The effect of cicuit surface area on CD11b(mac-1) expression in a rat recirculation model

T. Gourlay, D.C. Stefanou, G. Asimakopoulos, K.M. Taylor

    Research output: Contribution to journalArticle

    19 Citations (Scopus)

    Abstract

    The aim of the study was to assess the effect of exposed surface area of diethylhexylphthalate plasticized polyvinylchloride (PVC) on the expression of the adhesion molecule CD11b(mac-1) on neutrophils and to determine whether there is any apparent advantage in the current trend in reducing circuit surface area in terms of neutrophil activation. The study was carried out using a parallel plate rodent recirculation biomaterial testing model on 4 groups of 10 adult male Sprague Dawley rats weighing between 350 and 450 g. One group comprised the control group in which there was no biomaterial exposure. In the remaining 3 groups, the animals were subjected to either high (48 cm2), intermediate (24 cm2), or low (12 cm2) biomaterial surface area exposure. The parallel plate test cell was connected to the right femoral circulation and recirculation initiated at a flow rate of 1.5 ml/min for a period of 60 min. Blood samples were taken at 0, 30, and 60 min for the assessment of CD11b expression. Cd11b was assessed using flow cytometric analysis on neutrophils. The results demonstrated that there was a surface area related effect in the upregulation of CD11b. The difference at the terminal sample point between the highest surface area group (293.95 ± 18.57%) and the low surface area group (133.80 ± 49.31%) was highly statistically significant (p < 0.001). These results demonstrate that there may be some gain in terms of reduced inflammatory response from reducing the exposed surface area of PVC in extracorporeal perfusion circuits.
    LanguageEnglish
    Pages475-479
    Number of pages4
    JournalArtificial Organs
    Volume25
    Issue number6
    DOIs
    Publication statusPublished - Dec 2001

    Fingerprint

    Biocompatible Materials
    Rats
    Neutrophils
    Biomaterials
    Neutrophil Activation
    Thigh
    Sprague Dawley Rats
    Rodentia
    Up-Regulation
    Perfusion
    Control Groups
    Networks (circuits)
    Weighing
    Animals
    Blood
    Adhesion
    Chemical activation
    Flow rate
    Molecules
    Testing

    Keywords

    • inflammatory response
    • cardiopulmonary bypass
    • miniaturized cardiopulmonary bypass circuits
    • surgery
    • bioengineering

    Cite this

    Gourlay, T. ; Stefanou, D.C. ; Asimakopoulos, G. ; Taylor, K.M. / The effect of cicuit surface area on CD11b(mac-1) expression in a rat recirculation model. In: Artificial Organs. 2001 ; Vol. 25, No. 6. pp. 475-479.
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    The effect of cicuit surface area on CD11b(mac-1) expression in a rat recirculation model. / Gourlay, T.; Stefanou, D.C.; Asimakopoulos, G.; Taylor, K.M.

    In: Artificial Organs, Vol. 25, No. 6, 12.2001, p. 475-479.

    Research output: Contribution to journalArticle

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    T1 - The effect of cicuit surface area on CD11b(mac-1) expression in a rat recirculation model

    AU - Gourlay, T.

    AU - Stefanou, D.C.

    AU - Asimakopoulos, G.

    AU - Taylor, K.M.

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    AB - The aim of the study was to assess the effect of exposed surface area of diethylhexylphthalate plasticized polyvinylchloride (PVC) on the expression of the adhesion molecule CD11b(mac-1) on neutrophils and to determine whether there is any apparent advantage in the current trend in reducing circuit surface area in terms of neutrophil activation. The study was carried out using a parallel plate rodent recirculation biomaterial testing model on 4 groups of 10 adult male Sprague Dawley rats weighing between 350 and 450 g. One group comprised the control group in which there was no biomaterial exposure. In the remaining 3 groups, the animals were subjected to either high (48 cm2), intermediate (24 cm2), or low (12 cm2) biomaterial surface area exposure. The parallel plate test cell was connected to the right femoral circulation and recirculation initiated at a flow rate of 1.5 ml/min for a period of 60 min. Blood samples were taken at 0, 30, and 60 min for the assessment of CD11b expression. Cd11b was assessed using flow cytometric analysis on neutrophils. The results demonstrated that there was a surface area related effect in the upregulation of CD11b. The difference at the terminal sample point between the highest surface area group (293.95 ± 18.57%) and the low surface area group (133.80 ± 49.31%) was highly statistically significant (p < 0.001). These results demonstrate that there may be some gain in terms of reduced inflammatory response from reducing the exposed surface area of PVC in extracorporeal perfusion circuits.

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