The development and application of high throughput cultivation technology in bioprocess development

Quan Long, Xiuxia Liu, Yankun Yang, Lu Li, Linda Harvey, Brian McNeil, Zhonghu Bai

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

This review focuses on recent progress in the technology of high throughput (HTP) cultivation and its increasing application in quality by design (QbD) -driven bioprocess development. Several practical HTP strategies aimed at shortening process development (PD) timelines from DNA to large scale processes involving commercially available HTP technology platforms, including microtiter plate (MTP) culture, micro-scale bioreactors, and in parallel fermentation systems, etc., are critically reviewed in detail. This discussion focuses upon the relative strengths and weaknesses or limitations of each of these platforms in this context. Emerging prototypes of micro-bioreactors reported recently, such as milliliter (mL) scale stirred tank bioreactors, and microfludics integrated micro-scale bioreactors, and their potential for practical application in QbD-driven HTP process development are also critically appraised. The overall aim of such technology is to rapidly gain process insights, and since the analytical technology deployed in HTP systems is critically important to the achievement of this aim, this rapidly developing area is discussed. Finally, general future trends are critically reviewed.

Original languageEnglish
Pages (from-to)323-338
Number of pages16
JournalJournal of Biotechnology
Volume192
Issue numberPB
Early online date31 Mar 2014
DOIs
Publication statusPublished - 1 Dec 2014

Keywords

  • high throughput culture
  • micro-bioreactor
  • process development
  • HTP culture strategy

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