The cationic peptide magainin II is antimicrobial for Burkholderia cepacia-complex strains

Joanne E. Thwaite; Suzanne Humphrey; Marc A. Fox; Victoria L. Savage; Thomas R. Laws; David O. Ulaeto; Richard W. Titball; Helen S. Atkins

doi:10.1099/jmm.0.008128-0

The cationic peptide magainin II is antimicrobial for Burkholderia cepacia-complex strains

Joanne E. Thwaite, Suzanne Humphrey, Marc A. Fox, Victoria L. Savage, Thomas R. Laws, David O. Ulaeto, Richard W. Titball, Helen S. Atkins

Strathclyde Institute Of Pharmacy And Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

This study was undertaken to determine the antibacterial activity of eight cationic antimicrobial peptides towards strains of genomovars I–V of the Burkholderia cepacia complex (Bcc) in time–kill assays. All but one of the peptides failed to show activity against the panel of test strains. The exception was magainin II, a 23 aa peptide isolated from the epidermis of the African clawed frog, Xenopus laevis, which exhibited significant bactericidal activity for Bcc genomovars most frequently associated with lung infection of patients with cystic fibrosis. In vitro studies indicated that magainin II protected a human bronchial epithelial cell line (BEAS-2B) from killing by Bcc and suggest that this peptide may have therapeutic potential against these organisms.

Original language	English
Pages (from-to)	923-929
Number of pages	7
Journal	Journal of Medical Microbiology
Volume	58
Issue number	7
DOIs	https://doi.org/10.1099/jmm.0.008128-0
Publication status	Published - 1 Jul 2009

Keywords

cationic antimicrobial peptides
Burkholderia cepacia
cystic fibrosis

Access to Document

10.1099/jmm.0.008128-0

Cite this

@article{9171959ebdaf412aa9966b13c8bcb510,

title = "The cationic peptide magainin II is antimicrobial for Burkholderia cepacia-complex strains",

abstract = "This study was undertaken to determine the antibacterial activity of eight cationic antimicrobial peptides towards strains of genomovars I–V of the Burkholderia cepacia complex (Bcc) in time–kill assays. All but one of the peptides failed to show activity against the panel of test strains. The exception was magainin II, a 23 aa peptide isolated from the epidermis of the African clawed frog, Xenopus laevis, which exhibited significant bactericidal activity for Bcc genomovars most frequently associated with lung infection of patients with cystic fibrosis. In vitro studies indicated that magainin II protected a human bronchial epithelial cell line (BEAS-2B) from killing by Bcc and suggest that this peptide may have therapeutic potential against these organisms.",

keywords = "cationic antimicrobial peptides, Burkholderia cepacia, cystic fibrosis",

author = "Thwaite, {Joanne E.} and Suzanne Humphrey and Fox, {Marc A.} and Savage, {Victoria L.} and Laws, {Thomas R.} and Ulaeto, {David O.} and Titball, {Richard W.} and Atkins, {Helen S.}",

year = "2009",

month = jul,

day = "1",

doi = "10.1099/jmm.0.008128-0",

language = "English",

volume = "58",

pages = "923--929",

journal = "Journal of Medical Microbiology",

issn = "0022-2615",

number = "7",

}

TY - JOUR

T1 - The cationic peptide magainin II is antimicrobial for Burkholderia cepacia-complex strains

AU - Thwaite, Joanne E.

AU - Humphrey, Suzanne

AU - Fox, Marc A.

AU - Savage, Victoria L.

AU - Laws, Thomas R.

AU - Ulaeto, David O.

AU - Titball, Richard W.

AU - Atkins, Helen S.

PY - 2009/7/1

Y1 - 2009/7/1

N2 - This study was undertaken to determine the antibacterial activity of eight cationic antimicrobial peptides towards strains of genomovars I–V of the Burkholderia cepacia complex (Bcc) in time–kill assays. All but one of the peptides failed to show activity against the panel of test strains. The exception was magainin II, a 23 aa peptide isolated from the epidermis of the African clawed frog, Xenopus laevis, which exhibited significant bactericidal activity for Bcc genomovars most frequently associated with lung infection of patients with cystic fibrosis. In vitro studies indicated that magainin II protected a human bronchial epithelial cell line (BEAS-2B) from killing by Bcc and suggest that this peptide may have therapeutic potential against these organisms.

AB - This study was undertaken to determine the antibacterial activity of eight cationic antimicrobial peptides towards strains of genomovars I–V of the Burkholderia cepacia complex (Bcc) in time–kill assays. All but one of the peptides failed to show activity against the panel of test strains. The exception was magainin II, a 23 aa peptide isolated from the epidermis of the African clawed frog, Xenopus laevis, which exhibited significant bactericidal activity for Bcc genomovars most frequently associated with lung infection of patients with cystic fibrosis. In vitro studies indicated that magainin II protected a human bronchial epithelial cell line (BEAS-2B) from killing by Bcc and suggest that this peptide may have therapeutic potential against these organisms.

KW - cationic antimicrobial peptides

KW - Burkholderia cepacia

KW - cystic fibrosis

U2 - 10.1099/jmm.0.008128-0

DO - 10.1099/jmm.0.008128-0

M3 - Article

C2 - 19502364

AN - SCOPUS:67650169482

SN - 0022-2615

VL - 58

SP - 923

EP - 929

JO - Journal of Medical Microbiology

JF - Journal of Medical Microbiology

IS - 7

ER -

The cationic peptide magainin II is antimicrobial for Burkholderia cepacia-complex strains

Abstract

Keywords

Access to Document

Fingerprint

Cite this