TY - JOUR
T1 - The effect of the non-ionic surfactant brij 30 on the cytotoxicity of adriamycin in monolayer, spheroid and clonogenic culture systems
AU - Kerr, D.J.
AU - Wheldon, T.E.
AU - Russell, J.G.
AU - Maurer, H.R.
AU - Florence, A.T.
AU - Halbert, G.W.
AU - Freshney, R.I.
AU - Kaye, S.B.
PY - 1987/9/30
Y1 - 1987/9/30
N2 - The effects of a non-ionic polyoxyethylated lauryl ether surfactant (Brij 30) on monolayer uptake and spheroid penetration of adriamycin have been studied. Co-incubation of adriamycin with Brij 30 increases intracellular adriamycin levels by 2-3-fold. Although, in the concentrations used, Brij 30 alone is not cytotoxic, adriamycin and Brij 30 mixtures are significantly more cytotoxic (monlayer id90 = 0.6 μg/ml; disaggregated spheroid id30 = 1.9 μg/ml) and induce significantly longer spheroid growth delay than adriamycin alone (monolayer id90 = 2.1 μg/ml; disaggregated spheroid id50 = 3.3 μg/ml). Adriamycin is equally cytotoxic to mouse normal granulocytes and chronic myeloid leukaemic (M1 cell line) cells in agar clonogenic cultures. The addition of Brij 30 appears to enhance preferentially the activity of adriamycin against these tumour cells relative to the normal granulocytes.
AB - The effects of a non-ionic polyoxyethylated lauryl ether surfactant (Brij 30) on monolayer uptake and spheroid penetration of adriamycin have been studied. Co-incubation of adriamycin with Brij 30 increases intracellular adriamycin levels by 2-3-fold. Although, in the concentrations used, Brij 30 alone is not cytotoxic, adriamycin and Brij 30 mixtures are significantly more cytotoxic (monlayer id90 = 0.6 μg/ml; disaggregated spheroid id30 = 1.9 μg/ml) and induce significantly longer spheroid growth delay than adriamycin alone (monolayer id90 = 2.1 μg/ml; disaggregated spheroid id50 = 3.3 μg/ml). Adriamycin is equally cytotoxic to mouse normal granulocytes and chronic myeloid leukaemic (M1 cell line) cells in agar clonogenic cultures. The addition of Brij 30 appears to enhance preferentially the activity of adriamycin against these tumour cells relative to the normal granulocytes.
KW - Brij 30
KW - cytotoxic
KW - spheroid growth delay
KW - tumour cells
UR - http://www.scopus.com/inward/record.url?scp=0023489340&partnerID=8YFLogxK
U2 - 10.1016/0277-5379(87)90114-3
DO - 10.1016/0277-5379(87)90114-3
M3 - Article
C2 - 3678325
AN - SCOPUS:0023489340
SN - 0277-5379
VL - 23
JO - European Journal of Cancer and Clinical Oncology
JF - European Journal of Cancer and Clinical Oncology
IS - 9
ER -