The degradative effects of germicidal light on flexible endoscope material: the safety and suitability of UVC and 405nm light sources

Daniel Irving, M. Helen Grant, Michelle MacLean, Dimitrios Lamprou, Scott MacGregor, John Anderson

Research output: Contribution to conferencePoster

Abstract

The use of germicidal ultraviolet (UVC) light in flexible endoscope storage has been linked with material degradation, leading to device failure and increased risk to patients. 405nm germicidal light presents a possible alternative, potentially providing bacterial inactivation without material damage.
The aim of this study was to investigate the degradative effects of UVC light on flexible endoscope material, and investigate the potential use of 405nm light as
a non-damaging alternative.
Samples of flexible endoscope insertion tube material were exposed to both germicidal light sources. Material properties were monitored using Fourier Transform Infrared Spectroscopy (FTIR), Atomic Force Microscopy (AFM),
contact angle goniometry and confocal microscopy. The adhesion of
Pseudomonas aeruginosa on exposed and unexposed samples was
investigated to determine the potential impact of material damage on biofouling.
Samples exposed to UVC light showed significant changes: variations were observed in FTIR spectra indicating changes in polymer structure; average water contact angle decreased from 82.6° to 61.4°; average surface roughness increased from 2.34nm to 68.7nm, and visible cracking of the surface was observed. In contrast, no significant changes were seen in samples exposed to 405nm light. Bacterial adhesion tests showed an 86.8% increase in P. aeruginosa
adhesion on UVC-exposed samples relative to unexposed material, and no significant increase in adhesion on samples exposed to 405nm light.
UVC light can cause notable degradation of flexible endoscope material, impacting material properties and microbiological interactions. Results indicate 405nm germicidal light represents a potential safe alternative for use in flexible endoscope storage.

Conference

ConferenceThe 9th Healthcare Infection Society International Conference 2014
Abbreviated titleHIS2014
CountryFrance
CityLyon
Period16/11/1418/11/14
Internet address

Fingerprint

Endoscopy
Light sources
Adhesion
Contact angle
Fourier transform infrared spectroscopy
Materials properties
Biofouling
Degradation
Confocal microscopy
Atomic force microscopy
Surface roughness
Polymers

Keywords

  • germicidal light
  • flexible endoscope
  • UVC
  • 405nm light

Cite this

Irving, D., Grant, M. H., MacLean, M., Lamprou, D., MacGregor, S., & Anderson, J. (2014). The degradative effects of germicidal light on flexible endoscope material: the safety and suitability of UVC and 405nm light sources. Poster session presented at The 9th Healthcare Infection Society International Conference 2014, Lyon, France.
Irving, Daniel ; Grant, M. Helen ; MacLean, Michelle ; Lamprou, Dimitrios ; MacGregor, Scott ; Anderson, John. / The degradative effects of germicidal light on flexible endoscope material : the safety and suitability of UVC and 405nm light sources. Poster session presented at The 9th Healthcare Infection Society International Conference 2014, Lyon, France.
@conference{a8c79dd037bc43329f544d7f59595ec7,
title = "The degradative effects of germicidal light on flexible endoscope material: the safety and suitability of UVC and 405nm light sources",
abstract = "The use of germicidal ultraviolet (UVC) light in flexible endoscope storage has been linked with material degradation, leading to device failure and increased risk to patients. 405nm germicidal light presents a possible alternative, potentially providing bacterial inactivation without material damage.The aim of this study was to investigate the degradative effects of UVC light on flexible endoscope material, and investigate the potential use of 405nm light asa non-damaging alternative.Samples of flexible endoscope insertion tube material were exposed to both germicidal light sources. Material properties were monitored using Fourier Transform Infrared Spectroscopy (FTIR), Atomic Force Microscopy (AFM),contact angle goniometry and confocal microscopy. The adhesion ofPseudomonas aeruginosa on exposed and unexposed samples wasinvestigated to determine the potential impact of material damage on biofouling.Samples exposed to UVC light showed significant changes: variations were observed in FTIR spectra indicating changes in polymer structure; average water contact angle decreased from 82.6° to 61.4°; average surface roughness increased from 2.34nm to 68.7nm, and visible cracking of the surface was observed. In contrast, no significant changes were seen in samples exposed to 405nm light. Bacterial adhesion tests showed an 86.8{\%} increase in P. aeruginosaadhesion on UVC-exposed samples relative to unexposed material, and no significant increase in adhesion on samples exposed to 405nm light.UVC light can cause notable degradation of flexible endoscope material, impacting material properties and microbiological interactions. Results indicate 405nm germicidal light represents a potential safe alternative for use in flexible endoscope storage.",
keywords = "germicidal light, flexible endoscope, UVC, 405nm light",
author = "Daniel Irving and Grant, {M. Helen} and Michelle MacLean and Dimitrios Lamprou and Scott MacGregor and John Anderson",
year = "2014",
month = "11",
language = "English",
note = "The 9th Healthcare Infection Society International Conference 2014, HIS2014 ; Conference date: 16-11-2014 Through 18-11-2014",
url = "http://www.his.org.uk/events/his2014/#.VMJ0Kiw9WSo",

}

Irving, D, Grant, MH, MacLean, M, Lamprou, D, MacGregor, S & Anderson, J 2014, 'The degradative effects of germicidal light on flexible endoscope material: the safety and suitability of UVC and 405nm light sources' The 9th Healthcare Infection Society International Conference 2014, Lyon, France, 16/11/14 - 18/11/14, .

