The heterogeneity of the glycosylation of alpha-1-acid glycoprotein between the sera and synovial fluid in rheumatoid arthritis

Kevin D. Smith, Aileen Pollacchi, Max Field, John Watson

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Alpha-1-acid glycoprotein (AGP) is a plasma glycoprotein produced by the liver that undergoes increased production and altered glycosylation in several physiological and pathological conditions including rheumatoid arthritis. To date, although present in the synovial fluid of rheumatoid arthritis patients, there has been no evidence for the separate extra-hepatic production of AGP. This study indicates that there could be a localized production of AGP in rheumatoid synovial fluid by demonstrating that the glycosylation patterns of AGP differed between the serum and synovial fluid in the same rheumatoid patient. Serum AGP was largely composed of fucosylated tri- and tetra-antennary oligosaccharide chains while the synovial fluid contained mainly bi-antennary chains that were fucosylated to a lesser extent. This structural heterogeneity of glycosylation resulted in functional diversity; serum but not synovial AGP is able to inhibit binding to the cell adhesion molecule E-selectin through expression of antigen sialyl Lewis X.
Original languageEnglish
Pages (from-to)261-266
Number of pages5
JournalBiomedical Chromatography
Volume16
Issue number4
DOIs
Publication statusPublished - Jun 2002

Fingerprint

Glycosylation
Orosomucoid
Synovial Fluid
Rheumatoid Arthritis
Glycoproteins
Fluids
Serum
Acids
E-Selectin
Liver
Cell Adhesion Molecules
Oligosaccharides
Plasmas

Keywords

  • epithelial cells
  • microheterogeneity
  • modulation
  • expression
  • binding
  • disease

Cite this

Smith, Kevin D. ; Pollacchi, Aileen ; Field, Max ; Watson, John. / The heterogeneity of the glycosylation of alpha-1-acid glycoprotein between the sera and synovial fluid in rheumatoid arthritis. In: Biomedical Chromatography. 2002 ; Vol. 16, No. 4. pp. 261-266.
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The heterogeneity of the glycosylation of alpha-1-acid glycoprotein between the sera and synovial fluid in rheumatoid arthritis. / Smith, Kevin D.; Pollacchi, Aileen; Field, Max; Watson, John.

In: Biomedical Chromatography, Vol. 16, No. 4, 06.2002, p. 261-266.

Research output: Contribution to journalArticle

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AU - Smith, Kevin D.

AU - Pollacchi, Aileen

AU - Field, Max

AU - Watson, John

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AB - Alpha-1-acid glycoprotein (AGP) is a plasma glycoprotein produced by the liver that undergoes increased production and altered glycosylation in several physiological and pathological conditions including rheumatoid arthritis. To date, although present in the synovial fluid of rheumatoid arthritis patients, there has been no evidence for the separate extra-hepatic production of AGP. This study indicates that there could be a localized production of AGP in rheumatoid synovial fluid by demonstrating that the glycosylation patterns of AGP differed between the serum and synovial fluid in the same rheumatoid patient. Serum AGP was largely composed of fucosylated tri- and tetra-antennary oligosaccharide chains while the synovial fluid contained mainly bi-antennary chains that were fucosylated to a lesser extent. This structural heterogeneity of glycosylation resulted in functional diversity; serum but not synovial AGP is able to inhibit binding to the cell adhesion molecule E-selectin through expression of antigen sialyl Lewis X.

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