TartanSW: filling the information gap in standing wave microscopy

Research output: Contribution to conferencePoster

Abstract

Widefield standing wave microscopy has been shown to provide axial resolutions below 100 nm that can be acquired at up to 100 frames per second with the only change to the imaging setup being there placement of a standard microscope slide with a first surface reflector[1,2]. However, because this technique makes use of the interaction between a fluorescent specimen and the antinodal planes of an optical standing wave to achieve axial super-resolution the nodal plane contributions result in ~50% of the specimen not being imaged. We present a method called TartanSW which makes use of standing waves of different wavelengths to shift the antinodal plane axial locations and hence reduce the amount of missing axial information in the mage.
Original languageEnglish
Number of pages1
Publication statusPublished - 5 Jun 2019
EventPhotonex - Technology & Innovation Centre, Glasgow, United Kingdom
Duration: 5 Jun 20195 Jun 2019

Conference

ConferencePhotonex
CountryUnited Kingdom
CityGlasgow
Period5/06/195/06/19

Fingerprint

standing waves
microscopy
chutes
reflectors
microscopes
shift
wavelengths
interactions

Keywords

  • microscopy
  • microscopy images
  • standing waves

Cite this

Tinning, P. W., Schniete, J. K., Scrimgeour, R., & McConnell, G. (2019). TartanSW: filling the information gap in standing wave microscopy. Poster session presented at Photonex, Glasgow, United Kingdom.
Tinning, P. W. ; Schniete, J. K. ; Scrimgeour, R. ; McConnell, G. / TartanSW : filling the information gap in standing wave microscopy. Poster session presented at Photonex, Glasgow, United Kingdom.1 p.
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author = "Tinning, {P. W.} and Schniete, {J. K.} and R. Scrimgeour and G. McConnell",
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Tinning, PW, Schniete, JK, Scrimgeour, R & McConnell, G 2019, 'TartanSW: filling the information gap in standing wave microscopy', Photonex, Glasgow, United Kingdom, 5/06/19 - 5/06/19.

TartanSW : filling the information gap in standing wave microscopy. / Tinning, P. W.; Schniete, J. K.; Scrimgeour, R.; McConnell, G.

2019. Poster session presented at Photonex, Glasgow, United Kingdom.

Research output: Contribution to conferencePoster

TY - CONF

T1 - TartanSW

T2 - filling the information gap in standing wave microscopy

AU - Tinning, P. W.

AU - Schniete, J. K.

AU - Scrimgeour, R.

AU - McConnell, G.

PY - 2019/6/5

Y1 - 2019/6/5

N2 - Widefield standing wave microscopy has been shown to provide axial resolutions below 100 nm that can be acquired at up to 100 frames per second with the only change to the imaging setup being there placement of a standard microscope slide with a first surface reflector[1,2]. However, because this technique makes use of the interaction between a fluorescent specimen and the antinodal planes of an optical standing wave to achieve axial super-resolution the nodal plane contributions result in ~50% of the specimen not being imaged. We present a method called TartanSW which makes use of standing waves of different wavelengths to shift the antinodal plane axial locations and hence reduce the amount of missing axial information in the mage.

AB - Widefield standing wave microscopy has been shown to provide axial resolutions below 100 nm that can be acquired at up to 100 frames per second with the only change to the imaging setup being there placement of a standard microscope slide with a first surface reflector[1,2]. However, because this technique makes use of the interaction between a fluorescent specimen and the antinodal planes of an optical standing wave to achieve axial super-resolution the nodal plane contributions result in ~50% of the specimen not being imaged. We present a method called TartanSW which makes use of standing waves of different wavelengths to shift the antinodal plane axial locations and hence reduce the amount of missing axial information in the mage.

KW - microscopy

KW - microscopy images

KW - standing waves

UR - https://www.photonexroadshow.com/

M3 - Poster

ER -

Tinning PW, Schniete JK, Scrimgeour R, McConnell G. TartanSW: filling the information gap in standing wave microscopy. 2019. Poster session presented at Photonex, Glasgow, United Kingdom.