Terahertz underdamped vibrational motion governs protein-ligand binding in solution

David A. Turton, Hans Martin Senn, Thomas Harwood, Adrian J. Lapthorn, Elizabeth M. Ellis, Klaas Wynne

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

Low-frequency collective vibrational modes in proteins have been proposed as being responsible for efficiently directing biochemical reactions and biological energy transport. However, evidence of the existence of delocalized vibrational modes is scarce and proof of their involvement in biological function absent. Here we apply extremely sensitive femtosecond optical Kerr-effect spectroscopy to study the depolarized Raman spectra of lysozyme and its complex with the inhibitor triacetylchitotriose in solution. Underdamped delocalized vibrational modes in the terahertz frequency domain are identified and shown to blue-shift and strengthen upon inhibitor binding. This demonstrates that the ligand-binding coordinate in proteins is underdamped and not simply solvent-controlled as previously assumed. The presence of such underdamped delocalized modes in proteins may have significant implications for the understanding of the efficiency of ligand binding and protein-molecule interactions, and has wider implications for biochemical reactivity and biological function.

LanguageEnglish
Article number3999
Number of pages5
JournalNature Communications
Volume5
DOIs
Publication statusPublished - 3 Jun 2014

Fingerprint

Protein Binding
Ligands
proteins
vibration mode
ligands
Biological Transport
inhibitors
Optical Kerr effect
Proteins
Vibrational spectra
Muramidase
Raman scattering
Spectrum Analysis
lysozyme
Carrier Proteins
Kerr effects
blue shift
Spectroscopy
Molecules
reactivity

Keywords

  • low-frequency collective vibrational modes
  • biochemical reactions
  • biological energy transport
  • terahertz frequency
  • ligand binding
  • protein–molecule interactions

Cite this

Turton, D. A., Senn, H. M., Harwood, T., Lapthorn, A. J., Ellis, E. M., & Wynne, K. (2014). Terahertz underdamped vibrational motion governs protein-ligand binding in solution. Nature Communications, 5, [3999]. https://doi.org/10.1038/ncomms4999
Turton, David A. ; Senn, Hans Martin ; Harwood, Thomas ; Lapthorn, Adrian J. ; Ellis, Elizabeth M. ; Wynne, Klaas. / Terahertz underdamped vibrational motion governs protein-ligand binding in solution. In: Nature Communications. 2014 ; Vol. 5.
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Turton, DA, Senn, HM, Harwood, T, Lapthorn, AJ, Ellis, EM & Wynne, K 2014, 'Terahertz underdamped vibrational motion governs protein-ligand binding in solution' Nature Communications, vol. 5, 3999. https://doi.org/10.1038/ncomms4999

Terahertz underdamped vibrational motion governs protein-ligand binding in solution. / Turton, David A.; Senn, Hans Martin; Harwood, Thomas; Lapthorn, Adrian J.; Ellis, Elizabeth M.; Wynne, Klaas.

In: Nature Communications, Vol. 5, 3999, 03.06.2014.

Research output: Contribution to journalArticle

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