The complex degradation and metabolism of quercetin in rat hepatocyte incubations

Khaled Omar, M. Helen Grant, Catherine Henderson, David George Watson

Research output: Contribution to journalArticle

3 Citations (Scopus)
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Abstract

1. The current study demonstrated that there is still new information to be obtained on the chemical and biological transformation of the widely studied flavonoid quercetin.
2. In rat hepatocytes, 35 metabolites of quercetin were observed by using high-resolution mass spectrometry. The metabolites included glucuronides, sulfates, mixed sulfate/glucuronide metabolites and methylated versions of these metabolites.
3. Several metabolites were formed from chemical degradation products of quercetin which were found to form in Krebs–Henseleit (KH) buffer, degradants of quercetin were also formed in the buffer under the conditions used for hepatocyte incubations.
4. The degradants and metabolites of quercetin were characterized by using high-resolution MS2. It was observed that the glutathione (GSH) conjugates of quercetin formed in large amounts in ammonium bicarbonate solution although the pattern of conjugates formed was different from that observed in hepatocytes suggesting some degree on enzymatic control on GSH conjugate formation in the hepatocyte incubations.
5. GSH conjugates were not formed when GSH was included in incubations of quercetin in KH buffer alone and only small amounts of quercetin degradation occurred. Instead, GSH was extensively converted into GSSG, thus presumably reducing the levels of oxygen in the incubation thus preventing quercetin degradation.
Original languageEnglish
Pages (from-to)1074-1082
Number of pages9
JournalXenobiotica
Volume44
Issue number12
Early online date24 Jun 2014
DOIs
Publication statusPublished - 1 Dec 2014

Keywords

  • degradation
  • glutathione adducts
  • hepatocytes
  • metabolism
  • quercetin

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