The hydrophilic interaction like properties of some reversed phase high performance liquid chromatography columns in the analysis of basic compounds

Naser F Al-Tannak, Saud Bawazeer, Tahir H Siddiqui, David G Watson

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The hydrophilic interaction chromatography (HILIC) like properties of an ACE cyano (CN) HPLC column was studied for the separation of some basic compounds. Good separation of a test mix of basic compounds was obtained with a mobile phase consisting of acetonitrile/water (95:5) containing 3.25 mM ammonium acetate. The retention times of the basic compounds decreased with increased ionic strength or with increased water content in the mobile phase. When Trishydroxymethyl aminomethane (Tris) (pK(a) 8.0), which is a weaker amine than ammonia (pK(a) 9.3), was used as an additive in the mobile phase retention of the basic compounds was increased. The ACE CN column gave excellent peak shapes for all the basic compounds. The utility of the column for impurity profiling of two basic drugs was tested and some impurities in oxprenolol were characterised by interfacing with Fourier transform mass spectrometry. It was also observed that ACE butyl and ACE phenyl columns retained basic compounds when the columns were eluted with a mobile phase consisting of acetonitrile/water (95:5) containing 3.25 mM ammonium acetate.
LanguageEnglish
Pages1486-91
Number of pages6
JournalJournal of Chromatography A
Volume1218
Issue number11
DOIs
Publication statusPublished - 2011

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High performance liquid chromatography
Reverse-Phase Chromatography
Hydrophobic and Hydrophilic Interactions
High Pressure Liquid Chromatography
Oxprenolol
Impurities
Water
Chromatography
Ionic strength
Ammonia
Water content
Amines
Mass spectrometry
Fourier transforms
Fourier Analysis
Osmolar Concentration
Mass Spectrometry
Pharmaceutical Preparations
ammonium acetate
acetonitrile

Keywords

  • acetates
  • acetonitriles
  • chromatography, high pressure liquid
  • chromatography, Reverse-Phase
  • hydrophobic and hydrophilic Interactions
  • mass spectrometry

Cite this

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title = "The hydrophilic interaction like properties of some reversed phase high performance liquid chromatography columns in the analysis of basic compounds",
abstract = "The hydrophilic interaction chromatography (HILIC) like properties of an ACE cyano (CN) HPLC column was studied for the separation of some basic compounds. Good separation of a test mix of basic compounds was obtained with a mobile phase consisting of acetonitrile/water (95:5) containing 3.25 mM ammonium acetate. The retention times of the basic compounds decreased with increased ionic strength or with increased water content in the mobile phase. When Trishydroxymethyl aminomethane (Tris) (pK(a) 8.0), which is a weaker amine than ammonia (pK(a) 9.3), was used as an additive in the mobile phase retention of the basic compounds was increased. The ACE CN column gave excellent peak shapes for all the basic compounds. The utility of the column for impurity profiling of two basic drugs was tested and some impurities in oxprenolol were characterised by interfacing with Fourier transform mass spectrometry. It was also observed that ACE butyl and ACE phenyl columns retained basic compounds when the columns were eluted with a mobile phase consisting of acetonitrile/water (95:5) containing 3.25 mM ammonium acetate.",
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The hydrophilic interaction like properties of some reversed phase high performance liquid chromatography columns in the analysis of basic compounds. / Al-Tannak, Naser F; Bawazeer, Saud; Siddiqui, Tahir H; Watson, David G.

In: Journal of Chromatography A, Vol. 1218, No. 11, 2011, p. 1486-91.

Research output: Contribution to journalArticle

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AU - Al-Tannak, Naser F

AU - Bawazeer, Saud

AU - Siddiqui, Tahir H

AU - Watson, David G

N1 - Copyright © 2011 Elsevier B.V. All rights reserved.

PY - 2011

Y1 - 2011

N2 - The hydrophilic interaction chromatography (HILIC) like properties of an ACE cyano (CN) HPLC column was studied for the separation of some basic compounds. Good separation of a test mix of basic compounds was obtained with a mobile phase consisting of acetonitrile/water (95:5) containing 3.25 mM ammonium acetate. The retention times of the basic compounds decreased with increased ionic strength or with increased water content in the mobile phase. When Trishydroxymethyl aminomethane (Tris) (pK(a) 8.0), which is a weaker amine than ammonia (pK(a) 9.3), was used as an additive in the mobile phase retention of the basic compounds was increased. The ACE CN column gave excellent peak shapes for all the basic compounds. The utility of the column for impurity profiling of two basic drugs was tested and some impurities in oxprenolol were characterised by interfacing with Fourier transform mass spectrometry. It was also observed that ACE butyl and ACE phenyl columns retained basic compounds when the columns were eluted with a mobile phase consisting of acetonitrile/water (95:5) containing 3.25 mM ammonium acetate.

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KW - acetates

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KW - chromatography, Reverse-Phase

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