The effect of hypoxia on lipid phosphate receptor and sphingosine kinase expression and mitogen-activated protein kinase signaling in human pulmonary smooth muscle cells

J.S.L. Long, N.J. Pyne, S. Pyne

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Abstract

Both acute and chronic hypoxia had no effect on S1P1, S1P3 or LPA1 receptor transcript expression in human pulmonary smooth muscle cells. However, acute hypoxia increased sphingosine kinase SK1/2 and LPP1 mRNA transcript levels, while chronic hypoxia increased SK1 mRNA transcript alone. Acute hypoxia had no effect on S1P-, PDGF- or phorbol ester (PMA)-stimulated activation of ERK-1/2, but increased the ability of S1P to activate p38 MAPK. Chronic hypoxia increased the ability of S1P to stimulate the phosphorylation of ERK-1/2. Therefore, we have demonstrated for the first time that hypoxia can lead to marked changes in the expression of genes involved in S1P production and may modify post S1P receptor signal transduction pathways.
LanguageEnglish
Pages278-286
Number of pages8
JournalProstaglandins and Other Lipid Mediators
Volume79
Issue number3-4
DOIs
Publication statusPublished - May 2006

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Lysosphingolipid Receptors
Mitogen-Activated Protein Kinases
Smooth Muscle Myocytes
Muscle
Phosphates
Cells
Lipids
Lung
Lysophosphatidic Acid Receptors
Signal transduction
Messenger RNA
Phosphorylation
Phorbol Esters
p38 Mitogen-Activated Protein Kinases
Genes
Chemical activation
sphingosine kinase
Hypoxia
Signal Transduction
Gene Expression

Keywords

  • sphingosine 1-phosphate receptor
  • lysophosphatidic acid receptor
  • sphingosine kinase
  • lipid phosphate phosphatase
  • extracellular signal regulated kinase

Cite this

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title = "The effect of hypoxia on lipid phosphate receptor and sphingosine kinase expression and mitogen-activated protein kinase signaling in human pulmonary smooth muscle cells",
abstract = "Both acute and chronic hypoxia had no effect on S1P1, S1P3 or LPA1 receptor transcript expression in human pulmonary smooth muscle cells. However, acute hypoxia increased sphingosine kinase SK1/2 and LPP1 mRNA transcript levels, while chronic hypoxia increased SK1 mRNA transcript alone. Acute hypoxia had no effect on S1P-, PDGF- or phorbol ester (PMA)-stimulated activation of ERK-1/2, but increased the ability of S1P to activate p38 MAPK. Chronic hypoxia increased the ability of S1P to stimulate the phosphorylation of ERK-1/2. Therefore, we have demonstrated for the first time that hypoxia can lead to marked changes in the expression of genes involved in S1P production and may modify post S1P receptor signal transduction pathways.",
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T1 - The effect of hypoxia on lipid phosphate receptor and sphingosine kinase expression and mitogen-activated protein kinase signaling in human pulmonary smooth muscle cells

AU - Long, J.S.L.

AU - Pyne, N.J.

AU - Pyne, S.

PY - 2006/5

Y1 - 2006/5

N2 - Both acute and chronic hypoxia had no effect on S1P1, S1P3 or LPA1 receptor transcript expression in human pulmonary smooth muscle cells. However, acute hypoxia increased sphingosine kinase SK1/2 and LPP1 mRNA transcript levels, while chronic hypoxia increased SK1 mRNA transcript alone. Acute hypoxia had no effect on S1P-, PDGF- or phorbol ester (PMA)-stimulated activation of ERK-1/2, but increased the ability of S1P to activate p38 MAPK. Chronic hypoxia increased the ability of S1P to stimulate the phosphorylation of ERK-1/2. Therefore, we have demonstrated for the first time that hypoxia can lead to marked changes in the expression of genes involved in S1P production and may modify post S1P receptor signal transduction pathways.

AB - Both acute and chronic hypoxia had no effect on S1P1, S1P3 or LPA1 receptor transcript expression in human pulmonary smooth muscle cells. However, acute hypoxia increased sphingosine kinase SK1/2 and LPP1 mRNA transcript levels, while chronic hypoxia increased SK1 mRNA transcript alone. Acute hypoxia had no effect on S1P-, PDGF- or phorbol ester (PMA)-stimulated activation of ERK-1/2, but increased the ability of S1P to activate p38 MAPK. Chronic hypoxia increased the ability of S1P to stimulate the phosphorylation of ERK-1/2. Therefore, we have demonstrated for the first time that hypoxia can lead to marked changes in the expression of genes involved in S1P production and may modify post S1P receptor signal transduction pathways.

KW - sphingosine 1-phosphate receptor

KW - lysophosphatidic acid receptor

KW - sphingosine kinase

KW - lipid phosphate phosphatase

KW - extracellular signal regulated kinase

UR - http://www.sciencedirect.com/science/journal/10988823

UR - http://dx.doi.org/10.1016/j.prostaglandins.2006.03.001

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M3 - Article

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SN - 1098-8823

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