The effect of methanol washing of plasticized polyvinyl chloride on biomaterial-contact-mediated CD11b (mac-1) expression in a rat recirculation model

T. Gourlay, D.C. Stefanou, K.M. Taylor

    Research output: Contribution to journalArticle

    11 Citations (Scopus)

    Abstract

    Our objective was to assess whether using a methanol wash to reduce the level of plasticizer present on the surface of medical-grade polyvinyl chloride (PVC) has a moderating effect on the expression of CD11b (mac-1) on neutrophils in rats undergoing recirculation. The study was carried out on 3 groups of 10 adult male Sprague-Dawley rats weighing between 350 and 450 g. In the 2 test groups, the animals were exposed to 48 cm2 of di-(2-ethyl-hexyl)-phthalate (DEHP)-plasticized PVC in a parallel plate recirculating test cell through which blood was recirculated at 1.5 ml/min. In the first test group, the PVC was untreated; in the second test group, the PVC was washed in methanol to reduce the level of plasticizer on the surface. The test cell was connected to the right femoral circulation, and recirculation was established for a period of 60 min. Blood samples were taken at 0, 30, and 60 min for assessment of CD11b expression on neutrophils using flow cytometric analysis. In a third group of 10 control experiments, rats underwent the entire surgical procedure, but without recirculation through the test cell. There was statistically significant (p < 0.001) lower Cdllb expression on neutrophils in the blood of rats perfused through the cell containing methanol-washed PVC after 30 min and at 60 min. CD11b expression was significantly (p < 0.001) lower in the control group than in both test groups at both the 30 and 60 min time points and at the 60 min time point on comparison with the group where blood was perfused through methanol-washed PVC. These results demonstrate that the biomaterial-contact-mediated upregulation of CD11b may be significantly reduced by employing a methanol-washing technique on the plasticized PVC. Although this technique does not entirely eliminate the expression of CDllb on neutrophils, the difference is significant and suggests the role of the plasticizer in the development of this inappropriate inflammatory response.
    LanguageEnglish
    Pages5-9
    Number of pages4
    JournalArtificial Organs
    Volume26
    Issue number1
    DOIs
    Publication statusPublished - 2002

    Fingerprint

    Biocompatible Materials
    Polyvinyl Chloride
    Biomaterials
    Washing
    Polyvinyl chlorides
    Methanol
    Rats
    Plasticizers
    Neutrophils
    Blood
    Control Groups
    Weighing
    Blood Group Antigens
    Thigh
    Sprague Dawley Rats
    Animals
    Up-Regulation

    Keywords

    • CD11b expression
    • inflammatory response
    • methanol-washing
    • neutrophils
    • artificial organs
    • bioengineering
    • rat experiments

    Cite this

    @article{55e6aae8ddc8457788bf8daf32e945cb,
    title = "The effect of methanol washing of plasticized polyvinyl chloride on biomaterial-contact-mediated CD11b (mac-1) expression in a rat recirculation model",
    abstract = "Our objective was to assess whether using a methanol wash to reduce the level of plasticizer present on the surface of medical-grade polyvinyl chloride (PVC) has a moderating effect on the expression of CD11b (mac-1) on neutrophils in rats undergoing recirculation. The study was carried out on 3 groups of 10 adult male Sprague-Dawley rats weighing between 350 and 450 g. In the 2 test groups, the animals were exposed to 48 cm2 of di-(2-ethyl-hexyl)-phthalate (DEHP)-plasticized PVC in a parallel plate recirculating test cell through which blood was recirculated at 1.5 ml/min. In the first test group, the PVC was untreated; in the second test group, the PVC was washed in methanol to reduce the level of plasticizer on the surface. The test cell was connected to the right femoral circulation, and recirculation was established for a period of 60 min. Blood samples were taken at 0, 30, and 60 min for assessment of CD11b expression on neutrophils using flow cytometric analysis. In a third group of 10 control experiments, rats underwent the entire surgical procedure, but without recirculation through the test cell. There was statistically significant (p < 0.001) lower Cdllb expression on neutrophils in the blood of rats perfused through the cell containing methanol-washed PVC after 30 min and at 60 min. CD11b expression was significantly (p < 0.001) lower in the control group than in both test groups at both the 30 and 60 min time points and at the 60 min time point on comparison with the group where blood was perfused through methanol-washed PVC. These results demonstrate that the biomaterial-contact-mediated upregulation of CD11b may be significantly reduced by employing a methanol-washing technique on the plasticized PVC. Although this technique does not entirely eliminate the expression of CDllb on neutrophils, the difference is significant and suggests the role of the plasticizer in the development of this inappropriate inflammatory response.",
    keywords = "CD11b expression, inflammatory response, methanol-washing, neutrophils, artificial organs, bioengineering, rat experiments",
    author = "T. Gourlay and D.C. Stefanou and K.M. Taylor",
    year = "2002",
    doi = "10.1046/j.1525-1594.2002.06730.x",
    language = "English",
    volume = "26",
    pages = "5--9",
    journal = "Artificial Organs",
    issn = "0160-564X",
    number = "1",

    }

    The effect of methanol washing of plasticized polyvinyl chloride on biomaterial-contact-mediated CD11b (mac-1) expression in a rat recirculation model. / Gourlay, T.; Stefanou, D.C.; Taylor, K.M.

