Abstract
The actions of the anticholinesterase drugs, physostigmine, neostigmine and diisopropylfluorophosphate (DFP) on chick embryonic skeletal muscle in culture were studied. None of the anticholinesterases potentiated depolarization responses to acetylcholine. In high concentrations neostigmine and physostigmine produced depolarization. The neostigmine‐induced, but not the physostigmine‐induced, depolarization was antagonized by tubocurarine. DFP caused an increase in the rate of repolarization during the presence of a cholinomimetic. It is concluded that the cholinesterase present in cultured muscle fibres does not have a physiological role in hydrolysing acetylcholine and that physostigmine and DFP have an action at the ionic channels that are linked to the cholinoreceptor.
| Original language | English |
|---|---|
| Pages (from-to) | 865-870 |
| Number of pages | 6 |
| Journal | Journal of Pharmacy and Pharmacology |
| Volume | 26 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - 1 Jan 1974 |
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Keywords
- acetylcholine
- carbachol
- cholinergic receptor
- cholinesterase inhibitor
- dyflos
- neostigmine
- neostigmine methyl sulfate
- physostigmine
- tubocurarine chloride
- in vitro study
- muscle cell
Cite this
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The actions of some anticholinesterase drugs on skeletal muscle in culture. / HARVEY, A. L.; DRYDEN, W. F.
In: Journal of Pharmacy and Pharmacology, Vol. 26, No. 11, 01.01.1974, p. 865-870.Research output: Contribution to journal › Article
TY - JOUR
T1 - The actions of some anticholinesterase drugs on skeletal muscle in culture
AU - HARVEY, A. L.
AU - DRYDEN, W. F.
PY - 1974/1/1
Y1 - 1974/1/1
N2 - The actions of the anticholinesterase drugs, physostigmine, neostigmine and diisopropylfluorophosphate (DFP) on chick embryonic skeletal muscle in culture were studied. None of the anticholinesterases potentiated depolarization responses to acetylcholine. In high concentrations neostigmine and physostigmine produced depolarization. The neostigmine‐induced, but not the physostigmine‐induced, depolarization was antagonized by tubocurarine. DFP caused an increase in the rate of repolarization during the presence of a cholinomimetic. It is concluded that the cholinesterase present in cultured muscle fibres does not have a physiological role in hydrolysing acetylcholine and that physostigmine and DFP have an action at the ionic channels that are linked to the cholinoreceptor.
AB - The actions of the anticholinesterase drugs, physostigmine, neostigmine and diisopropylfluorophosphate (DFP) on chick embryonic skeletal muscle in culture were studied. None of the anticholinesterases potentiated depolarization responses to acetylcholine. In high concentrations neostigmine and physostigmine produced depolarization. The neostigmine‐induced, but not the physostigmine‐induced, depolarization was antagonized by tubocurarine. DFP caused an increase in the rate of repolarization during the presence of a cholinomimetic. It is concluded that the cholinesterase present in cultured muscle fibres does not have a physiological role in hydrolysing acetylcholine and that physostigmine and DFP have an action at the ionic channels that are linked to the cholinoreceptor.
KW - acetylcholine
KW - carbachol
KW - cholinergic receptor
KW - cholinesterase inhibitor
KW - dyflos
KW - neostigmine
KW - neostigmine methyl sulfate
KW - physostigmine
KW - tubocurarine chloride
KW - in vitro study
KW - muscle cell
UR - http://www.scopus.com/inward/record.url?scp=0016277084&partnerID=8YFLogxK
U2 - 10.1111/j.2042-7158.1974.tb09194.x
DO - 10.1111/j.2042-7158.1974.tb09194.x
M3 - Article
C2 - 4156556
AN - SCOPUS:0016277084
VL - 26
SP - 865
EP - 870
JO - Journal of Pharmacy and Pharmacology
JF - Journal of Pharmacy and Pharmacology
SN - 0022-3573
IS - 11
ER -