The AT-Hook motif as a versatile minor groove anchor for promoting DNA binding of transcription factor fragments

Jéssica Rodríguez; Jesús Mosquera; Jose R. Couceiro; M. Eugenio Vázquez; José L. Mascareñas

doi:10.1039/c5sc01415h

The AT-Hook motif as a versatile minor groove anchor for promoting DNA binding of transcription factor fragments

Jéssica Rodríguez, Jesús Mosquera, Jose R. Couceiro, M. Eugenio Vázquez^*, José L. Mascareñas

^*Corresponding author for this work

Pure And Applied Chemistry

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

We report the development of chimeric DNA binding peptides comprising a DNA binding fragment of natural transcription factors (the basic region of a bZIP protein or a monomeric zinc finger module) and an AT-Hook peptide motif. The resulting peptide conjugates display high DNA affinity and excellent sequence selectivity. Furthermore, the AT-Hook motif also favors the cell internalization of the conjugates.

Original language	English
Pages (from-to)	4767-4771
Number of pages	5
Journal	Chemical Science
Volume	6
Issue number	8
DOIs	https://doi.org/10.1039/c5sc01415h
Publication status	Published - 1 Aug 2015

Keywords

DNA binding
chimeric DNA binding peptides
AT-Hook motif

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10.1039/c5sc01415h

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abstract = "We report the development of chimeric DNA binding peptides comprising a DNA binding fragment of natural transcription factors (the basic region of a bZIP protein or a monomeric zinc finger module) and an AT-Hook peptide motif. The resulting peptide conjugates display high DNA affinity and excellent sequence selectivity. Furthermore, the AT-Hook motif also favors the cell internalization of the conjugates.",

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AU - Rodríguez, Jéssica

AU - Mosquera, Jesús

AU - Couceiro, Jose R.

AU - Vázquez, M. Eugenio

AU - Mascareñas, José L.

PY - 2015/8/1

Y1 - 2015/8/1

N2 - We report the development of chimeric DNA binding peptides comprising a DNA binding fragment of natural transcription factors (the basic region of a bZIP protein or a monomeric zinc finger module) and an AT-Hook peptide motif. The resulting peptide conjugates display high DNA affinity and excellent sequence selectivity. Furthermore, the AT-Hook motif also favors the cell internalization of the conjugates.

AB - We report the development of chimeric DNA binding peptides comprising a DNA binding fragment of natural transcription factors (the basic region of a bZIP protein or a monomeric zinc finger module) and an AT-Hook peptide motif. The resulting peptide conjugates display high DNA affinity and excellent sequence selectivity. Furthermore, the AT-Hook motif also favors the cell internalization of the conjugates.

KW - DNA binding

KW - chimeric DNA binding peptides

KW - AT-Hook motif

UR - https://www.scopus.com/pages/publications/84937239277

U2 - 10.1039/c5sc01415h

DO - 10.1039/c5sc01415h

M3 - Article

AN - SCOPUS:84937239277

SN - 2041-6520

VL - 6

SP - 4767

EP - 4771

JO - Chemical Science

JF - Chemical Science

IS - 8

ER -

The AT-Hook motif as a versatile minor groove anchor for promoting DNA binding of transcription factor fragments

Abstract

Keywords

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