Synthesis and physical properties of anti-HIV antisense oligonucleotides bearing terminal lipophilic groups

C. Mackellar; D. Graham; D.W. Will; S. Burgess; T. Brown

doi:10.1093/nar/20.13.3411

Synthesis and physical properties of anti-HIV antisense oligonucleotides bearing terminal lipophilic groups

C. Mackellar, D. Graham, D.W. Will, S. Burgess, T. Brown

Research output: Contribution to journal › Article › peer-review

137 Citations (Scopus)

Abstract

A number of phosphoramidite monomers have been prepared and used in the synthesis of antisense phosphorothioate oligonucleotides bearing 5'-polyalkyl and cholesterol moieties. Similar groups have also been attached to the 3'-end of oligonucleotides by means of functionalised CPG. Melting temperatures of duplexes formed between phosphorothioate oligonucleotides with lipophilic end-groups and complementary DNA strands were found to be identical to those formed by the equivalent unmodified phosphorothioates.

Original language	English
Pages (from-to)	3411-3417
Number of pages	6
Journal	Nucleic Acids Research
Volume	20
Issue number	13
DOIs	https://doi.org/10.1093/nar/20.13.3411
Publication status	Published - 11 Jul 1992

Keywords

human immunodeficiency virus
phosphorothioate oligodeoxynucleotide
cells
derivatives
cholesterol
inhibitors
infection
invitro

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1093/nar/20.13.3411

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abstract = "A number of phosphoramidite monomers have been prepared and used in the synthesis of antisense phosphorothioate oligonucleotides bearing 5'-polyalkyl and cholesterol moieties. Similar groups have also been attached to the 3'-end of oligonucleotides by means of functionalised CPG. Melting temperatures of duplexes formed between phosphorothioate oligonucleotides with lipophilic end-groups and complementary DNA strands were found to be identical to those formed by the equivalent unmodified phosphorothioates.",

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T1 - Synthesis and physical properties of anti-HIV antisense oligonucleotides bearing terminal lipophilic groups

AU - Mackellar, C.

AU - Graham, D.

AU - Will, D.W.

AU - Burgess, S.

AU - Brown, T.

PY - 1992/7/11

Y1 - 1992/7/11

N2 - A number of phosphoramidite monomers have been prepared and used in the synthesis of antisense phosphorothioate oligonucleotides bearing 5'-polyalkyl and cholesterol moieties. Similar groups have also been attached to the 3'-end of oligonucleotides by means of functionalised CPG. Melting temperatures of duplexes formed between phosphorothioate oligonucleotides with lipophilic end-groups and complementary DNA strands were found to be identical to those formed by the equivalent unmodified phosphorothioates.

AB - A number of phosphoramidite monomers have been prepared and used in the synthesis of antisense phosphorothioate oligonucleotides bearing 5'-polyalkyl and cholesterol moieties. Similar groups have also been attached to the 3'-end of oligonucleotides by means of functionalised CPG. Melting temperatures of duplexes formed between phosphorothioate oligonucleotides with lipophilic end-groups and complementary DNA strands were found to be identical to those formed by the equivalent unmodified phosphorothioates.

KW - human immunodeficiency virus

KW - phosphorothioate oligodeoxynucleotide

KW - cells

KW - derivatives

KW - cholesterol

KW - inhibitors

KW - infection

KW - invitro

UR - http://dx.doi.org/10.1093/nar/20.13.3411

U2 - 10.1093/nar/20.13.3411

DO - 10.1093/nar/20.13.3411

M3 - Article

SN - 0305-1048

VL - 20

SP - 3411

EP - 3417

JO - Nucleic Acids Research

JF - Nucleic Acids Research

IS - 13

ER -

Synthesis and physical properties of anti-HIV antisense oligonucleotides bearing terminal lipophilic groups

Abstract

Keywords

UN SDGs

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