Synthesis, structural elucidation, DNA-PK inhibition, homology modelling and anti-platelet activity of morpholino-substituted-1,3-naphth-oxazines

S. Ihmaid, J. Al-Rawi, C. Bradley, M. J. Angove, M. N. Robertson, R. L. Clark

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)


A number of new angular 2-morpholino-(substituted)-naphth-1,3-oxazines (compound 10b), linear 2-morpholino-(substituted)-naphth-1,3-oxazines (compounds 13b-c), linear 6, 7 and 9-0-substituted-2-morpholino-(substituted)-naphth-1,3-oxazines (compounds 17-22, 24, and 25) and angular compounds 14-16 and 23 were synthesised. The O-substituent was pyridin-2yl-methyl (15, 18, and 21) pyridin-3yl-methyl (16, 19, and 22) and 4-methylpipreazin-1-yl-ethoxy (23-25). Twelve compounds were tested for their inhibitory effect on collagen induced platelet aggregation and it was found that the most active compounds were compounds 19 and 22 with IC(50) = 55 +/- 4 and 85 +/- 4 mu M, respectively. Furthermore, the compounds were also assayed for their ability to inhibit DNA-dependent protein kinase (DNA-PK) activity. The most active compounds were 18 IC(50) = 0.091 mu M, 24 IC(50) = 0.191 mu M, and 22 IC(50) = 0.331 mu M.

Homology modelling was used to build a 3D model of DNA-PK based on the X-ray structure of phosphatidylinositol 3-kinases (PI3Ks). Docking of synthesised compounds within the binding pocket and structure-activity relationships (SAR) analyses of the poses were performed and results agreed well with observed activity.
Original languageEnglish
Pages (from-to)3983-3994
Number of pages12
JournalBioorganic and Medicinal Chemistry
Issue number13
Publication statusPublished - 1 Jul 2011


  • dependent protein-kinase
  • anti-platelet activity
  • potent
  • identification
  • DNA-PK IC50
  • autophosphorylation
  • docking
  • morpholino-1,3-naphth-oxazine
  • catalytic subunit
  • LY294002
  • structural elucidation
  • homology modelling
  • morpholino-substituted-1,3-naphth-oxazines


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