Synthesis of a benzotriazole phosphoramidite for attachment of oligonucleotides to metal surfaces

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A method for the addition of a benzotriazole moiety to the 5'-terminus of an oligonucleotide via phosphoramidite chemistry has been developed. Use of a monomethoxytrityl protecting group on the benzotriazole allowed fast on-column detritylation purification by reverse-phase HPLC. Surface enhanced Raman scattering (SERS) of the modified oligonucleotides was obtained from silver colloid.
LanguageEnglish
Pages2197-2200
Number of pages3
JournalTetrahedron Letters
Volume42
Issue number11
DOIs
Publication statusPublished - 11 Mar 2001

Fingerprint

Oligonucleotides
Metals
Raman Spectrum Analysis
Distillation columns
Purification
Raman scattering
High Pressure Liquid Chromatography
benzotriazole
phosphoramidite
colloidal silver

Keywords

  • HPLC
  • nucleic acids
  • phosphoramidites
  • solid-phase synthesis
  • SERS

Cite this

@article{fabb83ffab4649788375e71ae6c2e2a4,
title = "Synthesis of a benzotriazole phosphoramidite for attachment of oligonucleotides to metal surfaces",
abstract = "A method for the addition of a benzotriazole moiety to the 5'-terminus of an oligonucleotide via phosphoramidite chemistry has been developed. Use of a monomethoxytrityl protecting group on the benzotriazole allowed fast on-column detritylation purification by reverse-phase HPLC. Surface enhanced Raman scattering (SERS) of the modified oligonucleotides was obtained from silver colloid.",
keywords = "HPLC, nucleic acids, phosphoramidites, solid-phase synthesis, SERS",
author = "R. Brown and W.E. Smith and D. Graham",
year = "2001",
month = "3",
day = "11",
doi = "10.1016/S0040-4039(01)00108-3",
language = "English",
volume = "42",
pages = "2197--2200",
journal = "Tetrahedron Letters",
issn = "0040-4039",
number = "11",

}

Synthesis of a benzotriazole phosphoramidite for attachment of oligonucleotides to metal surfaces. / Brown, R.; Smith, W.E.; Graham, D.

In: Tetrahedron Letters, Vol. 42, No. 11, 11.03.2001, p. 2197-2200.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Synthesis of a benzotriazole phosphoramidite for attachment of oligonucleotides to metal surfaces

AU - Brown, R.

AU - Smith, W.E.

AU - Graham, D.

PY - 2001/3/11

Y1 - 2001/3/11

N2 - A method for the addition of a benzotriazole moiety to the 5'-terminus of an oligonucleotide via phosphoramidite chemistry has been developed. Use of a monomethoxytrityl protecting group on the benzotriazole allowed fast on-column detritylation purification by reverse-phase HPLC. Surface enhanced Raman scattering (SERS) of the modified oligonucleotides was obtained from silver colloid.

AB - A method for the addition of a benzotriazole moiety to the 5'-terminus of an oligonucleotide via phosphoramidite chemistry has been developed. Use of a monomethoxytrityl protecting group on the benzotriazole allowed fast on-column detritylation purification by reverse-phase HPLC. Surface enhanced Raman scattering (SERS) of the modified oligonucleotides was obtained from silver colloid.

KW - HPLC

KW - nucleic acids

KW - phosphoramidites

KW - solid-phase synthesis

KW - SERS

UR - http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6THS-42FS7G3-1F-9&_cdi=5290&_user=875629&_orig=search&_coverDate=03%2F11%2F2001&_qd=1&_sk=999579988&view=c&wchp=dGLbVtz-zSkzk&md5=08b492b6bd11ef35faa7c05fa4a60485&ie=/sdarticle.pdf

UR - http://dx.doi.org/10.1016/S0040-4039(01)00108-3

U2 - 10.1016/S0040-4039(01)00108-3

DO - 10.1016/S0040-4039(01)00108-3

M3 - Article

VL - 42

SP - 2197

EP - 2200

JO - Tetrahedron Letters

T2 - Tetrahedron Letters

JF - Tetrahedron Letters

SN - 0040-4039

IS - 11

ER -