The actions of some anticholinesterase drugs on skeletal muscle in culture

A. L. HARVEY, W. F. DRYDEN

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8 Citations (Scopus)

Abstract

The actions of the anticholinesterase drugs, physostigmine, neostigmine and diisopropylfluorophosphate (DFP) on chick embryonic skeletal muscle in culture were studied. None of the anticholinesterases potentiated depolarization responses to acetylcholine. In high concentrations neostigmine and physostigmine produced depolarization. The neostigmine‐induced, but not the physostigmine‐induced, depolarization was antagonized by tubocurarine. DFP caused an increase in the rate of repolarization during the presence of a cholinomimetic. It is concluded that the cholinesterase present in cultured muscle fibres does not have a physiological role in hydrolysing acetylcholine and that physostigmine and DFP have an action at the ionic channels that are linked to the cholinoreceptor.

Original languageEnglish
Pages (from-to)865-870
Number of pages6
JournalJournal of Pharmacy and Pharmacology
Volume26
Issue number11
DOIs
Publication statusPublished - 1 Jan 1974

Keywords

  • acetylcholine
  • carbachol
  • cholinergic receptor
  • cholinesterase inhibitor
  • dyflos
  • neostigmine
  • neostigmine methyl sulfate
  • physostigmine
  • tubocurarine chloride
  • in vitro study
  • muscle cell

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