Synthesis and physical properties of anti-HIV antisense oligonucleotides bearing terminal lipophilic groups

C. Mackellar, D. Graham, D.W. Will, S. Burgess, T. Brown

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

A number of phosphoramidite monomers have been prepared and used in the synthesis of antisense phosphorothioate oligonucleotides bearing 5'-polyalkyl and cholesterol moieties. Similar groups have also been attached to the 3'-end of oligonucleotides by means of functionalised CPG. Melting temperatures of duplexes formed between phosphorothioate oligonucleotides with lipophilic end-groups and complementary DNA strands were found to be identical to those formed by the equivalent unmodified phosphorothioates.
LanguageEnglish
Pages3411-3417
Number of pages6
JournalNucleic Acids Research
Volume20
Issue number13
DOIs
Publication statusPublished - 11 Jul 1992

Fingerprint

Phosphorothioate Oligonucleotides
Antisense Oligonucleotides
HIV
Oligonucleotides
Freezing
Complementary DNA
Cholesterol
Temperature

Keywords

  • human immunodeficiency virus
  • phosphorothioate oligodeoxynucleotide
  • cells
  • derivatives
  • cholesterol
  • inhibitors
  • infection
  • invitro

Cite this

Mackellar, C. ; Graham, D. ; Will, D.W. ; Burgess, S. ; Brown, T. / Synthesis and physical properties of anti-HIV antisense oligonucleotides bearing terminal lipophilic groups. In: Nucleic Acids Research. 1992 ; Vol. 20, No. 13. pp. 3411-3417.
@article{160a1369a6c64687914f171b91f18f5e,
title = "Synthesis and physical properties of anti-HIV antisense oligonucleotides bearing terminal lipophilic groups",
abstract = "A number of phosphoramidite monomers have been prepared and used in the synthesis of antisense phosphorothioate oligonucleotides bearing 5'-polyalkyl and cholesterol moieties. Similar groups have also been attached to the 3'-end of oligonucleotides by means of functionalised CPG. Melting temperatures of duplexes formed between phosphorothioate oligonucleotides with lipophilic end-groups and complementary DNA strands were found to be identical to those formed by the equivalent unmodified phosphorothioates.",
keywords = "human immunodeficiency virus, phosphorothioate oligodeoxynucleotide, cells, derivatives, cholesterol, inhibitors, infection, invitro",
author = "C. Mackellar and D. Graham and D.W. Will and S. Burgess and T. Brown",
year = "1992",
month = "7",
day = "11",
doi = "10.1093/nar/20.13.3411",
language = "English",
volume = "20",
pages = "3411--3417",
journal = "Nucleic Acids Research",
issn = "0305-1048",
number = "13",

}

Synthesis and physical properties of anti-HIV antisense oligonucleotides bearing terminal lipophilic groups. / Mackellar, C.; Graham, D.; Will, D.W.; Burgess, S.; Brown, T.

In: Nucleic Acids Research, Vol. 20, No. 13, 11.07.1992, p. 3411-3417.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Synthesis and physical properties of anti-HIV antisense oligonucleotides bearing terminal lipophilic groups

AU - Mackellar, C.

AU - Graham, D.

AU - Will, D.W.

AU - Burgess, S.

AU - Brown, T.

PY - 1992/7/11

Y1 - 1992/7/11

N2 - A number of phosphoramidite monomers have been prepared and used in the synthesis of antisense phosphorothioate oligonucleotides bearing 5'-polyalkyl and cholesterol moieties. Similar groups have also been attached to the 3'-end of oligonucleotides by means of functionalised CPG. Melting temperatures of duplexes formed between phosphorothioate oligonucleotides with lipophilic end-groups and complementary DNA strands were found to be identical to those formed by the equivalent unmodified phosphorothioates.

AB - A number of phosphoramidite monomers have been prepared and used in the synthesis of antisense phosphorothioate oligonucleotides bearing 5'-polyalkyl and cholesterol moieties. Similar groups have also been attached to the 3'-end of oligonucleotides by means of functionalised CPG. Melting temperatures of duplexes formed between phosphorothioate oligonucleotides with lipophilic end-groups and complementary DNA strands were found to be identical to those formed by the equivalent unmodified phosphorothioates.

KW - human immunodeficiency virus

KW - phosphorothioate oligodeoxynucleotide

KW - cells

KW - derivatives

KW - cholesterol

KW - inhibitors

KW - infection

KW - invitro

UR - http://dx.doi.org/10.1093/nar/20.13.3411

U2 - 10.1093/nar/20.13.3411

DO - 10.1093/nar/20.13.3411

M3 - Article

VL - 20

SP - 3411

EP - 3417

JO - Nucleic Acids Research

T2 - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 13

ER -