    In: Artificial Organs, Vol. 26, No. 1, 2002, p. 5-9.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - The effect of methanol washing of plasticized polyvinyl chloride on biomaterial-contact-mediated CD11b (mac-1) expression in a rat recirculation model

    AU - Gourlay, T.

    AU - Stefanou, D.C.

    AU - Taylor, K.M.

    PY - 2002

    Y1 - 2002

    N2 - Our objective was to assess whether using a methanol wash to reduce the level of plasticizer present on the surface of medical-grade polyvinyl chloride (PVC) has a moderating effect on the expression of CD11b (mac-1) on neutrophils in rats undergoing recirculation. The study was carried out on 3 groups of 10 adult male Sprague-Dawley rats weighing between 350 and 450 g. In the 2 test groups, the animals were exposed to 48 cm2 of di-(2-ethyl-hexyl)-phthalate (DEHP)-plasticized PVC in a parallel plate recirculating test cell through which blood was recirculated at 1.5 ml/min. In the first test group, the PVC was untreated; in the second test group, the PVC was washed in methanol to reduce the level of plasticizer on the surface. The test cell was connected to the right femoral circulation, and recirculation was established for a period of 60 min. Blood samples were taken at 0, 30, and 60 min for assessment of CD11b expression on neutrophils using flow cytometric analysis. In a third group of 10 control experiments, rats underwent the entire surgical procedure, but without recirculation through the test cell. There was statistically significant (p < 0.001) lower Cdllb expression on neutrophils in the blood of rats perfused through the cell containing methanol-washed PVC after 30 min and at 60 min. CD11b expression was significantly (p < 0.001) lower in the control group than in both test groups at both the 30 and 60 min time points and at the 60 min time point on comparison with the group where blood was perfused through methanol-washed PVC. These results demonstrate that the biomaterial-contact-mediated upregulation of CD11b may be significantly reduced by employing a methanol-washing technique on the plasticized PVC. Although this technique does not entirely eliminate the expression of CDllb on neutrophils, the difference is significant and suggests the role of the plasticizer in the development of this inappropriate inflammatory response.

    AB - Our objective was to assess whether using a methanol wash to reduce the level of plasticizer present on the surface of medical-grade polyvinyl chloride (PVC) has a moderating effect on the expression of CD11b (mac-1) on neutrophils in rats undergoing recirculation. The study was carried out on 3 groups of 10 adult male Sprague-Dawley rats weighing between 350 and 450 g. In the 2 test groups, the animals were exposed to 48 cm2 of di-(2-ethyl-hexyl)-phthalate (DEHP)-plasticized PVC in a parallel plate recirculating test cell through which blood was recirculated at 1.5 ml/min. In the first test group, the PVC was untreated; in the second test group, the PVC was washed in methanol to reduce the level of plasticizer on the surface. The test cell was connected to the right femoral circulation, and recirculation was established for a period of 60 min. Blood samples were taken at 0, 30, and 60 min for assessment of CD11b expression on neutrophils using flow cytometric analysis. In a third group of 10 control experiments, rats underwent the entire surgical procedure, but without recirculation through the test cell. There was statistically significant (p < 0.001) lower Cdllb expression on neutrophils in the blood of rats perfused through the cell containing methanol-washed PVC after 30 min and at 60 min. CD11b expression was significantly (p < 0.001) lower in the control group than in both test groups at both the 30 and 60 min time points and at the 60 min time point on comparison with the group where blood was perfused through methanol-washed PVC. These results demonstrate that the biomaterial-contact-mediated upregulation of CD11b may be significantly reduced by employing a methanol-washing technique on the plasticized PVC. Although this technique does not entirely eliminate the expression of CDllb on neutrophils, the difference is significant and suggests the role of the plasticizer in the development of this inappropriate inflammatory response.

    KW - CD11b expression

    KW - inflammatory response

    KW - methanol-washing

    KW - neutrophils

    KW - artificial organs

    KW - bioengineering

    KW - rat experiments

    UR - http://dx.doi.org/10.1046/j.1525-1594.2002.06730.x

    U2 - 10.1046/j.1525-1594.2002.06730.x

    DO - 10.1046/j.1525-1594.2002.06730.x

    M3 - Article

    VL - 26

    SP - 5

    EP - 9

    JO - Artificial Organs

    T2 - Artificial Organs

    JF - Artificial Organs

    SN - 0160-564X

    IS - 1

    ER -