The ββα fold of zinc finger proteins as a “natural” protecting group. Chemoselective synthesis of a DNA-binding zinc finger derivative

Jéssica Rodríguez, Jesús Mosquera, Olalla Vázquez, M. Eugenio Vázquez, José L. Mascareñas

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We report the selective modification of cysteine residues engineered in peptides that have two additional cysteine residues as part of a Cys2His2 zinc finger motif. The chemoselective modification is achieved, thanks to the protecting effect exerted by the zinc cation upon coordination with the native cysteines and histidines of the zinc-finger fold. The strategy allows a straightforward synthesis of DNA binding zinc finger constructs.

Original languageEnglish
Pages (from-to)2258-2260
Number of pages3
JournalChemical Communications
Volume50
Issue number18
DOIs
Publication statusPublished - 4 Feb 2014

Keywords

  • cysteine residues
  • zinc finger proteins

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