Synthetic biological approaches for RNA labelling and imaging: design principles and future opportunities

Steven Pauff; Jamie M Withers; Iain JW McKean; Simon P Mackay; Glenn A Burley

doi:10.1016/j.copbio.2017.04.003

Synthetic biological approaches for RNA labelling and imaging: design principles and future opportunities

Steven Pauff, Jamie M Withers, Iain JW McKean, Simon P Mackay, Glenn A Burley

Research output: Contribution to journal › Review article › peer-review

9 Citations (Scopus)

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Abstract

RNA is the most mercurial of all biomacromolecules. In contrast to DNA, where the predominant role is the storage of genetic information, the biological role of RNA varies; ranging from a template-based intermediary in gene expression to playing a direct role in catalysis. Their high turnover and metabolic lability makes the detection of specific sequences particularly challenging. This review describes the latest synthetic biological developments that enable the direct imaging of RNA both in vitro and in their native cellular environment.

Original language	English
Pages (from-to)	153-158
Number of pages	6
Journal	Current Opinion in Biotechnology
Volume	48
Early online date	8 May 2017
DOIs	https://doi.org/10.1016/j.copbio.2017.04.003
Publication status	Published - 1 Dec 2017

Keywords

RNA
biomacromolecules
RNA imaging

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10.1016/j.copbio.2017.04.003

Pauff-etal-COB-2017-Synthetic-biological-approaches-for-RNA-labelling-and-imaging-design-principlesAccepted author manuscript, 882 KBLicence: OGL_3

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title = "Synthetic biological approaches for RNA labelling and imaging: design principles and future opportunities",

abstract = "RNA is the most mercurial of all biomacromolecules. In contrast to DNA, where the predominant role is the storage of genetic information, the biological role of RNA varies; ranging from a template-based intermediary in gene expression to playing a direct role in catalysis. Their high turnover and metabolic lability makes the detection of specific sequences particularly challenging. This review describes the latest synthetic biological developments that enable the direct imaging of RNA both in vitro and in their native cellular environment.",

keywords = "RNA, biomacromolecules, RNA imaging",

author = "Steven Pauff and Withers, {Jamie M} and McKean, {Iain JW} and Mackay, {Simon P} and Burley, {Glenn A}",

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doi = "10.1016/j.copbio.2017.04.003",

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TY - JOUR

T1 - Synthetic biological approaches for RNA labelling and imaging

T2 - design principles and future opportunities

AU - Pauff, Steven

AU - Withers, Jamie M

AU - McKean, Iain JW

AU - Mackay, Simon P

AU - Burley, Glenn A

N1 - Published under Crown Copyright (Open Government Licence 3.0).

PY - 2017/12/1

Y1 - 2017/12/1

N2 - RNA is the most mercurial of all biomacromolecules. In contrast to DNA, where the predominant role is the storage of genetic information, the biological role of RNA varies; ranging from a template-based intermediary in gene expression to playing a direct role in catalysis. Their high turnover and metabolic lability makes the detection of specific sequences particularly challenging. This review describes the latest synthetic biological developments that enable the direct imaging of RNA both in vitro and in their native cellular environment.

AB - RNA is the most mercurial of all biomacromolecules. In contrast to DNA, where the predominant role is the storage of genetic information, the biological role of RNA varies; ranging from a template-based intermediary in gene expression to playing a direct role in catalysis. Their high turnover and metabolic lability makes the detection of specific sequences particularly challenging. This review describes the latest synthetic biological developments that enable the direct imaging of RNA both in vitro and in their native cellular environment.

KW - RNA

KW - biomacromolecules

KW - RNA imaging

UR - http://www.sciencedirect.com/science/journal/09581669?sdc=1

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U2 - 10.1016/j.copbio.2017.04.003

DO - 10.1016/j.copbio.2017.04.003

M3 - Review article

AN - SCOPUS:85018381385

SN - 0958-1669

VL - 48

SP - 153

EP - 158

JO - Current Opinion in Biotechnology

JF - Current Opinion in Biotechnology

ER -

Synthetic biological approaches for RNA labelling and imaging: design principles and future opportunities

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Keywords

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Establishing an integrated biocatalytic Friedel-Crafts platform for the alkylation of aromatic small molecules | McKean, Iain

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Synthetic biological approaches for RNA labelling and imaging: design principles and future opportunities

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Establishing an integrated biocatalytic Friedel-Crafts platform for the alkylation of aromatic small molecules | McKean, Iain

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