A number of phosphoramidite monomers have been prepared and used in the synthesis of antisense phosphorothioate oligonucleotides bearing 5'-polyalkyl and cholesterol moieties. Similar groups have also been attached to the 3'-end of oligonucleotides by means of functionalised CPG. Melting temperatures of duplexes formed between phosphorothioate oligonucleotides with lipophilic end-groups and complementary DNA strands were found to be identical to those formed by the equivalent unmodified phosphorothioates.
|Number of pages||6|
|Journal||Nucleic Acids Research|
|Publication status||Published - 11 Jul 1992|
- human immunodeficiency virus
- phosphorothioate oligodeoxynucleotide