The growth and differentiation of mesenchymal stem and progenitor cells cultured on aligned collagen matrices

Babette Lanfer, Friedrich P Seib, Uwe Freudenberg, Dimitar Stamov, Thomas Bley, Martin Bornhäuser, Carsten Werner

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Cell-matrix interactions are paramount for the successful repair and regeneration of damaged and diseased tissue. Since many tissues have an anisotropic architecture, it has been proposed that aligned extracellular matrix (ECM) structures in particular could guide and support the differentiation of resident mesenchymal stem and progenitor cells (MSCs). We therefore created aligned collagen type I structures using a microfluidic set-up with the aim to assess their impact on MSC growth and differentiation. In addition, we refined our aligned collagen matrices by incorporating the glycosaminoglycan (GAG) heparin to demonstrate the versatility of the applied methodology to study multiple ECM components in a single system. Our reconstituted, aligned ECM structures maintained and allowed multilineage (osteogenic/adipogenic/chondrogenic) differentiation of MSCs. Most noticeable was the observation that during osteogenesis, aligned collagen substrates choreographed ordered matrix mineralization. Likewise, myotube assembly of C2C12 cells was profoundly influenced by aligned topographic features resulting in enhanced myotube organization and length. Our results shed light on the regulation of MSCs through directional ECM structures and demonstrate the versatility of these cell culture platforms for guiding the morphogenesis of tissue types with anisotropic structures.
Original languageEnglish
Pages (from-to)5950-5958
Number of pages9
JournalBiomaterials
Volume30
Issue number30
DOIs
Publication statusPublished - Oct 2009

Fingerprint

Mesenchymal Stromal Cells
Collagen
Tissue
Growth
Cells
Extracellular Matrix
Cell growth
Collagen Type I
Glycosaminoglycans
Cell culture
Microfluidics
Heparin
Skeletal Muscle Fibers
Repair
Substrates
Morphogenesis
Osteogenesis
Cell Communication
Regeneration
Cell Differentiation

Keywords

  • multipotent mesenchymal stromal cells
  • extracellular matrix
  • collagen type I
  • microfluidic alignment
  • shear flow deposition

Cite this

Lanfer, Babette ; Seib, Friedrich P ; Freudenberg, Uwe ; Stamov, Dimitar ; Bley, Thomas ; Bornhäuser, Martin ; Werner, Carsten. / The growth and differentiation of mesenchymal stem and progenitor cells cultured on aligned collagen matrices. In: Biomaterials. 2009 ; Vol. 30, No. 30. pp. 5950-5958.
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The growth and differentiation of mesenchymal stem and progenitor cells cultured on aligned collagen matrices. / Lanfer, Babette; Seib, Friedrich P; Freudenberg, Uwe; Stamov, Dimitar; Bley, Thomas; Bornhäuser, Martin; Werner, Carsten.

In: Biomaterials, Vol. 30, No. 30, 10.2009, p. 5950-5958.

Research output: Contribution to journalArticle

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AU - Seib, Friedrich P

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