The effect of dentine on fluorescence measurements of enamel lesions in vitro

C. Rousseau, S. Vaidya, S.L. Creanor, A.F. Hall, J.M. Girkin, C.J. Whitters, R. Strang, S. McHugh

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


This study aimed to determine the contribution of the underlying dentine and the enamel-dentine junction to measurements of mineral loss from enamel recorded using quantitative light fluorescence (QLF). Sixteen square blocks (5 × 5 mm) were cut from the labial surfaces of extracted bovine incisor teeth. A dental drill was used to remove dentine and the enamel-dentine junction from half of each specimen. All specimens were embedded in epoxy resin and ground to produce a smooth, flat enamel surface. Half of the enamel surface of the block, perpendicular to where the dentine had been removed, was demineralised for 72 h prior to undertaking QLF measurements from the enamel surface to compare fluorescence loss from different areas of the block. QLF readings taken from lesions with no underlying dentine or enamel-dentine junction were very similar to readings from lesions with underlying dentine. A comparison of the two data sets demonstrated a linear relationship with a gradient of 0.95 and a y intercept of -1.24 (r2 = 0.936). From these data, the underlying dentine and enamel-dentine junction did not influence mineral loss in enamel measured using QLF.
Original languageEnglish
Pages (from-to)381-385
Number of pages4
JournalCaries Research
Issue number6
Publication statusPublished - Nov 2002


  • dentine demineralisation
  • enamel
  • fluorescence

Cite this