Abstract
Widefield standing wave microscopy has been shown to provide axial resolutions below 100 nm that can be acquired at up to 100 frames per second with the only change to the imaging setup being there placement of a standard microscope slide with a first surface reflector[1,2]. However, because this technique makes use of the interaction between a fluorescent specimen and the antinodal planes of an optical standing wave to achieve axial super-resolution the nodal plane contributions result in ~50% of the specimen not being imaged. We present a method called TartanSW which makes use of standing waves of different wavelengths to shift the antinodal plane axial locations and hence reduce the amount of missing axial information in the mage.
Original language | English |
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Number of pages | 1 |
Publication status | Published - 5 Jun 2019 |
Event | Photonex - Technology & Innovation Centre, Glasgow, United Kingdom Duration: 5 Jun 2019 → 5 Jun 2019 |
Conference
Conference | Photonex |
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Country/Territory | United Kingdom |
City | Glasgow |
Period | 5/06/19 → 5/06/19 |
Keywords
- microscopy
- microscopy images
- standing waves