The effect of cyanobacteria on silica precipitation at neutral pH: implications for bacterial silicification in geothermal hot springs

Nathan Yee, Vernon R. Phoenix, Kurt O. Konhauser, Liane G. Benning, F. Grant Ferris

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

In this study, we performed silica precipitation experiments with the cyanobacteria Calothrix sp. to investigate the mechanisms of silica biomineralization. Batch silica precipitation experiments were conducted at neutral pH as a function of time, Si saturation states, temperature and ferrihydrite concentrations. The experimental results show that in solutions undersaturated with respect to amorphous silica, the interaction between Si and cell surface functional groups is weak and minimal Si sorption onto cyanobacterial surfaces occurs. In solutions at high Si supersaturation states, abiotic Si polymerization is spontaneous, and at the time scales of our experiments (1-50 h) the presence of cyanobacteria had a negligible effect on silica precipitation kinetics. At lower supersaturation states, Si polymerization is slow and the presence of cyanobacteria do not promote Si-solid phase nucleation. In contrast, experiments conducted with ferrihydrite-coated cyanobacteria significantly increase the rate of Si removal, and the extent to which Si is removed increases as a function of ferrihydrite concentration. Experiments conducted with inorganic ferrihydrite colloids (without cyanobacteria) removes similar amounts of Si, suggesting that microbial surfaces play a limited role in the silica precipitation process. Therefore, in supersaturated hydrothermal waters, silica precipitation is largely nonbiogenic and cyanobacterial surfaces have a negligible effect on silica nucleation.

LanguageEnglish
Pages83-90
Number of pages8
JournalChemical Geology
Volume199
Issue number1-2
Early online date23 May 2003
DOIs
Publication statusPublished - 30 Aug 2003
Externally publishedYes

Fingerprint

Geothermal springs
Hot springs
silicification
thermal spring
Silicon Dioxide
cyanobacterium
silica
ferrihydrite
Supersaturation
supersaturation
polymerization
experiment
Experiments
nucleation
Nucleation
Polymerization
Biomineralization
biomineralization
effect
Cyanobacteria

Keywords

  • cyanobacteria
  • hot springs
  • silica precipitation

Cite this

Yee, Nathan ; Phoenix, Vernon R. ; Konhauser, Kurt O. ; Benning, Liane G. ; Ferris, F. Grant. / The effect of cyanobacteria on silica precipitation at neutral pH : implications for bacterial silicification in geothermal hot springs. In: Chemical Geology. 2003 ; Vol. 199, No. 1-2. pp. 83-90.
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abstract = "In this study, we performed silica precipitation experiments with the cyanobacteria Calothrix sp. to investigate the mechanisms of silica biomineralization. Batch silica precipitation experiments were conducted at neutral pH as a function of time, Si saturation states, temperature and ferrihydrite concentrations. The experimental results show that in solutions undersaturated with respect to amorphous silica, the interaction between Si and cell surface functional groups is weak and minimal Si sorption onto cyanobacterial surfaces occurs. In solutions at high Si supersaturation states, abiotic Si polymerization is spontaneous, and at the time scales of our experiments (1-50 h) the presence of cyanobacteria had a negligible effect on silica precipitation kinetics. At lower supersaturation states, Si polymerization is slow and the presence of cyanobacteria do not promote Si-solid phase nucleation. In contrast, experiments conducted with ferrihydrite-coated cyanobacteria significantly increase the rate of Si removal, and the extent to which Si is removed increases as a function of ferrihydrite concentration. Experiments conducted with inorganic ferrihydrite colloids (without cyanobacteria) removes similar amounts of Si, suggesting that microbial surfaces play a limited role in the silica precipitation process. Therefore, in supersaturated hydrothermal waters, silica precipitation is largely nonbiogenic and cyanobacterial surfaces have a negligible effect on silica nucleation.",
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The effect of cyanobacteria on silica precipitation at neutral pH : implications for bacterial silicification in geothermal hot springs. / Yee, Nathan; Phoenix, Vernon R.; Konhauser, Kurt O.; Benning, Liane G.; Ferris, F. Grant.

In: Chemical Geology, Vol. 199, No. 1-2, 30.08.2003, p. 83-90.

Research output: Contribution to journalArticle

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T2 - Chemical Geology

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AU - Phoenix, Vernon R.

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AU - Ferris, F. Grant

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