The cyclin-dependent kinase PITSLRE/CDK11 is required for successful autophagy

Simon Wilkinson, Daniel Croft, Jim O'Prey, Arenda Meedendorp, Margaret O’Prey, Christine Dufès, Kevin Ryan

Research output: Contribution to journalArticle

  • 17 Citations

Abstract

(Macro)autophagy is a membrane-trafficking process that serves to sequester cellular constituents in organelles termed autophagosomes, which target their degradation in the lysosome. Autophagy operates at basal levels in all cells where it serves as a homeostatic mechanism to maintain cellular integrity. The levels and cargoes of autophagy can, however, change in response to a variety of stimuli, and perturbations in autophagy are known to be involved in the aetiology of various human diseases. Autophagy must therefore be tightly controlled. We report here that the Drosophila cyclin-dependent kinase PITSLRE is a modulator of autophagy. Loss of the human PITSLRE orthologue, CDK11, initially appears to induce autophagy, but at later time points CDK11 is critically required for autophagic flux and cargo digestion. Since PITSLRE/CDK11 regulates autophagy in both Drosophila and human cells, this kinase represents a novel phylogenetically conserved component of the autophagy machinery.
LanguageEnglish
Pages1295-1301
Number of pages7
JournalAutophagy
Volume7
Issue number11
DOIs
StatePublished - Nov 2011

Fingerprint

Cyclin-Dependent Kinases
Autophagy
Drosophila
Lysosomes
Organelles
Digestion
Phosphotransferases
Membranes

Keywords

  • cyclin-dependent kinase
  • enzymes
  • pharmacology
  • (macro)autophagy

Cite this

Wilkinson, S., Croft, D., O'Prey, J., Meedendorp, A., O’Prey, M., Dufès, C., & Ryan, K. (2011). The cyclin-dependent kinase PITSLRE/CDK11 is required for successful autophagy. Autophagy, 7(11), 1295-1301. DOI: 10.4161/auto.7.11.16646
Wilkinson, Simon ; Croft, Daniel ; O'Prey, Jim ; Meedendorp, Arenda ; O’Prey, Margaret ; Dufès, Christine ; Ryan, Kevin. / The cyclin-dependent kinase PITSLRE/CDK11 is required for successful autophagy. In: Autophagy. 2011 ; Vol. 7, No. 11. pp. 1295-1301
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Wilkinson, S, Croft, D, O'Prey, J, Meedendorp, A, O’Prey, M, Dufès, C & Ryan, K 2011, 'The cyclin-dependent kinase PITSLRE/CDK11 is required for successful autophagy' Autophagy, vol. 7, no. 11, pp. 1295-1301. DOI: 10.4161/auto.7.11.16646

The cyclin-dependent kinase PITSLRE/CDK11 is required for successful autophagy. / Wilkinson, Simon; Croft, Daniel; O'Prey, Jim; Meedendorp, Arenda; O’Prey, Margaret; Dufès, Christine; Ryan, Kevin.

In: Autophagy, Vol. 7, No. 11, 11.2011, p. 1295-1301.

Research output: Contribution to journalArticle

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AU - Dufès,Christine

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Wilkinson S, Croft D, O'Prey J, Meedendorp A, O’Prey M, Dufès C et al. The cyclin-dependent kinase PITSLRE/CDK11 is required for successful autophagy. Autophagy. 2011 Nov;7(11):1295-1301. Available from, DOI: 10.4161/auto.7.11.16646