The antiprotozoal activity of Papua New Guinea propolis and its triterpenes

Samya S. Alenezi; Naif D. Alenzi; Godwin U. Ebiloma; Manal J. Natto; Marzuq A. Ungogo; John O. Igoli; Valerie A. Ferro; Alexander I. Gray; James Fearnley; Harry P. de Koning; David G. Watson

doi:10.3390/molecules27051622

The antiprotozoal activity of Papua New Guinea propolis and its triterpenes

Samya S. Alenezi, Naif D. Alenzi, Godwin U. Ebiloma, Manal J. Natto, Marzuq A. Ungogo, John O. Igoli, Valerie A. Ferro, Alexander I. Gray, James Fearnley, Harry P. de Koning, David G. Watson

Strathclyde Institute Of Pharmacy And Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

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Abstract

Profiling a propolis sample from Papua New Guinea (PNG) using high-resolution mass spectrometry indicated that it contained several triterpenoids. Further fractionation by column chromatography and medium-pressure liquid chromatography (MPLC) followed by nuclear magnetic resonance spectroscopy (NMR) identified 12 triterpenoids. Five of these were obtained pure and the others as mixtures of two or three compounds. The compounds identified were: mangiferonic acid, ambonic acid, isomangiferolic acid, ambolic acid, 27-hydroxyisomangiferolic acid, cycloartenol, cycloeucalenol, 24-methylenecycloartenol, 20-hydroxybetulin, betulin, betulinic acid and madecassic acid. The fractions from the propolis and the purified compounds were tested in vitro against Crithidia fasciculata, Trypanosoma congolense, drug-resistant Trypanosoma congolense, Trypanosoma b. brucei and multidrug-resistant Trypanosoma b. brucei (B48). They were also assayed for their toxicity against U947 cells. The compounds and fractions displayed moderate to high activity against parasitic protozoa but only low cytotoxicity against the mammalian cells. The most active isolated compound, 20-hydroxybetulin, was found to be trypanostatic when different concentrations were tested against T. b. brucei growth.

Original language	English
Article number	1622
Number of pages	15
Journal	Molecules
Volume	27
Issue number	5
Early online date	1 Mar 2022
DOIs	https://doi.org/10.3390/molecules27051622
Publication status	Published - 1 Mar 2022

Keywords

triterpenes
T.. brucei
T.congolenese
Papua New Guinea
propolis
U947 cells

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10.3390/molecules27051622Licence: CC BY 4.0

Alenezi-etal-Molecules-2022-The-antiprotozoal-activity-of-Papua-New-Guinea-propolis-and-its-triterpenesFinal published version, 459 KBLicence: CC BY 4.0

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title = "The antiprotozoal activity of Papua New Guinea propolis and its triterpenes",

abstract = "Profiling a propolis sample from Papua New Guinea (PNG) using high-resolution mass spectrometry indicated that it contained several triterpenoids. Further fractionation by column chromatography and medium-pressure liquid chromatography (MPLC) followed by nuclear magnetic resonance spectroscopy (NMR) identified 12 triterpenoids. Five of these were obtained pure and the others as mixtures of two or three compounds. The compounds identified were: mangiferonic acid, ambonic acid, isomangiferolic acid, ambolic acid, 27-hydroxyisomangiferolic acid, cycloartenol, cycloeucalenol, 24-methylenecycloartenol, 20-hydroxybetulin, betulin, betulinic acid and madecassic acid. The fractions from the propolis and the purified compounds were tested in vitro against Crithidia fasciculata, Trypanosoma congolense, drug-resistant Trypanosoma congolense, Trypanosoma b. brucei and multidrug-resistant Trypanosoma b. brucei (B48). They were also assayed for their toxicity against U947 cells. The compounds and fractions displayed moderate to high activity against parasitic protozoa but only low cytotoxicity against the mammalian cells. The most active isolated compound, 20-hydroxybetulin, was found to be trypanostatic when different concentrations were tested against T. b. brucei growth.",

keywords = "triterpenes, T.. brucei, T.congolenese, Papua New Guinea, propolis, U947 cells",

author = "Alenezi, {Samya S.} and Alenzi, {Naif D.} and Ebiloma, {Godwin U.} and Natto, {Manal J.} and Ungogo, {Marzuq A.} and Igoli, {John O.} and Ferro, {Valerie A.} and Gray, {Alexander I.} and James Fearnley and {de Koning}, {Harry P.} and Watson, {David G.}",

