Synthetic biological approaches for RNA labelling and imaging: design principles and future opportunities

Research output: Contribution to journalReview article

7 Citations (Scopus)

Abstract

RNA is the most mercurial of all biomacromolecules. In contrast to DNA, where the predominant role is the storage of genetic information, the biological role of RNA varies; ranging from a template-based intermediary in gene expression to playing a direct role in catalysis. Their high turnover and metabolic lability makes the detection of specific sequences particularly challenging. This review describes the latest synthetic biological developments that enable the direct imaging of RNA both in vitro and in their native cellular environment.

LanguageEnglish
Pages153-158
Number of pages6
JournalCurrent Opinion in Biotechnology
Volume48
Early online date8 May 2017
DOIs
Publication statusPublished - 1 Dec 2017

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RNA
Labeling
Imaging techniques
Information Storage and Retrieval
Catalysis
Gene expression
DNA
Gene Expression

Keywords

  • RNA
  • biomacromolecules
  • RNA imaging

Cite this

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abstract = "RNA is the most mercurial of all biomacromolecules. In contrast to DNA, where the predominant role is the storage of genetic information, the biological role of RNA varies; ranging from a template-based intermediary in gene expression to playing a direct role in catalysis. Their high turnover and metabolic lability makes the detection of specific sequences particularly challenging. This review describes the latest synthetic biological developments that enable the direct imaging of RNA both in vitro and in their native cellular environment.",
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AU - Pauff, Steven

AU - Withers, Jamie M

AU - McKean, Iain JW

AU - Mackay, Simon P

AU - Burley, Glenn A

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KW - RNA imaging

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