The effect of dentine on fluorescence measurements of enamel lesions in vitro

C. Rousseau, S. Vaidya, S.L. Creanor, A.F. Hall, J.M. Girkin, C.J. Whitters, R. Strang, S. McHugh

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

This study aimed to determine the contribution of the underlying dentine and the enamel-dentine junction to measurements of mineral loss from enamel recorded using quantitative light fluorescence (QLF). Sixteen square blocks (5 × 5 mm) were cut from the labial surfaces of extracted bovine incisor teeth. A dental drill was used to remove dentine and the enamel-dentine junction from half of each specimen. All specimens were embedded in epoxy resin and ground to produce a smooth, flat enamel surface. Half of the enamel surface of the block, perpendicular to where the dentine had been removed, was demineralised for 72 h prior to undertaking QLF measurements from the enamel surface to compare fluorescence loss from different areas of the block. QLF readings taken from lesions with no underlying dentine or enamel-dentine junction were very similar to readings from lesions with underlying dentine. A comparison of the two data sets demonstrated a linear relationship with a gradient of 0.95 and a y intercept of -1.24 (r2 = 0.936). From these data, the underlying dentine and enamel-dentine junction did not influence mineral loss in enamel measured using QLF.
LanguageEnglish
Pages381-385
Number of pages4
JournalCaries Research
Volume36
Issue number6
DOIs
Publication statusPublished - Nov 2002

Fingerprint

Dentin
Dental Enamel
Fluorescence
Minerals
Reading
Tooth
In Vitro Techniques
Epoxy Resins
Incisor
Lip

Keywords

  • dentine demineralisation
  • enamel
  • fluorescence

Cite this

Rousseau, C., Vaidya, S., Creanor, S. L., Hall, A. F., Girkin, J. M., Whitters, C. J., ... McHugh, S. (2002). The effect of dentine on fluorescence measurements of enamel lesions in vitro. Caries Research, 36(6), 381-385. https://doi.org/10.1159/000066533
Rousseau, C. ; Vaidya, S. ; Creanor, S.L. ; Hall, A.F. ; Girkin, J.M. ; Whitters, C.J. ; Strang, R. ; McHugh, S. / The effect of dentine on fluorescence measurements of enamel lesions in vitro. In: Caries Research. 2002 ; Vol. 36, No. 6. pp. 381-385.
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Rousseau, C, Vaidya, S, Creanor, SL, Hall, AF, Girkin, JM, Whitters, CJ, Strang, R & McHugh, S 2002, 'The effect of dentine on fluorescence measurements of enamel lesions in vitro' Caries Research, vol. 36, no. 6, pp. 381-385. https://doi.org/10.1159/000066533

The effect of dentine on fluorescence measurements of enamel lesions in vitro. / Rousseau, C.; Vaidya, S.; Creanor, S.L.; Hall, A.F.; Girkin, J.M.; Whitters, C.J.; Strang, R.; McHugh, S.

In: Caries Research, Vol. 36, No. 6, 11.2002, p. 381-385.

Research output: Contribution to journalArticle

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AU - Rousseau, C.

AU - Vaidya, S.

AU - Creanor, S.L.

AU - Hall, A.F.

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AU - Whitters, C.J.

AU - Strang, R.

AU - McHugh, S.

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AB - This study aimed to determine the contribution of the underlying dentine and the enamel-dentine junction to measurements of mineral loss from enamel recorded using quantitative light fluorescence (QLF). Sixteen square blocks (5 × 5 mm) were cut from the labial surfaces of extracted bovine incisor teeth. A dental drill was used to remove dentine and the enamel-dentine junction from half of each specimen. All specimens were embedded in epoxy resin and ground to produce a smooth, flat enamel surface. Half of the enamel surface of the block, perpendicular to where the dentine had been removed, was demineralised for 72 h prior to undertaking QLF measurements from the enamel surface to compare fluorescence loss from different areas of the block. QLF readings taken from lesions with no underlying dentine or enamel-dentine junction were very similar to readings from lesions with underlying dentine. A comparison of the two data sets demonstrated a linear relationship with a gradient of 0.95 and a y intercept of -1.24 (r2 = 0.936). From these data, the underlying dentine and enamel-dentine junction did not influence mineral loss in enamel measured using QLF.

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Rousseau C, Vaidya S, Creanor SL, Hall AF, Girkin JM, Whitters CJ et al. The effect of dentine on fluorescence measurements of enamel lesions in vitro. Caries Research. 2002 Nov;36(6):381-385. https://doi.org/10.1159/000066533