The determination of iron as its EDTA complex in Helix aspera by hydrophilic interaction liquid chromatography coupled to Fourier transform electrospray ionisation mass spectrometry

L. Zheng, D.G. Watson, J.N.A. Tettey, C.A. Clements

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Metal complexes of Fe(III) such as Fe(III) ethylene diamine tetraacetic acid (FeETDA) have been observed to be effective molluscicides. The mechanism of toxicity of FeEDTA complex on molluscs is not clear and it is also not known if Fe(III) in the form FeEDTA is absorbed more effectively by snails than simple iron salts. Snails were fed with molluscicide pellets containing the FeEDTA complex and also with pellets containing FePO4 after 3-4 days the hearts, kidneys and dart sacs removed and analysed for Fe(III) content. Hydrophilic interaction liquid chromatography (HILIC) coupled with Fourier transform electrospray ionisation mass spectrometry (FT-ESIMS) was used to analyse the sample extracts. The method had a very wide linear range from 2 to 10,000 ng mL−1, intra- and inter-day precisions of ca. ±0.5% were observed for the analysis of extracts from snail tissues spiked with Fe(III). The limit of detection was of 0.5 ng mL−1 for a 20 μL injection. The levels of Fe(III) in tissues from snails fed Fe EDTA pellets were 10-100 times higher than the levels in snails fed FePO4 pellets. The analysis of Cu, Zn, Ca and Mn could also be carried out using the same analytical procedure.
LanguageEnglish
Pages1165-1169
Number of pages4
JournalTalanta
Volume76
Issue number5
DOIs
Publication statusPublished - Sep 2008

Fingerprint

Electrospray ionization
Liquid chromatography
Edetic Acid
Mass spectrometry
Fourier transforms
Iron
Molluscs
Tissue
Diamines
Coordination Complexes
Toxicity
Salts
Acids
ferric phosphate
ethylene

Keywords

  • feEDTA
  • Snail
  • HILIC chromatography
  • fourier transform mass spectrometry

Cite this

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abstract = "Metal complexes of Fe(III) such as Fe(III) ethylene diamine tetraacetic acid (FeETDA) have been observed to be effective molluscicides. The mechanism of toxicity of FeEDTA complex on molluscs is not clear and it is also not known if Fe(III) in the form FeEDTA is absorbed more effectively by snails than simple iron salts. Snails were fed with molluscicide pellets containing the FeEDTA complex and also with pellets containing FePO4 after 3-4 days the hearts, kidneys and dart sacs removed and analysed for Fe(III) content. Hydrophilic interaction liquid chromatography (HILIC) coupled with Fourier transform electrospray ionisation mass spectrometry (FT-ESIMS) was used to analyse the sample extracts. The method had a very wide linear range from 2 to 10,000 ng mL−1, intra- and inter-day precisions of ca. ±0.5{\%} were observed for the analysis of extracts from snail tissues spiked with Fe(III). The limit of detection was of 0.5 ng mL−1 for a 20 μL injection. The levels of Fe(III) in tissues from snails fed Fe EDTA pellets were 10-100 times higher than the levels in snails fed FePO4 pellets. The analysis of Cu, Zn, Ca and Mn could also be carried out using the same analytical procedure.",
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The determination of iron as its EDTA complex in Helix aspera by hydrophilic interaction liquid chromatography coupled to Fourier transform electrospray ionisation mass spectrometry. / Zheng, L.; Watson, D.G.; Tettey, J.N.A.; Clements, C.A.

In: Talanta, Vol. 76, No. 5, 09.2008, p. 1165-1169.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The determination of iron as its EDTA complex in Helix aspera by hydrophilic interaction liquid chromatography coupled to Fourier transform electrospray ionisation mass spectrometry

AU - Zheng, L.

AU - Watson, D.G.

AU - Tettey, J.N.A.

AU - Clements, C.A.

PY - 2008/9

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