The EpiTect Methyl qPCR Assay as novel age estimation method in forensic biology

Shakhawan K. Mawlood, Lynn Dennany, Nigel Watson, Benjamin S. Pickard

Research output: Contribution to journalArticle

14 Citations (Scopus)
191 Downloads (Pure)

Abstract

Human aging is associated with epigenetic modification of the genome. DNA methylation at cytosines appears currently as the best characterised modification that occurs during the mammalian lifetime. Such methylation changes at regulatory region can provide insights to track contributor age for criminal investigation. The EpiTect Methyl II PCR system (QIAGEN) was used to compare methylation levels of CpG islands in the promoter regions of a number of age related genes, of which four successfully showed changes across the lifespan (NPTX2, KCNQ1DN, GRIA2 and TRIM58). This technique is based on the detection of remaining input genome after digestion with a methylation-sensitive restriction enzyme. This study examined DNA specimens from 80 female subjects of various ages (18-91 years) obtained from blood, using primers designed to flank the studied gene loci. The data obtained from DNA methylation quantification showed successful discrimination among volunteered ages. Overall, the difference between predicted and real age was about 11 years and absolute mean differences (AMD) was only 7.2 years error. We suggest the EpiTect system can be used as fast and simple innovative tool in future forensic age estimation.

Original languageEnglish
Pages (from-to)132-138
Number of pages7
JournalForensic Science International
Volume264
Early online date14 Apr 2016
DOIs
Publication statusPublished - 31 Jul 2016
Event7th European Academy of Forensic Science - Prague, Czech Republic
Duration: 6 Sep 201511 Sep 2015

Fingerprint

Methylation
Assays
Genes
DNA Methylation
Genome
CpG Islands
Nucleic Acid Regulatory Sequences
Cytosine
Genetic Promoter Regions
Epigenomics
Digestion
Polymerase Chain Reaction
Blood
Aging of materials
DNA
Enzymes

Keywords

  • human aging
  • DNA methylation
  • criminal investigation
  • forensic biology
  • DNA

Cite this

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title = "The EpiTect Methyl qPCR Assay as novel age estimation method in forensic biology",
abstract = "Human aging is associated with epigenetic modification of the genome. DNA methylation at cytosines appears currently as the best characterised modification that occurs during the mammalian lifetime. Such methylation changes at regulatory region can provide insights to track contributor age for criminal investigation. The EpiTect Methyl II PCR system (QIAGEN) was used to compare methylation levels of CpG islands in the promoter regions of a number of age related genes, of which four successfully showed changes across the lifespan (NPTX2, KCNQ1DN, GRIA2 and TRIM58). This technique is based on the detection of remaining input genome after digestion with a methylation-sensitive restriction enzyme. This study examined DNA specimens from 80 female subjects of various ages (18-91 years) obtained from blood, using primers designed to flank the studied gene loci. The data obtained from DNA methylation quantification showed successful discrimination among volunteered ages. Overall, the difference between predicted and real age was about 11 years and absolute mean differences (AMD) was only 7.2 years error. We suggest the EpiTect system can be used as fast and simple innovative tool in future forensic age estimation.",
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The EpiTect Methyl qPCR Assay as novel age estimation method in forensic biology. / Mawlood, Shakhawan K.; Dennany, Lynn; Watson, Nigel; Pickard, Benjamin S.

In: Forensic Science International, Vol. 264, 31.07.2016, p. 132-138.

Research output: Contribution to journalArticle

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N2 - Human aging is associated with epigenetic modification of the genome. DNA methylation at cytosines appears currently as the best characterised modification that occurs during the mammalian lifetime. Such methylation changes at regulatory region can provide insights to track contributor age for criminal investigation. The EpiTect Methyl II PCR system (QIAGEN) was used to compare methylation levels of CpG islands in the promoter regions of a number of age related genes, of which four successfully showed changes across the lifespan (NPTX2, KCNQ1DN, GRIA2 and TRIM58). This technique is based on the detection of remaining input genome after digestion with a methylation-sensitive restriction enzyme. This study examined DNA specimens from 80 female subjects of various ages (18-91 years) obtained from blood, using primers designed to flank the studied gene loci. The data obtained from DNA methylation quantification showed successful discrimination among volunteered ages. Overall, the difference between predicted and real age was about 11 years and absolute mean differences (AMD) was only 7.2 years error. We suggest the EpiTect system can be used as fast and simple innovative tool in future forensic age estimation.

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