The degradative effects of germicidal light on flexible endoscope material : the safety and suitability of UVC and 405nm light sources. / Irving, Daniel; Grant, M. Helen; MacLean, Michelle; Lamprou, Dimitrios; MacGregor, Scott; Anderson, John.

2014. Poster session presented at The 9th Healthcare Infection Society International Conference 2014, Lyon, France.

Research output: Contribution to conferencePoster

TY - CONF

T1 - The degradative effects of germicidal light on flexible endoscope material

T2 - the safety and suitability of UVC and 405nm light sources

AU - Irving, Daniel

AU - Grant, M. Helen

AU - MacLean, Michelle

AU - Lamprou, Dimitrios

AU - MacGregor, Scott

AU - Anderson, John

PY - 2014/11

Y1 - 2014/11

N2 - The use of germicidal ultraviolet (UVC) light in flexible endoscope storage has been linked with material degradation, leading to device failure and increased risk to patients. 405nm germicidal light presents a possible alternative, potentially providing bacterial inactivation without material damage.The aim of this study was to investigate the degradative effects of UVC light on flexible endoscope material, and investigate the potential use of 405nm light asa non-damaging alternative.Samples of flexible endoscope insertion tube material were exposed to both germicidal light sources. Material properties were monitored using Fourier Transform Infrared Spectroscopy (FTIR), Atomic Force Microscopy (AFM),contact angle goniometry and confocal microscopy. The adhesion ofPseudomonas aeruginosa on exposed and unexposed samples wasinvestigated to determine the potential impact of material damage on biofouling.Samples exposed to UVC light showed significant changes: variations were observed in FTIR spectra indicating changes in polymer structure; average water contact angle decreased from 82.6° to 61.4°; average surface roughness increased from 2.34nm to 68.7nm, and visible cracking of the surface was observed. In contrast, no significant changes were seen in samples exposed to 405nm light. Bacterial adhesion tests showed an 86.8% increase in P. aeruginosaadhesion on UVC-exposed samples relative to unexposed material, and no significant increase in adhesion on samples exposed to 405nm light.UVC light can cause notable degradation of flexible endoscope material, impacting material properties and microbiological interactions. Results indicate 405nm germicidal light represents a potential safe alternative for use in flexible endoscope storage.

AB - The use of germicidal ultraviolet (UVC) light in flexible endoscope storage has been linked with material degradation, leading to device failure and increased risk to patients. 405nm germicidal light presents a possible alternative, potentially providing bacterial inactivation without material damage.The aim of this study was to investigate the degradative effects of UVC light on flexible endoscope material, and investigate the potential use of 405nm light asa non-damaging alternative.Samples of flexible endoscope insertion tube material were exposed to both germicidal light sources. Material properties were monitored using Fourier Transform Infrared Spectroscopy (FTIR), Atomic Force Microscopy (AFM),contact angle goniometry and confocal microscopy. The adhesion ofPseudomonas aeruginosa on exposed and unexposed samples wasinvestigated to determine the potential impact of material damage on biofouling.Samples exposed to UVC light showed significant changes: variations were observed in FTIR spectra indicating changes in polymer structure; average water contact angle decreased from 82.6° to 61.4°; average surface roughness increased from 2.34nm to 68.7nm, and visible cracking of the surface was observed. In contrast, no significant changes were seen in samples exposed to 405nm light. Bacterial adhesion tests showed an 86.8% increase in P. aeruginosaadhesion on UVC-exposed samples relative to unexposed material, and no significant increase in adhesion on samples exposed to 405nm light.UVC light can cause notable degradation of flexible endoscope material, impacting material properties and microbiological interactions. Results indicate 405nm germicidal light represents a potential safe alternative for use in flexible endoscope storage.

KW - germicidal light

KW - flexible endoscope

KW - UVC

KW - 405nm light

UR - http://www.his.org.uk/files/7414/1535/3959/Poster_abstracts_for_HIS_Website_Nov_2014.pdf

UR - http://www.his.org.uk/events/his2014

M3 - Poster

ER -

Irving D, Grant MH, MacLean M, Lamprou D, MacGregor S, Anderson J. The degradative effects of germicidal light on flexible endoscope material: the safety and suitability of UVC and 405nm light sources. 2014. Poster session presented at The 9th Healthcare Infection Society International Conference 2014, Lyon, France.