully employed to block smooth muscle cell proliferation.",
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author = "D Tolan and Conway, {A M} and L Steele and S Pyne and Pyne, {N J}",
year = "1996",
month = "9",
language = "English",
volume = "119",
pages = "185--186",
journal = "British Journal of Pharmacology",
issn = "0007-1188",
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TY - JOUR

T1 - The identification of DL-threo dihydrosphingosine and sphingosine as novel inhibitors of extracellular signal-regulated kinase signalling in airway smooth muscle

AU - Tolan, D

AU - Conway, A M

AU - Steele, L

AU - Pyne, S

AU - Pyne, N J

PY - 1996/9

Y1 - 1996/9

N2 - We present entirely novel evidence that DL-threo dihydrosphingosine and sphingosine are inhibitors of the extracellular signal-regulated kinase (ERK) signalling cassette in mammalian cells. We show that DL-threo dihydrosphingosine is effective against both growth factor- and G-protein-dependent activation of ERK. We conclude that DL-threo dihydrosphingosine may represent an important pharmacological cell-permeable agent that may be usefully employed to block smooth muscle cell proliferation.

AB - We present entirely novel evidence that DL-threo dihydrosphingosine and sphingosine are inhibitors of the extracellular signal-regulated kinase (ERK) signalling cassette in mammalian cells. We show that DL-threo dihydrosphingosine is effective against both growth factor- and G-protein-dependent activation of ERK. We conclude that DL-threo dihydrosphingosine may represent an important pharmacological cell-permeable agent that may be usefully employed to block smooth muscle cell proliferation.

KW - animals

KW - bradykinin

KW - calcium-calmodulin-dependent protein kinases

KW - cells, cultured

KW - enzyme activation

KW - enzyme inhibitors

KW - guinea pigs

KW - mitogen-activated protein kinase 1

KW - mitogen-activated protein kinase 3

KW - mitogen-activated protein kinases

KW - muscle, smooth

KW - platelet-derived growth factor

KW - signal transduction

KW - sphingosine

KW - stimulation, chemical

KW - trachea

M3 - Article

VL - 119

SP - 185

EP - 186

JO - British Journal of Pharmacology

T2 - British Journal of Pharmacology

JF - British Journal of Pharmacology

SN - 0007-1188

IS - 2

ER -