Synthesis of a benzotriazole phosphoramidite for attachment of oligonucleotides to metal surfaces

R. Brown; W.E. Smith; D. Graham

doi:10.1016/S0040-4039(01)00108-3

Synthesis of a benzotriazole phosphoramidite for attachment of oligonucleotides to metal surfaces

R. Brown, W.E. Smith, D. Graham

Pure And Applied Chemistry

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

A method for the addition of a benzotriazole moiety to the 5'-terminus of an oligonucleotide via phosphoramidite chemistry has been developed. Use of a monomethoxytrityl protecting group on the benzotriazole allowed fast on-column detritylation purification by reverse-phase HPLC. Surface enhanced Raman scattering (SERS) of the modified oligonucleotides was obtained from silver colloid.

Original language	English
Pages (from-to)	2197-2200
Number of pages	3
Journal	Tetrahedron Letters
Volume	42
Issue number	11
DOIs	https://doi.org/10.1016/S0040-4039(01)00108-3
Publication status	Published - 11 Mar 2001

Keywords

HPLC
nucleic acids
phosphoramidites
solid-phase synthesis
SERS

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10.1016/S0040-4039(01)00108-3

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abstract = "A method for the addition of a benzotriazole moiety to the 5'-terminus of an oligonucleotide via phosphoramidite chemistry has been developed. Use of a monomethoxytrityl protecting group on the benzotriazole allowed fast on-column detritylation purification by reverse-phase HPLC. Surface enhanced Raman scattering (SERS) of the modified oligonucleotides was obtained from silver colloid.",

keywords = "HPLC, nucleic acids, phosphoramidites, solid-phase synthesis, SERS",

author = "R. Brown and W.E. Smith and D. Graham",

year = "2001",

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T1 - Synthesis of a benzotriazole phosphoramidite for attachment of oligonucleotides to metal surfaces

AU - Brown, R.

AU - Smith, W.E.

AU - Graham, D.

PY - 2001/3/11

Y1 - 2001/3/11

N2 - A method for the addition of a benzotriazole moiety to the 5'-terminus of an oligonucleotide via phosphoramidite chemistry has been developed. Use of a monomethoxytrityl protecting group on the benzotriazole allowed fast on-column detritylation purification by reverse-phase HPLC. Surface enhanced Raman scattering (SERS) of the modified oligonucleotides was obtained from silver colloid.

AB - A method for the addition of a benzotriazole moiety to the 5'-terminus of an oligonucleotide via phosphoramidite chemistry has been developed. Use of a monomethoxytrityl protecting group on the benzotriazole allowed fast on-column detritylation purification by reverse-phase HPLC. Surface enhanced Raman scattering (SERS) of the modified oligonucleotides was obtained from silver colloid.

KW - HPLC

KW - nucleic acids

KW - phosphoramidites

KW - solid-phase synthesis

KW - SERS

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U2 - 10.1016/S0040-4039(01)00108-3

DO - 10.1016/S0040-4039(01)00108-3

M3 - Article

SN - 0040-4039

VL - 42

SP - 2197

EP - 2200

JO - Tetrahedron Letters

JF - Tetrahedron Letters

IS - 11

ER -

Synthesis of a benzotriazole phosphoramidite for attachment of oligonucleotides to metal surfaces

Abstract

Keywords

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