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AU - Alenezi, Samya S.

AU - Alenzi, Naif D.

AU - Ebiloma, Godwin U.

AU - Natto, Manal J.

AU - Ungogo, Marzuq A.

AU - Igoli, John O.

AU - Ferro, Valerie A.

AU - Gray, Alexander I.

AU - Fearnley, James

AU - de Koning, Harry P.

AU - Watson, David G.

PY - 2022/3/1

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N2 - Profiling a propolis sample from Papua New Guinea (PNG) using high-resolution mass spectrometry indicated that it contained several triterpenoids. Further fractionation by column chromatography and medium-pressure liquid chromatography (MPLC) followed by nuclear magnetic resonance spectroscopy (NMR) identified 12 triterpenoids. Five of these were obtained pure and the others as mixtures of two or three compounds. The compounds identified were: mangiferonic acid, ambonic acid, isomangiferolic acid, ambolic acid, 27-hydroxyisomangiferolic acid, cycloartenol, cycloeucalenol, 24-methylenecycloartenol, 20-hydroxybetulin, betulin, betulinic acid and madecassic acid. The fractions from the propolis and the purified compounds were tested in vitro against Crithidia fasciculata, Trypanosoma congolense, drug-resistant Trypanosoma congolense, Trypanosoma b. brucei and multidrug-resistant Trypanosoma b. brucei (B48). They were also assayed for their toxicity against U947 cells. The compounds and fractions displayed moderate to high activity against parasitic protozoa but only low cytotoxicity against the mammalian cells. The most active isolated compound, 20-hydroxybetulin, was found to be trypanostatic when different concentrations were tested against T. b. brucei growth.

AB - Profiling a propolis sample from Papua New Guinea (PNG) using high-resolution mass spectrometry indicated that it contained several triterpenoids. Further fractionation by column chromatography and medium-pressure liquid chromatography (MPLC) followed by nuclear magnetic resonance spectroscopy (NMR) identified 12 triterpenoids. Five of these were obtained pure and the others as mixtures of two or three compounds. The compounds identified were: mangiferonic acid, ambonic acid, isomangiferolic acid, ambolic acid, 27-hydroxyisomangiferolic acid, cycloartenol, cycloeucalenol, 24-methylenecycloartenol, 20-hydroxybetulin, betulin, betulinic acid and madecassic acid. The fractions from the propolis and the purified compounds were tested in vitro against Crithidia fasciculata, Trypanosoma congolense, drug-resistant Trypanosoma congolense, Trypanosoma b. brucei and multidrug-resistant Trypanosoma b. brucei (B48). They were also assayed for their toxicity against U947 cells. The compounds and fractions displayed moderate to high activity against parasitic protozoa but only low cytotoxicity against the mammalian cells. The most active isolated compound, 20-hydroxybetulin, was found to be trypanostatic when different concentrations were tested against T. b. brucei growth.

KW - triterpenes

KW - T.. brucei

KW - T.congolenese

KW - Papua New Guinea

KW - propolis

KW - U947 cells

U2 - 10.3390/molecules27051622

DO - 10.3390/molecules27051622

M3 - Article

SN - 1420-3049

VL - 27

JO - Molecules

JF - Molecules

IS - 5

M1 - 1622

ER -

The antiprotozoal activity of Papua New Guinea propolis and its triterpenes

Abstract

Keywords

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