The effect of disulphide bond reduction on cholinoreceptors in cultured skeletal muscle

Alan L. Harvey, William F. Dryden

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The effects of the disulphide bond reducing agent, dithiothreitol (DTT) were tested on cultured chick embryonic skeletal muscle. Thirty minutes treatment with 10-3 M DTT reduced the depolarization produced by acetylcholine and carbachol, but enhanced the depolarization produced by neostigmine. Hexamethonium was converted from an antagonist to an agonist by DTT treatment. Depolarization by high potassium concentrations was unaffected by DTT. The effects of DTT on carbachol and hexamethonium were completely reversed after exposure to an oxidizing agent. It was concluded that the receptors in cultured skeletal muscle are affected by disulphide bond reduction similarly to adult receptors and that the relationship between the disulphide bond and the normal function of the receptor is formed early in the differentiation of muscle.

Original languageEnglish
Pages (from-to)3093-3096
Number of pages4
JournalBiochemical Pharmacology
Volume23
Issue number22
DOIs
Publication statusPublished - 15 Nov 1974

Keywords

  • 5,5' dithiobis(2 nitrobenzoic acid)
  • acetylcholine
  • carbachol
  • cholinergic receptor
  • disulfide
  • dithiothreitol
  • drug receptor
  • hexamethonium
  • hexamethonium bromide
  • neostigmine
  • neostigmine methyl sulfate
  • drug antagonism
  • drug comparison
  • drug interaction
  • in vitro study
  • tissue culture

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