Synthesis of a benzotriazole phosphoramidite for attachment of oligonucleotides to metal surfaces

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Abstract

A method for the addition of a benzotriazole moiety to the 5'-terminus of an oligonucleotide via phosphoramidite chemistry has been developed. Use of a monomethoxytrityl protecting group on the benzotriazole allowed fast on-column detritylation purification by reverse-phase HPLC. Surface enhanced Raman scattering (SERS) of the modified oligonucleotides was obtained from silver colloid.
Original languageEnglish
Pages (from-to)2197-2200
Number of pages3
JournalTetrahedron Letters
Volume42
Issue number11
DOIs
Publication statusPublished - 11 Mar 2001

Keywords

  • HPLC
  • nucleic acids
  • phosphoramidites
  • solid-phase synthesis
  • SERS

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