TY - JOUR
T1 - The development and application of high throughput cultivation technology in bioprocess development
AU - Long, Quan
AU - Liu, Xiuxia
AU - Yang, Yankun
AU - Li, Lu
AU - Harvey, Linda
AU - McNeil, Brian
AU - Bai, Zhonghu
PY - 2014/12/1
Y1 - 2014/12/1
N2 - This review focuses on recent progress in the technology of high throughput (HTP) cultivation and its increasing application in quality by design (QbD) -driven bioprocess development. Several practical HTP strategies aimed at shortening process development (PD) timelines from DNA to large scale processes involving commercially available HTP technology platforms, including microtiter plate (MTP) culture, micro-scale bioreactors, and in parallel fermentation systems, etc., are critically reviewed in detail. This discussion focuses upon the relative strengths and weaknesses or limitations of each of these platforms in this context. Emerging prototypes of micro-bioreactors reported recently, such as milliliter (mL) scale stirred tank bioreactors, and microfludics integrated micro-scale bioreactors, and their potential for practical application in QbD-driven HTP process development are also critically appraised. The overall aim of such technology is to rapidly gain process insights, and since the analytical technology deployed in HTP systems is critically important to the achievement of this aim, this rapidly developing area is discussed. Finally, general future trends are critically reviewed.
AB - This review focuses on recent progress in the technology of high throughput (HTP) cultivation and its increasing application in quality by design (QbD) -driven bioprocess development. Several practical HTP strategies aimed at shortening process development (PD) timelines from DNA to large scale processes involving commercially available HTP technology platforms, including microtiter plate (MTP) culture, micro-scale bioreactors, and in parallel fermentation systems, etc., are critically reviewed in detail. This discussion focuses upon the relative strengths and weaknesses or limitations of each of these platforms in this context. Emerging prototypes of micro-bioreactors reported recently, such as milliliter (mL) scale stirred tank bioreactors, and microfludics integrated micro-scale bioreactors, and their potential for practical application in QbD-driven HTP process development are also critically appraised. The overall aim of such technology is to rapidly gain process insights, and since the analytical technology deployed in HTP systems is critically important to the achievement of this aim, this rapidly developing area is discussed. Finally, general future trends are critically reviewed.
KW - high throughput culture
KW - micro-bioreactor
KW - process development
KW - HTP culture strategy
UR - http://www.scopus.com/inward/record.url?scp=84914117921&partnerID=8YFLogxK
U2 - 10.1016/j.jbiotec.2014.03.028
DO - 10.1016/j.jbiotec.2014.03.028
M3 - Article
C2 - 24698846
SN - 0168-1656
VL - 192
SP - 323
EP - 338
JO - Journal of Biotechnology
JF - Journal of Biotechnology
IS - PB
ER -