The effect of point mutation on the equilibrium structural fluctuations of ferric Myoglobin

Katrin Adamczyk, Marco Candelaresi, Rafal Kania, Kirsty Robb, Cesar Bellota-Anton, Gregory M. Greetham, Mark R. Pollard, Michael Towrie, Anthony W. Parker, Paul A. Hoskisson, Nicholas P. Tucker, Neil T. Hunt

Research output: Contribution to journalArticle

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Abstract

The ultrafast equilibrium fluctuations of the FeIII-NO complex of a single point mutation of Myoglobin (H64Q) have been studied using Fourier Transform 2D-IR spectroscopy. Comparison with data from wild type Myoglobin (wt-Mb) shows the presence of two
conformational substates of the mutant haem pocket where only one exists in the wild type form. One of the substates of the mutant exhibits an almost identical NO stretching frequency and spectral diffusion dynamics to wt-Mb while the other is distinctly different in both respects. The remarkably contrasting dynamics are largely attributable to interactions
between the NO ligand and a nearby distal side chain which provides a basis for understanding the roles of these side chains in other ferric haem proteins.

LanguageEnglish
Pages7411-7419
Number of pages9
JournalPhysical Chemistry Chemical Physics
Volume14
Issue number20
Early online date29 Mar 2012
DOIs
Publication statusPublished - 2012

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myoglobin
Myoglobin
mutations
Heme
Stretching
Infrared spectroscopy
Fourier transforms
Ligands
proteins
ligands
spectroscopy
Proteins
interactions

Keywords

  • point mutation
  • ferric Myoglobin
  • Myoglobin
  • NO ligand

Cite this

Adamczyk, K., Candelaresi, M., Kania, R., Robb, K., Bellota-Anton, C., Greetham, G. M., ... Hunt, N. T. (2012). The effect of point mutation on the equilibrium structural fluctuations of ferric Myoglobin. Physical Chemistry Chemical Physics, 14(20), 7411-7419. https://doi.org/10.1039/c2cp23568d
Adamczyk, Katrin ; Candelaresi, Marco ; Kania, Rafal ; Robb, Kirsty ; Bellota-Anton, Cesar ; Greetham, Gregory M. ; Pollard, Mark R. ; Towrie, Michael ; Parker, Anthony W. ; Hoskisson, Paul A. ; Tucker, Nicholas P. ; Hunt, Neil T. / The effect of point mutation on the equilibrium structural fluctuations of ferric Myoglobin. In: Physical Chemistry Chemical Physics. 2012 ; Vol. 14, No. 20. pp. 7411-7419.
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Adamczyk, K, Candelaresi, M, Kania, R, Robb, K, Bellota-Anton, C, Greetham, GM, Pollard, MR, Towrie, M, Parker, AW, Hoskisson, PA, Tucker, NP & Hunt, NT 2012, 'The effect of point mutation on the equilibrium structural fluctuations of ferric Myoglobin' Physical Chemistry Chemical Physics, vol. 14, no. 20, pp. 7411-7419. https://doi.org/10.1039/c2cp23568d

The effect of point mutation on the equilibrium structural fluctuations of ferric Myoglobin. / Adamczyk, Katrin; Candelaresi, Marco; Kania, Rafal; Robb, Kirsty; Bellota-Anton, Cesar; Greetham, Gregory M.; Pollard, Mark R.; Towrie, Michael; Parker, Anthony W.; Hoskisson, Paul A.; Tucker, Nicholas P.; Hunt, Neil T.

In: Physical Chemistry Chemical Physics, Vol. 14, No. 20, 2012, p. 7411-7419.

Research output: Contribution to journalArticle

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AU - Adamczyk, Katrin

AU - Candelaresi, Marco

AU - Kania, Rafal

AU - Robb, Kirsty

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AU - Greetham, Gregory M.

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AU - Towrie, Michael

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AU - Hoskisson, Paul A.

AU - Tucker, Nicholas P.

AU - Hunt, Neil T.

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