The escherichia coli SLC26 homologue YchM (DauA) is a C(4)-dicarboxylic acid transporter

Eleni Karinou, Emma Compton-Daw, Mélanie Morel, Arnaud Javelle

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The SLC26/SulP (solute carrier/sulphate transporter) proteins are a ubiquitous superfamily of secondary anion transporters. Prior studies have focused almost exclusively on eukaryotic members and bacterial members are frequently classified as sulphate transporters based on their homology with SulP proteins from plants and fungi. In this study we have examined the function and physiological role of the Escherichia coli Slc26 homologue, YchM. We show that there is a clear YchM-dependent growth defect when succinate is used as the sole carbon source. Using an in vivo succinate transport assay, we show that YchM is the sole aerobic succinate transporter active at acidic pH. We demonstrate that YchM can also transport other C(4) -dicarboxylic acids and that its substrate specificity differs from the well-characterized succinate transporter, DctA. Accordingly ychM was re-designated dauA (dicarboxylic acid uptake system A). Finally, our data suggest that DauA is a protein with transport and regulation activities. This is the first report that a SLC26/SulP protein acts as a C(4) -dicarboxylic acid transporter and an unexpected new function for a prokaryotic member of this transporter family.
LanguageEnglish
Pages623-640
Number of pages18
JournalMolecular Microbiology
Volume87
Issue number3
Early online date19 Dec 2012
DOIs
Publication statusPublished - Feb 2013

Fingerprint

Dicarboxylic Acid Transporters
Succinic Acid
Sulpiride
Escherichia coli
Dicarboxylic Acids
Sulfates
Plant Proteins
Protein Transport
Substrate Specificity
Anions
Proteins
Fungi
Carbon
Growth

Keywords

  • anion transport proteins
  • carbon
  • dicarboxylic acid transporters
  • escherichia coli
  • escherichia coli proteins
  • models, biological
  • phylogeny
  • sequence homology, amino acid
  • substrate specificity
  • succinic acid

Cite this

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title = "The escherichia coli SLC26 homologue YchM (DauA) is a C(4)-dicarboxylic acid transporter",
abstract = "The SLC26/SulP (solute carrier/sulphate transporter) proteins are a ubiquitous superfamily of secondary anion transporters. Prior studies have focused almost exclusively on eukaryotic members and bacterial members are frequently classified as sulphate transporters based on their homology with SulP proteins from plants and fungi. In this study we have examined the function and physiological role of the Escherichia coli Slc26 homologue, YchM. We show that there is a clear YchM-dependent growth defect when succinate is used as the sole carbon source. Using an in vivo succinate transport assay, we show that YchM is the sole aerobic succinate transporter active at acidic pH. We demonstrate that YchM can also transport other C(4) -dicarboxylic acids and that its substrate specificity differs from the well-characterized succinate transporter, DctA. Accordingly ychM was re-designated dauA (dicarboxylic acid uptake system A). Finally, our data suggest that DauA is a protein with transport and regulation activities. This is the first report that a SLC26/SulP protein acts as a C(4) -dicarboxylic acid transporter and an unexpected new function for a prokaryotic member of this transporter family.",
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The escherichia coli SLC26 homologue YchM (DauA) is a C(4)-dicarboxylic acid transporter. / Karinou, Eleni; Compton-Daw, Emma; Morel, Mélanie; Javelle, Arnaud.

In: Molecular Microbiology, Vol. 87, No. 3, 02.2013, p. 623-640.

Research output: Contribution to journalArticle

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